##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547365_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3038290
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.360542936981	31.0	31.0	33.0	30.0	34.0
2	31.75894104907695	31.0	31.0	34.0	30.0	34.0
3	31.704691783865265	31.0	31.0	34.0	30.0	34.0
4	35.551918348808044	37.0	35.0	37.0	33.0	37.0
5	35.4799637295979	37.0	35.0	37.0	33.0	37.0
6	35.53276876137564	37.0	35.0	37.0	33.0	37.0
7	35.97259050321069	37.0	35.0	37.0	35.0	37.0
8	35.976380134878504	37.0	35.0	37.0	35.0	37.0
9	37.70781228914949	39.0	38.0	39.0	35.0	39.0
10	37.0671917427237	39.0	37.0	39.0	33.0	39.0
11	36.710625713806124	39.0	37.0	39.0	32.0	39.0
12	36.51818654572144	39.0	35.0	39.0	32.0	39.0
13	36.442731602315774	39.0	35.0	39.0	32.0	39.0
14	37.60403220232433	40.0	36.0	41.0	32.0	41.0
15	37.716647851258436	40.0	36.0	41.0	32.0	41.0
16	37.74807671420437	40.0	36.0	41.0	32.0	41.0
17	37.66684220400291	40.0	36.0	41.0	32.0	41.0
18	37.45028519331598	39.0	36.0	41.0	32.0	41.0
19	37.28824239950762	39.0	36.0	41.0	32.0	41.0
20	37.03009126844376	38.0	35.0	41.0	32.0	41.0
21	36.91208640386533	38.0	35.0	41.0	32.0	41.0
22	36.848741232732884	38.0	35.0	41.0	31.0	41.0
23	36.77580514039147	38.0	35.0	40.0	31.0	41.0
24	36.621506176171465	38.0	35.0	40.0	31.0	41.0
25	36.550976371577434	38.0	35.0	40.0	31.0	41.0
26	36.39124968320996	38.0	35.0	40.0	31.0	41.0
27	36.31126159780666	38.0	35.0	40.0	30.0	41.0
28	36.298118349466314	38.0	35.0	40.0	30.0	41.0
29	36.26506620500347	38.0	35.0	40.0	30.0	41.0
30	36.15385463533764	38.0	35.0	40.0	30.0	41.0
31	35.9771282530634	38.0	35.0	40.0	30.0	41.0
32	35.733013965092205	38.0	35.0	40.0	29.0	41.0
33	35.38004337966422	38.0	34.0	40.0	26.0	41.0
34	35.01094563060143	38.0	34.0	40.0	24.0	41.0
35	34.742974172972296	38.0	34.0	40.0	23.0	41.0
36	34.554260455716864	38.0	34.0	40.0	22.0	41.0
37	34.43040921044403	38.0	33.0	40.0	21.0	41.0
38	34.32895115344486	38.0	33.0	40.0	21.0	41.0
39	34.28687386654993	38.0	33.0	40.0	21.0	41.0
40	34.140291084787826	38.0	33.0	40.0	20.0	41.0
41	34.0608747025465	38.0	33.0	40.0	18.0	41.0
42	33.97435465343993	37.0	33.0	40.0	18.0	41.0
43	33.92550381958272	37.0	33.0	40.0	18.0	41.0
44	33.79734390068098	37.0	33.0	40.0	18.0	41.0
45	33.706291038709274	37.0	33.0	40.0	18.0	41.0
46	33.631813289712305	37.0	33.0	40.0	18.0	41.0
47	33.50549684197361	37.0	33.0	40.0	18.0	41.0
48	33.46034183702017	37.0	33.0	40.0	18.0	41.0
49	33.35167149942895	36.0	33.0	40.0	18.0	41.0
50	33.26439872428241	36.0	32.0	40.0	18.0	41.0
51	32.85862508187171	36.0	31.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	4.0
13	9.0
14	32.0
15	95.0
16	279.0
17	730.0
18	1684.0
19	3272.0
20	5654.0
21	9072.0
22	14056.0
23	21325.0
24	32443.0
25	49286.0
26	68723.0
27	76393.0
28	75271.0
29	74606.0
30	81001.0
31	93617.0
32	111774.0
33	139740.0
34	202194.0
35	281540.0
36	258075.0
37	295115.0
38	424218.0
39	717849.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.504063140779845	22.68660990228056	27.75307821175727	20.056248745182323
2	36.15566650978017	24.001955047082404	27.872125438980476	11.970253004156943
3	26.233736740074185	24.10141889023102	38.025204967267776	11.639639402427022
4	23.31900509826251	26.3339246747348	37.392348985778185	12.954721241224505
5	21.169045746126933	30.41065862705667	36.11261597806661	12.30767964874979
6	19.328569688871042	38.72178100181352	32.69984102899986	9.249808280315571
7	82.61578058710657	2.9662079656648968	13.211642074982969	1.2063693722455724
8	83.50071915452442	2.3863094043030784	12.855125745073709	1.2578456960987925
9	79.25082859108248	5.344453623584319	13.662520694206282	1.7421970911269167
10	36.26813108689427	36.72838998252306	18.480823094569644	8.522655836013021
11	23.296722827643183	25.854312787785233	35.01008791129221	15.83887647327938
