##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547367_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1066934
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.406579038628443	31.0	31.0	33.0	30.0	34.0
2	31.828285535937557	31.0	31.0	34.0	30.0	34.0
3	31.950065327377327	31.0	31.0	34.0	30.0	34.0
4	35.7106746996534	37.0	35.0	37.0	33.0	37.0
5	35.592636470484585	37.0	35.0	37.0	33.0	37.0
6	35.632434621073095	37.0	35.0	37.0	33.0	37.0
7	35.98613128834586	37.0	35.0	37.0	35.0	37.0
8	35.9697272746018	37.0	35.0	37.0	35.0	37.0
9	37.68846526589274	39.0	37.0	39.0	35.0	39.0
10	37.07359780455023	39.0	37.0	39.0	33.0	39.0
11	36.81356953663488	39.0	37.0	39.0	32.0	39.0
12	36.49336978669721	39.0	35.0	39.0	32.0	39.0
13	36.36899751999655	39.0	35.0	39.0	32.0	39.0
14	37.47545490161528	39.0	36.0	41.0	32.0	41.0
15	37.60266989335798	40.0	36.0	41.0	32.0	41.0
16	37.66076064686288	40.0	36.0	41.0	32.0	41.0
17	37.54761306697509	39.0	36.0	41.0	32.0	41.0
18	37.43547117253738	39.0	36.0	41.0	32.0	41.0
19	37.40188802681328	39.0	36.0	41.0	32.0	41.0
20	37.2735323834464	39.0	35.0	41.0	32.0	41.0
21	37.172086558306326	39.0	35.0	41.0	32.0	41.0
22	37.08356843066206	39.0	35.0	41.0	31.0	41.0
23	37.0007713691756	39.0	35.0	41.0	31.0	41.0
24	36.884117480556434	39.0	35.0	41.0	31.0	41.0
25	36.789768626737924	38.0	35.0	40.0	31.0	41.0
26	36.69251425111582	38.0	35.0	41.0	31.0	41.0
27	36.624055471097556	38.0	35.0	40.0	31.0	41.0
28	36.70457122933565	39.0	35.0	41.0	31.0	41.0
29	36.71538726856581	39.0	35.0	41.0	31.0	41.0
30	36.67184474391106	39.0	35.0	41.0	31.0	41.0
31	36.596816672821376	39.0	35.0	41.0	30.0	41.0
32	36.49120564158608	39.0	35.0	41.0	30.0	41.0
33	36.329783285564055	39.0	35.0	41.0	30.0	41.0
34	36.156635743166866	38.0	35.0	41.0	30.0	41.0
35	36.01932921811471	38.0	35.0	41.0	29.0	41.0
36	35.90227792909402	38.0	35.0	40.0	29.0	41.0
37	35.82451585571366	38.0	35.0	40.0	28.0	41.0
38	35.73149885559931	38.0	35.0	40.0	28.0	41.0
39	35.694211638208174	38.0	35.0	40.0	28.0	41.0
40	35.554351065764145	38.0	34.0	40.0	27.0	41.0
41	35.47143872067063	38.0	34.0	40.0	27.0	41.0
42	35.402446636811646	38.0	34.0	40.0	27.0	41.0
43	35.36550152118125	38.0	34.0	40.0	27.0	41.0
44	35.23380077867984	38.0	34.0	40.0	26.0	41.0
45	35.139516596153086	38.0	34.0	40.0	26.0	41.0
46	35.08392552866438	38.0	34.0	40.0	26.0	41.0
47	34.94329358704475	38.0	34.0	40.0	26.0	41.0
48	34.88919652012214	37.0	34.0	40.0	25.0	41.0
49	34.78328556405551	37.0	34.0	40.0	25.0	41.0
50	34.718830780535626	37.0	34.0	40.0	25.0	41.0
51	34.2620068345371	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	8.0
13	4.0
14	19.0
15	39.0
16	85.0
17	193.0
18	390.0
19	735.0
20	1347.0
21	2098.0
22	3229.0
23	4797.0
24	6903.0
25	9989.0
26	13772.0
27	16786.0
28	18925.0
29	21435.0
30	25921.0
31	31333.0
32	39141.0
33	49863.0
34	75719.0
35	98596.0
36	88140.0
37	111462.0
38	167196.0
39	278736.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.204085726014917	23.99529867826876	29.627324651759153	15.173290943957172
2	32.012383146473915	24.90735134506914	30.032972986145346	13.047292522311595
3	28.819964496398093	25.657163423417007	32.07517990803555	13.447692172149356
4	26.13460626430501	27.584555370810193	31.91265814005365	14.36818022483115
5	23.235645316392578	32.026629575962524	30.370763327441058	14.366961780203836
6	22.26632575210838	40.7826538473795	26.757512648392495	10.193507752119626
7	89.29296470072188	3.026991360290327	6.28932998667209	1.3907139523157008
8	89.69767577001015	2.7435623946748344	6.1868869114678136	1.3718749238472108
9	84.46858006212193	5.9867808130587274	7.255556576133107	2.2890825486862356
10	35.86379288690772	41.57576757325195	13.102497436579958	9.45794210326037
