##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547364_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2263101
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.323903352081945	31.0	31.0	33.0	30.0	34.0
2	31.732722931941616	31.0	31.0	34.0	30.0	34.0
3	31.737654218702566	31.0	31.0	34.0	30.0	34.0
4	35.574567816460686	37.0	35.0	37.0	33.0	37.0
5	35.4938126932912	37.0	35.0	37.0	33.0	37.0
6	35.53539192462024	37.0	35.0	37.0	33.0	37.0
7	35.91814329099762	37.0	35.0	37.0	35.0	37.0
8	35.900179444046024	37.0	35.0	37.0	35.0	37.0
9	37.60530086814508	39.0	37.0	39.0	35.0	39.0
10	36.98430383796393	39.0	37.0	39.0	32.0	39.0
11	36.741364614305766	39.0	37.0	39.0	32.0	39.0
12	36.5208251863262	39.0	35.0	39.0	32.0	39.0
13	36.431482730996095	39.0	35.0	39.0	32.0	39.0
14	37.570461062056005	40.0	36.0	41.0	32.0	41.0
15	37.64625573494069	40.0	36.0	41.0	32.0	41.0
16	37.67823132948993	40.0	36.0	41.0	32.0	41.0
17	37.58973770945265	40.0	36.0	41.0	32.0	41.0
18	37.383540107136184	39.0	36.0	41.0	32.0	41.0
19	37.243730173774836	39.0	36.0	41.0	32.0	41.0
20	37.00985992229246	39.0	35.0	41.0	32.0	41.0
21	36.88410459807141	39.0	35.0	41.0	31.0	41.0
22	36.81008094645356	38.0	35.0	40.0	31.0	41.0
23	36.71276315109224	38.0	35.0	40.0	31.0	41.0
24	36.57210791741067	38.0	35.0	40.0	31.0	41.0
25	36.48743383525525	38.0	35.0	40.0	31.0	41.0
26	36.37200195660733	38.0	35.0	40.0	30.0	41.0
27	36.281028995170786	38.0	35.0	40.0	30.0	41.0
28	36.31139706093541	38.0	35.0	40.0	30.0	41.0
29	36.28280841199752	38.0	35.0	40.0	30.0	41.0
30	36.19395952721509	38.0	35.0	40.0	30.0	41.0
31	36.046996576820916	38.0	35.0	40.0	30.0	41.0
32	35.8466997274978	38.0	35.0	40.0	29.0	41.0
33	35.50220471821629	38.0	34.0	40.0	26.0	41.0
34	35.133564520540624	38.0	34.0	40.0	24.0	41.0
35	34.876067396019884	38.0	34.0	40.0	23.0	41.0
36	34.7082662240881	38.0	34.0	40.0	23.0	41.0
37	34.60085608198662	38.0	34.0	40.0	22.0	41.0
38	34.500506163887515	38.0	33.0	40.0	22.0	41.0
39	34.435230243811475	38.0	33.0	40.0	22.0	41.0
40	34.28834064409852	38.0	33.0	40.0	21.0	41.0
41	34.178004870308484	38.0	33.0	40.0	20.0	41.0
42	34.09661212645834	38.0	33.0	40.0	20.0	41.0
43	34.043976384615625	38.0	33.0	40.0	20.0	41.0
44	33.89833330461168	37.0	33.0	40.0	19.0	41.0
45	33.81579567151444	37.0	33.0	40.0	20.0	41.0
46	33.72667680320056	37.0	33.0	40.0	20.0	41.0
47	33.60475780798117	37.0	33.0	40.0	19.0	41.0
48	33.52591024439475	37.0	32.0	40.0	19.0	41.0
49	33.40455154233064	36.0	32.0	40.0	18.0	41.0
50	33.34655501455746	36.0	32.0	40.0	18.0	41.0
51	32.926958187018606	36.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	2.0
11	6.0
12	9.0
13	18.0
14	47.0
15	92.0
16	224.0
17	598.0
18	1297.0
19	2487.0
20	4328.0
21	6675.0
22	10332.0
23	14997.0
24	22079.0
25	33542.0
26	47195.0
27	56482.0
28	57738.0
29	58448.0
30	62779.0
31	72089.0
32	85773.0
33	106568.0
34	151882.0
35	188053.0
36	188967.0
37	232528.0
38	335369.0
39	522368.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.25428339256622	21.443806529182748	27.101662718544155	20.20024735970688
2	36.269702501125664	23.30638358606178	27.74745802330519	12.676455889507363
3	27.72757380249489	24.306869202921124	35.746526558028116	12.219030436555858
4	24.5812714501032	26.612466699453535	34.70658180964968	14.099680040793583
5	22.058715010951786	30.081512049175004	34.145625847012575	13.714147092860637
6	20.495682693790513	38.46279949502917	31.171388285365964	9.870129525814358
7	83.02727098790554	3.185584735281368	12.143249461689956	1.643894815123143
8	83.42645776746154	2.8656697160223956	11.900661967804353	1.8072105487117014
9	78.72688845968429	5.428171345423824	13.198659715143071	2.6462804797488046
