FastQCFastQC Report
Thu 26 May 2016
SRR1547365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547365_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3038290
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT818952.695430653426763No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43450.1430080736203588No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT253.892325E-545.042
TCGCGAT207.035119E-445.032
CCCGCGA253.892325E-545.022
CGTTTTT325500.044.122121
TCGTAAG1200.043.1250041
CGGTCTA3400.043.01470631
ACGGGTC1800.042.55
TATCGCG900.042.51
CGACGGT3450.042.391328
CCAATCG2450.042.244924
ACGGGTA1150.041.086965
TAGTACG1050.040.7142871
TACGGGA16050.040.6542054
CTTGACG1000.040.51
ATAACGC2500.040.49999611
CTACGCG451.9301297E-840.01
TACGAAG1800.040.01
TAACGCC2600.039.8076912
GGACCGT2950.039.661028
CGGACCG403.4607183E-739.37544
Produced by FastQC (version 0.11.5)