12	24.31677028855047	23.680655895256873	35.469293582903546	16.53328023328912
13	21.173423208449492	26.337578045545357	35.23060668994731	17.258392056057847
14	16.16718614747111	30.81611696052714	35.63056851057667	17.386128381425078
15	15.862804406425985	29.13586260692692	39.747851587570636	15.253481399076454
16	19.588452715178605	27.736753239486685	38.07269878780498	14.60209525752973
17	19.35292549427474	27.70170062765569	33.68147872652051	19.26389515154906
18	19.474441215288863	29.11891228289597	35.610524340994445	15.796122160820724
19	21.67660098278966	29.480233947384875	32.76073054250911	16.08243452731635
20	22.757965829463284	29.5099546126275	32.43018276728028	15.301896790628938
21	22.024790260310898	27.9453574214443	34.88541251822571	15.144439800019091
22	20.333904926784474	25.76673062808356	35.12943135777032	18.76993308736164
23	18.444453952716824	28.89171211438013	35.04672035914939	17.617113573753656
24	17.932850386236996	28.572848543094963	37.26151881485967	16.232782255808363
25	18.795144637279524	29.91765104713507	34.68046170707865	16.606742608506757
26	17.509980943227934	33.05662066491349	32.46806591865819	16.965332473200387
27	17.195955619772967	31.99497085531664	34.85921357079146	15.949859954118928
28	16.42361328247139	30.709675508262873	36.818934334773836	16.047776874491902
29	16.978991472176784	29.234141573055894	36.729278640287795	17.057588314479528
30	17.705255258714605	30.162361064941134	35.67190755326187	16.46047612308239
31	20.048283738550303	30.64612002145944	32.953766756958686	16.351829483031572
32	20.072606630703454	30.97890589772537	33.042007181671266	15.90648028989991
33	19.984168726487596	30.94589390742819	32.33641291647604	16.73352444960817
34	17.920606657034057	31.02083737892038	32.76718153961603	18.29137442442953
35	17.596575705413244	31.40658067531407	32.213547752189555	18.78329586708313
36	20.764673549924463	31.49663132880666	30.72524347577091	17.013451645497963
37	18.874630137346994	32.34434500985752	31.35826402351323	17.42276082928226
38	18.643941164273325	32.820204786244894	30.362440715007455	18.173413334474326
39	18.95579421319229	31.715471531683942	31.00872530271962	18.320008952404148
40	19.615079534869942	30.970513018836254	31.087684190778365	18.326723255515436
41	17.942000269888656	31.049471906895	31.336837497408084	19.671690325808267
42	19.009278245328787	31.921343913846272	30.062206043531063	19.00717179729387
43	19.148270902382592	31.41872566476538	30.262022387593017	19.170981045259012
44	19.135105602164376	32.46533411886291	30.335616415812844	18.06394386315987
45	18.947170941549356	32.98648252800095	29.052954128802718	19.01339240164698
46	19.749530163348464	32.745458794255974	29.070398151591846	18.43461289080371
47	19.089816969413718	31.91795384904009	30.02998397124698	18.962245210299216
48	19.12223652120107	31.51246260231907	30.67613690595697	18.68916397052289
49	19.23381244055044	30.93118168443434	30.700130665604664	19.134875209410556
50	17.917414071731137	31.956231959424546	31.310210677716743	18.816143291127574
51	17.7860572888039	32.43140055755047	30.401212524150097	19.38132962949554
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5313.0
1	5187.0
2	5061.0
3	57047.0
4	109033.0
5	70784.0
6	32535.0
7	32706.5
8	32878.0
9	36144.5
10	39411.0
11	42334.5
12	45258.0
13	44879.0
14	44500.0
15	43071.5
16	41643.0
17	38864.0
18	36085.0
19	34388.5
20	32692.0
21	31526.0
22	30360.0
23	31771.0
24	33182.0
25	34449.0
26	41622.5
27	47529.0
28	54286.5
29	61044.0
30	68984.5
31	76925.0
32	89763.5
33	102602.0
34	113888.0
35	125174.0
36	128784.0
37	132394.0
38	146401.5
39	160409.0
40	175647.0
41	190885.0
42	199188.5
43	207492.0
44	224208.5
45	240925.0
46	235106.0
47	229287.0
48	219114.5
