##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547369_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2782426
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.257598944230683	31.0	31.0	33.0	30.0	34.0
2	31.63671882019504	31.0	31.0	34.0	30.0	34.0
3	31.589526909251134	31.0	31.0	34.0	30.0	34.0
4	35.442729114808444	37.0	35.0	37.0	33.0	37.0
5	35.38221034449793	37.0	35.0	37.0	33.0	37.0
6	35.429608550236374	37.0	35.0	37.0	33.0	37.0
7	35.88953380970419	37.0	35.0	37.0	35.0	37.0
8	35.898533509965766	37.0	35.0	37.0	35.0	37.0
9	37.65705143640837	39.0	37.0	39.0	35.0	39.0
10	36.86453763729925	39.0	37.0	39.0	32.0	39.0
11	36.6035032018821	39.0	35.0	39.0	32.0	39.0
12	36.04861728577867	38.0	35.0	39.0	31.0	39.0
13	35.744006489301064	38.0	35.0	39.0	30.0	39.0
14	36.83230173956109	39.0	35.0	41.0	31.0	41.0
15	37.08245035088085	39.0	35.0	41.0	32.0	41.0
16	37.216858238098695	39.0	35.0	41.0	32.0	41.0
17	37.13720939927962	39.0	35.0	41.0	32.0	41.0
18	36.91757876040549	38.0	35.0	40.0	32.0	41.0
19	36.71978230508197	38.0	35.0	40.0	31.0	41.0
20	36.446506394060435	38.0	35.0	40.0	31.0	41.0
21	36.30217083940418	37.0	35.0	40.0	31.0	41.0
22	36.18768297881058	37.0	35.0	40.0	31.0	41.0
23	36.1099781270014	37.0	35.0	40.0	31.0	41.0
24	35.95391719312571	37.0	35.0	40.0	30.0	41.0
25	35.85515841211949	37.0	35.0	40.0	30.0	41.0
26	35.617082718462235	36.0	34.0	40.0	30.0	41.0
27	35.559395290297026	36.0	34.0	40.0	30.0	41.0
28	35.54854468726212	36.0	34.0	40.0	30.0	41.0
29	35.537678630087555	36.0	34.0	40.0	29.0	41.0
30	35.458644003470354	36.0	34.0	40.0	29.0	41.0
31	35.28333943112953	36.0	34.0	40.0	29.0	41.0
32	34.91629894200241	36.0	34.0	40.0	26.0	41.0
33	34.51439642959058	36.0	33.0	40.0	24.0	41.0
34	34.0894755152518	36.0	33.0	40.0	21.0	41.0
35	33.78832680545682	36.0	33.0	40.0	20.0	41.0
36	33.43935508078202	36.0	33.0	40.0	18.0	41.0
37	33.34017220943163	36.0	33.0	40.0	18.0	41.0
38	33.27198135727599	36.0	33.0	40.0	18.0	41.0
39	33.14621053713558	36.0	32.0	40.0	16.0	41.0
40	33.038623848397044	36.0	32.0	40.0	15.0	41.0
41	33.0099179636763	35.0	32.0	40.0	15.0	41.0
42	32.90219075008643	35.0	32.0	40.0	15.0	41.0
43	32.76276781484934	35.0	32.0	40.0	15.0	41.0
44	32.63858769289821	35.0	31.0	40.0	14.0	41.0
45	32.48511838230379	35.0	31.0	40.0	13.0	41.0
46	32.3936431732596	35.0	31.0	40.0	13.0	41.0
47	32.34934082703367	35.0	31.0	40.0	13.0	41.0
48	32.229869545497344	35.0	31.0	39.0	12.0	41.0
49	32.106272368070165	35.0	31.0	39.0	10.0	41.0
50	31.955834584639447	35.0	31.0	39.0	10.0	41.0
51	31.51850830893616	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	11.0
13	14.0
14	45.0
15	145.0
16	334.0
17	988.0
18	2064.0
19	4191.0
20	7414.0
21	11669.0
22	17531.0
23	25755.0
24	38237.0
25	57077.0
26	78116.0
27	84089.0
28	80479.0
29	79415.0
30	85431.0
31	97728.0
32	117288.0
33	146504.0
34	229075.0
35	327846.0
36	216941.0
37	241601.0
38	332380.0
39	499891.0