11	31.221331403816922	23.70793319924194	27.524476678032567	17.54625871890857
12	29.760322569156106	22.393793805427514	29.854142805459382	17.991740819957
13	26.037505600158962	25.62510895706763	29.44783838550463	18.88954705726877
14	20.297694140406062	29.898287991572115	29.746919678255633	20.05709818976619
15	19.324063156671357	28.34402127966678	34.52059827505731	17.811317288604545
16	24.553533770598744	26.62854497091666	31.90272313001554	16.915198128469054
17	23.390481510571412	26.36470484584801	28.70233772660727	21.54247591697331
18	23.666318628893634	27.347146121503297	30.36860761771581	18.61792763188726
19	25.416005113718377	28.20315033544718	27.52785083238513	18.852993718449312
20	26.809062228778913	27.685592548367566	27.84014756301702	17.665197659836505
21	26.287661654797766	26.08989871913352	30.164939911934574	17.457499714134144
22	25.20109022676192	23.230958990902906	30.616889142158747	20.95106164017643
23	22.27926001045988	27.03691137408687	30.40103699010436	20.2827916253489
24	21.954966286574425	26.805125715367584	32.18343402684702	19.056473971210966
25	22.192094356351937	28.177094365724592	29.762478278881353	19.868332999042117
26	21.034009601343666	31.05299859222782	27.268228400257183	20.64476340617133
27	20.19731304841724	29.91600230192308	30.542376566872925	19.34430808278675
28	18.92788119977431	28.82108921451561	32.47061205285425	19.78041753285583
29	20.35224296910587	27.878200525993176	31.710021425880136	20.059535079020822
30	21.25942185739699	27.700213883895348	31.832053341631255	19.208310917076407
31	24.494954701977818	27.046565204595595	28.169971150980285	20.2885089424463
32	23.640731291720012	28.374763574879047	28.74948216103339	19.23502297236755
33	22.83871354741718	27.669471588683088	28.70533697492066	20.786477888979075
34	21.164008270427225	28.370170975899168	29.7978131730735	20.66800758060011
35	21.354554264837375	27.392416025733553	29.680186403282676	21.572843306146396
36	23.913944067768014	28.64638300026056	27.920096275870858	19.519576656100565
37	22.315719622769542	29.80006260930854	28.058061698286867	19.826156069635047
38	21.57827944371442	30.750918051163428	26.662005334912937	21.008797170209217
39	21.55016149077637	28.52772523886201	28.183655221410138	21.738458048951482
40	22.646199296301365	27.6937467547196	28.289753630496357	21.37030031848268
41	20.521981678341866	27.10505054670673	29.316621271793757	23.056346503157645
42	21.185565367679725	28.39772656977845	27.8418346401933	22.574873422348524
43	21.35361699973944	27.68006268428975	28.238391503129527	22.72792881284128
44	21.032041344638	28.23604834038469	29.13713500553924	21.59477530943807
45	21.116301476942343	29.358798201200827	27.593084483201398	21.931815838655435
46	21.890951080385477	29.110797856287267	27.887948083011693	21.11030298031556
47	21.40197987879288	28.053187919777606	29.435091580172717	21.109740621256798
48	21.132891069175788	28.20774293442706	30.01582103485314	20.643544961544013
49	21.699467820877395	27.821214808038736	29.467427226051473	21.011890145032403
50	20.327311717500802	28.727362704722132	29.576712336470674	21.3686132413064
51	19.942658121308344	29.034035844766404	29.24201496999814	21.781291063927103
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1115.0
1	1595.0
2	2075.0
3	7412.5
4	12750.0
5	8728.5
6	4707.0
7	4772.0
8	4837.0
9	5189.5
10	5542.0
11	5758.5
12	5975.0
13	5922.5
14	5870.0
15	5502.0
16	5134.0
17	4917.0
18	4700.0
19	4790.5
20	4881.0
21	5514.0
22	6147.0
23	6371.0
24	6595.0
25	7720.5
26	11373.0
27	13900.0
28	15590.5
29	17281.0
30	19767.0
31	22253.0
32	24355.5
33	26458.0
34	32399.5
35	38341.0
36	40759.0
37	43177.0
38	46492.5
39	49808.0
40	56056.5
41	62305.0
42	70484.5
43	78664.0
44	77490.0
45	76316.0
46	78849.0
47	81382.0