49	208942.0
50	190535.0
51	172128.0
52	156679.5
53	141231.0
54	125495.5
55	109760.0
56	100282.5
57	90805.0
58	82793.0
59	74781.0
60	65331.5
61	55882.0
62	46642.5
63	37403.0
64	29445.0
65	21487.0
66	16516.5
67	11546.0
68	9086.5
69	6627.0
70	5452.0
71	4277.0
72	3411.5
73	2546.0
74	1920.0
75	917.5
76	541.0
77	427.0
78	313.0
79	259.5
80	206.0
81	160.0
82	114.0
83	75.0
84	36.0
85	26.5
86	17.0
87	13.0
88	9.0
89	6.5
90	4.0
91	3.0
92	2.0
93	3.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3038290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.534772631575596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.06869666358327	18.043298742204442
2	7.130099104622673	3.2135032432654533
3	2.71849339307191	1.8378189153994786
4	1.546508537990283	1.3940087310560567
5	1.0491028366726638	1.1820646945779734
6	0.7715045537859085	1.0431407822274381
7	0.6053583850206999	0.9549129496941487
8	0.5160736385247339	0.9303681684243849
9	0.41081227290743144	0.8331805047806775
>10	3.2625000111998483	16.06163951351739
>50	0.9336931259275826	15.259382465453278
>100	0.9794477966283077	34.86335826296545
>500	0.0056339969704767585	0.8495684890137594
>1k	0.0019274200162157332	0.7975813658068454
>5k	0.0	0.0
>10k+	1.4826307817044103E-4	2.736173171613254
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81895	2.695430653426763	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4345	0.1430080736203588	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.037619845373548934	0.0
2	0.0	0.0	0.0	0.11641416717956482	0.0
3	0.0	0.0	0.0	0.18204318876736586	0.0
4	0.0	0.0	0.0	0.2981282234414753	0.0
5	0.0	0.0	0.0	0.5676877454094243	0.0
6	0.0	0.0	0.0	0.9911167136777596	0.0
7	0.0	0.0	0.0	1.2875663613414123	0.0
8	0.0	0.0	0.0	1.6857837796918662	0.0
9	0.0	0.0	0.0	1.876713546106527	0.0
10	0.0	0.0	0.0	2.1135572970322123	0.0
11	0.0	0.0	0.0	2.3928920544121857	0.0
12	0.0	0.0	0.0	2.6158464136076542	0.0
13	0.0	0.0	0.0	2.726007063183567	0.0
14	0.0	0.0	0.0	2.7855142201698984	0.0
15	0.0	0.0	0.0	2.844988463905684	0.0
16	0.0	0.0	0.0	2.951495742671042	0.0
17	0.0	0.0	0.0	3.089994700966662	0.0
18	0.0	0.0	0.0	3.248669481846697	0.0
19	0.0	0.0	0.0	3.355670459370238	0.0
20	0.0	0.0	0.0	3.469221173752341	0.0
21	0.0	0.0	0.0	3.6116038956123346	0.0
22	0.0	0.0	0.0	3.773273782292013	0.0
23	0.0	0.0	0.0	3.942974502104802	0.0
24	0.0	0.0	0.0	4.0754832488011346	0.0
25	0.0	0.0	0.0	4.193806384512341	0.0
26	0.0	0.0	0.0	4.303045463072978	0.0
27	0.0	0.0	0.0	4.4190975844965426	0.0
28	0.0	0.0	0.0	4.54551737984195	0.0
29	3.2913250545537125E-5	0.0	0.0	4.690993947253225	0.0
30	3.2913250545537125E-5	0.0	0.0	4.864150558373296	0.0
31	3.2913250545537125E-5	0.0	0.0	5.015354031379493	0.0
32	3.2913250545537125E-5	0.0	0.0	5.156617702720938	0.0
33	3.2913250545537125E-5	0.0	0.0	5.306109686698768	0.0
34	3.2913250545537125E-5	0.0	0.0	5.464126202567892	0.0
35	3.2913250545537125E-5	0.0	0.0	5.634781406646502	0.0
36	3.2913250545537125E-5	0.0	0.0	5.802902290433106	0.0
37	6.582650109107425E-5	0.0	0.0	5.968620506929885	0.0
38	6.582650109107425E-5	0.0	0.0	6.150367476442341	0.0
39	6.582650109107425E-5	0.0	0.0	6.364237778487241	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGAT	25	3.892325E-5	45.0	42
TCGCGAT	20	7.035119E-4	45.0	32
CCCGCGA	25	3.892325E-5	45.0	22
CGTTTTT	32550	0.0	44.12212	1
TCGTAAG	120	0.0	43.125004	1
CGGTCTA	340	0.0	43.014706	31
ACGGGTC	180	0.0	42.5	5
TATCGCG	90	0.0	42.5	1
CGACGGT	345	0.0	42.3913	28
CCAATCG	245	0.0	42.2449	24
ACGGGTA	115	0.0	41.08696	5
TAGTACG	105	0.0	40.714287	1
TACGGGA	1605	0.0	40.654205	4
CTTGACG	100	0.0	40.5	1
ATAACGC	250	0.0	40.499996	11
CTACGCG	45	1.9301297E-8	40.0	1
TACGAAG	180	0.0	40.0	1
TAACGCC	260	0.0	39.80769	12
GGACCGT	295	0.0	39.66102	8
CGGACCG	40	3.4607183E-7	39.375	44
>>END_MODULE