40	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.383818293819854	23.588983139174232	26.95568543422179	20.071513132784126
2	35.70287224170562	24.307636573263764	27.419956541521678	12.569534643508936
3	25.436651325138566	23.56684418561356	37.910945340505016	13.08555914874286
4	22.468378314463706	26.169860402397045	37.782711921179576	13.579049361959672
5	21.048610097806733	29.814916910638413	36.344614376087634	12.791858615467222
6	18.90544438558294	39.06353664032754	32.98463283479956	9.046386139289957
7	82.35579311004139	3.0116524213042863	13.447940753860122	1.184613714794212
8	82.95893583513092	2.4638570801164166	12.917073086579842	1.6601339981728174
9	78.81945467732116	4.6065915140240925	14.520062707867162	2.053891100787586
10	41.29417997100372	22.880931963689243	22.976747629586555	12.848140435720481
11	34.1254718005079	23.536079665730554	27.969225416956284	14.369223116805262
12	31.656115921860994	21.013137456306115	31.270265588375036	16.06048103345785
13	19.554554191198616	33.26902494441901	32.21796374818234	14.958457116200035
14	16.077300887786414	34.296222073830535	33.530163964827814	16.096313073555237
15	15.828812697983702	25.91080589384947	43.77284427330682	14.487537134860009
16	16.64928375453651	22.18840680758446	44.07287022188551	17.089439215993522
17	16.970837679061365	24.32923642892929	33.4025055832572	25.297420308752145
18	19.615292554051752	25.37803341400634	37.01981651982838	17.98685751211353
19	23.255101842780366	28.5856658901261	30.200515665106636	17.9587166019869
20	25.157147036435113	25.25774270366939	32.373080182545735	17.212030077349766
21	20.3540363697004	29.252853445159012	33.06111285619097	17.331997328949626
22	21.38730733539724	25.85628512672035	30.866553144629904	21.889854393252506
23	18.60416054191558	29.78422427047476	31.198170229864154	20.413444957745508
24	18.65904070764146	25.62519182900102	39.981189077445364	15.734578385912151
25	17.594969282201934	27.673835710275853	36.26137766107706	18.469817346445154
26	17.057884019197637	33.079765643363025	31.83915762719296	18.023192710246384
27	17.050085069647857	32.42709779163938	32.633248826743284	17.889568311969484
28	16.276623349551794	29.030134134744284	38.14218239766305	16.551060118040876
29	16.240431910857648	26.06674894498542	37.80880425930465	19.884014884852284
30	17.22342301286719	31.097538622770198	34.15551752319738	17.523520841165226
31	22.071135045460327	28.55637490448982	30.57881862806055	18.7936714219893
32	23.290251025543895	28.57883731678758	30.713054003951946	17.417857653716577
33	22.237644415341144	29.429749434486308	28.84245618751406	19.490149962658485
34	17.354459741247386	30.465931528816938	30.70130166983776	21.478307060097915
35	18.845856098239448	28.382425983655992	31.68623352426983	21.085484393834733
36	23.05232915448605	30.123424666100735	27.969872334430455	18.85437384498276
37	17.906064707560958	33.34708631963617	30.49637258996286	18.250476382840013
38	18.461012080824432	33.27078599754315	28.381563427023753	19.88663849460866