48	80386.5
49	79391.0
50	76730.5
51	74070.0
52	68786.5
53	63503.0
54	59889.5
55	56276.0
56	52569.0
57	48862.0
58	46135.0
59	43408.0
60	39905.5
61	36403.0
62	31159.0
63	25915.0
64	22481.0
65	19047.0
66	15667.0
67	12287.0
68	10018.5
69	7750.0
70	6209.0
71	4668.0
72	3804.0
73	2940.0
74	2348.0
75	1296.5
76	837.0
77	579.5
78	322.0
79	279.5
80	237.0
81	162.0
82	87.0
83	75.5
84	64.0
85	42.5
86	21.0
87	18.5
88	16.0
89	10.0
90	4.0
91	3.5
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1066934.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.03489282845397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.22691352560489	17.237216558374396
2	5.77096940356526	2.543253846476949
3	2.1417638749758607	1.4158061234684214
4	1.2360027572144276	1.089407531635741
5	0.9428369626091676	1.0387655712899033
6	0.6894496962269635	0.9115170100182775
7	0.5740469009847798	0.8854343359194026
8	0.469054619635763	0.8268454619492223
9	0.4644835728443617	0.9211361173382613
>10	7.505301366568531	41.7935786269286
>50	1.875841632351917	27.264800728971316
>100	0.10120065255405625	3.0369599989468123
>500	0.0012810209184057752	0.19608466207532166
>1k	4.27006972801925E-4	0.11112091454460406
>5k	4.27006972801925E-4	0.7280725120627826
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7738	0.7252557327819715	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1181	0.11069100806610344	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01893275497828357	0.0
2	0.0	0.0	0.0	0.07235686556056889	0.0
3	0.0	0.0	0.0	0.11078473457589691	0.0
4	0.0	0.0	0.0	0.1683328115890955	0.0
5	0.0	0.0	0.0	0.2928953431046344	0.0
6	0.0	0.0	0.0	0.45269904230252295	0.0
7	0.0	0.0	0.0	0.5397709699006686	0.0
8	0.0	0.0	0.0	0.7114779358423295	0.0
9	0.0	0.0	0.0	0.7795233819523982	0.0
10	0.0	0.0	0.0	0.8975250577823933	0.0
11	0.0	0.0	0.0	1.07504306733125	0.0
12	0.0	0.0	0.0	1.2376585618229432	0.0
13	0.0	0.0	0.0	1.301861221031479	0.0
14	0.0	0.0	0.0	1.3332596018122957	0.0
15	0.0	0.0	0.0	1.3700941201611345	0.0
16	0.0	0.0	0.0	1.4456376870546819	0.0
17	0.0	0.0	0.0	1.5414261800636215	0.0
18	0.0	0.0	0.0	1.6453688794245942	0.0
19	0.0	0.0	0.0	1.7180069245145435	0.0
20	0.0	0.0	0.0	1.7977681843487976	0.0
21	0.0	0.0	0.0	1.8993677209649331	0.0
22	0.0	0.0	0.0	2.0120269857367	0.0
23	0.0	0.0	0.0	2.1284353109002057	0.0
24	0.0	0.0	0.0	2.222161820693689	0.0
25	0.0	0.0	0.0	2.3097023808408017	0.0
26	0.0	0.0	0.0	2.3887138285967078	0.0
27	0.0	0.0	0.0	2.4712868837247663	0.0
28	0.0	0.0	0.0	2.5621078717146517	0.0
29	0.0	0.0	0.0	2.661926604644711	0.0
30	0.0	0.0	0.0	2.7774913912200754	0.0
31	9.372650979348301E-5	0.0	0.0	2.886026689560929	0.0
32	9.372650979348301E-5	0.0	0.0	2.984908157393053	0.0
33	9.372650979348301E-5	0.0	0.0	3.0892257627932	0.0
34	9.372650979348301E-5	0.0	0.0	3.1931684621541727	0.0
35	9.372650979348301E-5	0.0	0.0	3.3157627369640483	0.0
36	9.372650979348301E-5	0.0	0.0	3.425141573893043	0.0
37	9.372650979348301E-5	0.0	0.0	3.5422997111348966	0.0
38	9.372650979348301E-5	0.0	0.0	3.6810149456292516	0.0
39	9.372650979348301E-5	0.0	0.0	3.856283518943065	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	7.033059E-4	45.0	12
ACCGCGT	20	7.033059E-4	45.0	45
TCGATAC	25	3.8906164E-5	45.0	24
TCGCGCG	25	3.8906164E-5	45.0	1
TAGTACG	25	3.8906164E-5	45.0	1
CGACGGT	205	0.0	42.80488	28
TAACGCC	75	0.0	42.0	12
CACGACG	210	0.0	41.785717	26
CGGTCTA	210	0.0	41.785717	31
CGTTTTT	3790	0.0	41.49736	1
AAACGCG	55	6.184564E-11	40.909092	1
ACGACGG	210	0.0	40.714287	27
CTCGTAG	50	1.0822987E-9	40.5	1
ATGGGAC	935	0.0	40.187164	5
ACGTAAG	45	1.9281288E-8	40.0	1
TATCCGG	45	1.9281288E-8	40.0	2
ATAACGC	85	0.0	39.705883	11
TTAGACG	40	3.457917E-7	39.375	1
GGGCGAT	1205	0.0	39.024895	7
ACGGTCT	225	0.0	39.0	30
>>END_MODULE