FastQCFastQC Report
Thu 26 May 2016
SRR1547367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547367_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1066934
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77380.7252557327819715No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT11810.11069100806610344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG207.033059E-445.012
ACCGCGT207.033059E-445.045
TCGATAC253.8906164E-545.024
TCGCGCG253.8906164E-545.01
TAGTACG253.8906164E-545.01
CGACGGT2050.042.8048828
TAACGCC750.042.012
CACGACG2100.041.78571726
CGGTCTA2100.041.78571731
CGTTTTT37900.041.497361
AAACGCG556.184564E-1140.9090921
ACGACGG2100.040.71428727
CTCGTAG501.0822987E-940.51
ATGGGAC9350.040.1871645
ACGTAAG451.9281288E-840.01
TATCCGG451.9281288E-840.02
ATAACGC850.039.70588311
TTAGACG403.457917E-739.3751
GGGCGAT12050.039.0248957
ACGGTCT2250.039.030
Produced by FastQC (version 0.11.5)