39	17.86944199055069	33.377814899659505	29.08749415078784	19.665248959001964
40	21.232622179349963	29.92913378469005	27.533849956836224	21.304394079123757
41	16.81396019157383	27.459526327025408	30.965567458038418	24.760946023362347
42	18.979803955253438	29.33062730149876	27.527632361112207	24.16193638213559
43	19.23026883733835	30.232753719236378	28.470406760143845	22.066570683281427
44	18.43768711189444	32.08998190787464	28.33836371569271	21.13396726453821
45	17.096627187928807	35.17042322059958	26.356100755240213	21.376848836231403
46	19.60569661151815	34.04399613862147	26.82547532261415	19.524831927246222
47	19.421756409694275	29.656925287500908	29.181369064262626	21.739949238542195
48	19.673335427429155	28.070611761103443	31.155078338112137	21.100974473355265
49	18.58410610021614	28.490173682965874	31.297436122290407	21.62828409452758
50	17.725251273528926	31.868987710724383	29.6357567101515	20.770004305595187
51	16.61542840672133	33.37537098920151	27.645335401552458	22.363865202524703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2143.0
1	2999.5
2	3856.0
3	36828.5
4	69801.0
5	48901.5
6	28002.0
7	29181.0
8	30360.0
9	33332.0
10	36304.0
11	38172.0
12	40040.0
13	39898.5
14	39757.0
15	38034.0
16	36311.0
17	33784.5
18	31258.0
19	29339.0
20	27420.0
21	26813.5
22	26207.0
23	26152.5
24	26098.0
25	27257.0
26	30446.0
27	32476.0
28	37752.5
29	43029.0
30	48668.5
31	54308.0
32	59892.0
33	65476.0
34	75265.5
35	85055.0
36	89805.0
37	94555.0
38	106106.5
39	117658.0
40	144963.5
41	172269.0
42	194242.0
43	216215.0
44	219568.5
45	222922.0
46	223991.5
47	225061.0
48	220111.0
49	215161.0
50	198180.5
51	181200.0
52	167663.5
53	154127.0
54	135736.5
55	117346.0
56	104369.0
57	91392.0
58	82770.5
59	74149.0
60	65975.5
61	57802.0
62	50987.0
63	44172.0
64	37560.5
65	30949.0
66	25815.0
67	20681.0
68	17065.0
69	13449.0
70	12235.0
71	11021.0
72	8525.5
73	6030.0
74	5172.5
75	3406.5
76	2498.0
77	2057.5
78	1617.0
79	1140.5
80	664.0
81	536.0
82	408.0
83	313.0
84	218.0
85	180.0
86	142.0
87	88.0
88	34.0
89	26.5
90	19.0
91	17.5
92	16.0
93	14.0
94	12.0
95	8.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2782426.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.25907054282009
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.55137440936423	21.957555973459726
2	7.75500034763652	4.227882030716365
3	2.842780344034148	2.3247464980730768
4	1.543644619935483	1.6831327035146417
5	1.0230030410323356	1.3943056030509957
6	0.7284339698552661	1.1913859782042708
7	0.5357638467104373	1.0223097144240723
8	0.39884979785445135	0.8697819820563218
9	0.33191398334771566	0.8142900017601415
>10	2.9500030125143817	17.46742391037102
>50	0.6842112007591801	13.35294479206388
>100	0.6484540220710477	30.480551780394894
>500	0.005093089502637617	0.9192563803904586
>1k	0.0013402867112204254	0.5480610851014376
>5k	0.0	0.0
>10k+	1.3402867112204253E-4	1.74637156641882
