FastQCFastQC Report
Thu 26 May 2016
SRR1547368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547368_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2308492
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172260.7462014163358591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA207.0347684E-445.038
CGACGGT3100.043.54838628
CGTTTTT89050.042.3469961
TACGGGA10600.039.9056634
GGCGATA3900.039.8076938
CGGTCTA3350.039.62686531
ATAACGC1600.039.37511
ACGGGAT13250.039.0566025
ACGGGAC9150.038.852465
AGGGATC32750.038.748096
TAGGGAC27800.038.687055
CTTACGA356.251026E-638.5714329
CGCATCG1700.038.38235521
TAGGGAT49900.038.3717465
GTAGGGA42900.038.2867134
TCACGAC3650.038.21917725
GGGCGAT23250.037.838717
CGAGGGA21050.037.8384784
AGCGGGA16650.037.7027054
AAGGGAT48100.037.7027055
Produced by FastQC (version 0.11.5)