FastQCFastQC Report
Thu 26 May 2016
SRR1547364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547364_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2263101
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT428111.891696393576778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA253.8920076E-545.00000420
CGTTTTT207650.043.6563951
TAATGCG1100.038.8636361
CGGTCTA2750.038.45454431
TAGGGAT34000.038.1838235
GTTTTTT245250.038.0733952
TAGGGAC26150.037.8585055
GGGCGAT22350.037.6510057
AGGGCGA11600.037.629316
TTAGGGA30750.037.6097564
GTAGGGA31150.037.5601924
TACGGGA9350.037.5401044
TAAGGGA31450.036.7726554
AGGGATT36400.036.3461536
AAGGGAT34250.036.328475
GACCGAT8200.036.2195139
ACGGGAT10700.035.9579435
ATAGGGC9800.035.8163264
TATGGGA27400.035.802924
TAACGCC1200.035.62500412
Produced by FastQC (version 0.11.5)