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47800	1.7179252925324877	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2791	0.10030814835686555	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050423623126005866	0.0
2	0.0	0.0	0.0	0.14476575477658704	0.0
3	0.0	0.0	0.0	0.21283584900371114	0.0
4	0.0	0.0	0.0	0.39196010963094796	0.0
5	0.0	0.0	0.0	0.6715362780537559	0.0
6	0.0	0.0	0.0	1.293870888210504	0.0
7	0.0	0.0	0.0	1.582072622955651	0.0
8	3.593985967641188E-5	0.0	0.0	2.2071746023074827	0.0
9	3.593985967641188E-5	0.0	0.0	2.445671511120152	0.0
10	3.593985967641188E-5	0.0	0.0	2.8223571803886247	0.0
11	3.593985967641188E-5	0.0	0.0	3.21234059773737	0.0
12	3.593985967641188E-5	0.0	0.0	3.5020518065889266	0.0
13	3.593985967641188E-5	0.0	0.0	3.6733411778067055	0.0
14	3.593985967641188E-5	0.0	0.0	3.748527364249759	0.0
15	3.593985967641188E-5	0.0	0.0	3.8300749058555374	0.0
16	3.593985967641188E-5	0.0	0.0	4.001292397353963	0.0
17	3.593985967641188E-5	0.0	0.0	4.2042447849466615	0.0
18	3.593985967641188E-5	0.0	0.0	4.447737334254352	0.0
19	3.593985967641188E-5	0.0	0.0	4.5987566246146345	0.0
20	3.593985967641188E-5	0.0	0.0	4.750386892589416	0.0
21	3.593985967641188E-5	0.0	0.0	4.975226654725049	0.0
22	3.593985967641188E-5	0.0	0.0	5.2109921342023116	0.0
23	3.593985967641188E-5	0.0	0.0	5.519859288261395	0.0
24	3.593985967641188E-5	0.0	0.0	5.73686416098757	0.0
25	3.593985967641188E-5	0.0	0.0	5.953329935818599	0.0
26	3.593985967641188E-5	0.0	0.0	6.137845175397297	0.0
27	3.593985967641188E-5	0.0	0.0	6.323510490485641	0.0
28	3.593985967641188E-5	0.0	0.0	6.526678517236397	0.0
29	3.593985967641188E-5	0.0	0.0	6.747241436070537	0.0
30	3.593985967641188E-5	0.0	0.0	6.991884060887872	0.0
31	3.593985967641188E-5	0.0	0.0	7.2290871347521914	0.0
32	3.593985967641188E-5	0.0	0.0	7.456658326223231	0.0
33	3.593985967641188E-5	0.0	0.0	7.682648163868509	0.0
34	3.593985967641188E-5	0.0	0.0	7.913166423833014	0.0
35	3.593985967641188E-5	0.0	0.0	8.173910105785383	0.0
36	3.593985967641188E-5	3.593985967641188E-4	0.0	8.417654234110808	0.0
37	3.593985967641188E-5	3.593985967641188E-4	0.0	8.679727690871204	0.0
38	3.593985967641188E-5	3.593985967641188E-4	0.0	8.943741900054125	0.0
39	3.593985967641188E-5	3.593985967641188E-4	0.0	9.20311986733879	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	80	0.0	45.0	1
GTCGACG	20	7.035017E-4	45.0	1
TCCGATT	55	1.8189894E-12	45.0	12
CTACGCT	20	7.035017E-4	45.0	18
CGTCGTC	20	7.035017E-4	45.0	41
TACGTAG	45	3.8562575E-10	45.0	1
GTCGTAA	25	3.892241E-5	44.999996	9
CGTTTTT	29920	0.0	43.766716	1
CGTTGAT	720	0.0	41.25	25
GTTTACG	115	0.0	41.086956	1
TTAATCG	730	0.0	40.993153	20
AGGGCGA	2505	0.0	40.958084	6
TACGGGA	610	0.0	40.573772	4
CCCGTAT	50	1.0822987E-9	40.499996	45
CGTTAGG	395	0.0	40.44304	2
AATCGTT	740	0.0	40.43919	22
TAATCGT	740	0.0	40.43919	21
TCGTTGA	735	0.0	40.408165	24
CGTAAGG	465	0.0	40.161293	2
CTTCGCA	40	3.460591E-7	39.375	24
>>END_MODULE