10	34.05614685336624	35.36762168369861	19.615209396310636	10.961022066624512
11	26.548925567175306	23.567485498879634	32.10563735334835	17.777951580596714
12	26.382781855515947	21.018726075415987	34.367489564098115	18.23100250496995
13	23.409295475544397	22.205239624744983	34.47345036743831	19.912014532272313
14	19.338421042631325	25.62479535822749	35.21826909183461	19.818514507306567
15	18.79703999070302	26.714539032946387	36.968743330500935	17.519677645849654
16	22.616268562472467	26.05924348935377	34.55497567276052	16.76951227541325
17	22.860844478439095	25.350879169776334	32.307042416577964	19.481233935206603
18	22.383313868890518	26.522148149817443	33.328870430440354	17.765667550851685
19	23.47469246843159	27.099188237732207	31.587542933346768	17.838576360489437
20	24.843566416169672	26.735925617106794	31.829909491445584	16.590598475277947
21	25.216550211413452	24.526744497925634	34.06025625900037	16.19644903166054
22	23.90136365986317	22.135379728964814	34.571810979713234	19.39144563145878
23	21.833625631379245	24.46731277128153	34.65603170163417	19.04302989570505
24	20.906313947101786	25.46351223387732	34.88218157298327	18.747992246037626
25	21.944579583500694	26.809143736846035	32.52877357219143	18.71750310746184
26	21.291184087674388	28.312081520002863	31.091983963596853	19.304750428725896
27	19.489717869418996	27.838041695885423	33.77728170329119	18.894958731404387
28	19.08098666387404	27.276555487360042	34.743345524570046	18.899112324195873
29	20.307754713554544	25.939275357131653	34.062200493924045	19.69076943538976
30	20.91024660410649	25.587854894677704	33.82027580739879	19.681622693817026
31	22.300507136004978	25.930040241244207	32.074352845940155	19.695099776810668
32	23.132772244809225	26.151329525284112	31.704859836127508	19.011038393779156
33	22.47504640756201	26.65466543472872	31.37314684585443	19.49714131185484
34	21.265202039148935	27.24814314518	32.06909457421476	19.417560241456304
35	21.430373633346456	27.25141299482436	31.176646557091352	20.14156681473783
36	22.524977895374533	28.123004673675634	30.176558624648216	19.175458806301616
37	21.7273113307802	28.481450894149223	30.44070945132365	19.35052832374693
38	21.670088962003906	28.4371753624783	29.63234959464911	20.260386080868685
39	21.441641358472292	28.27929464924455	29.505576640194143	20.773487352089013
40	22.088232032065736	27.25269442238769	30.825888901997743	19.83318464354883
41	20.542211770486603	27.15764784691448	30.973120510308643	21.32701987229028
42	20.864910580659014	28.206032342347953	29.985095671823746	20.94396140516928
43	20.985453145926762	27.924825272933024	30.10342887922369	20.98629270191653
44	20.97661571445552	27.814092256598354	30.588338744050752	20.62095328489537
45	21.077008935968834	28.0200044098783	29.751831668140312	21.151154986012553
46	21.41702911182488	28.24367980041545	29.603716316682288	20.735574771077385
47	21.233122162908327	27.84749774755965	30.50752043324624	20.411859656285777
48	20.977057586029083	27.706805838537473	30.986509218987575	20.32962735644587
49	21.34659478299908	27.299842119286765	30.783645979565204	20.569917118148947
50	20.410578228722446	27.37557890699531	31.29347740114118	20.92036546314106
51	20.010772828963443	27.802691970000453	31.33766455849739	20.84887064253871
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2698.0
1	3402.0
2	4106.0
3	35457.0
4	66808.0
5	45167.5
6	23527.0
7	23220.0
8	22913.0
9	23327.0
10	23741.0
11	23439.5
12	23138.0
13	22366.5
14	21595.0
15	20225.0
16	18855.0
17	17598.5
18	16342.0
19	15441.0
20	14540.0
21	14646.0
22	14752.0
23	15697.5
24	16643.0
25	18230.5
26	23080.5
27	26343.0
28	31055.5
29	35768.0
30	43240.0
31	50712.0
32	56332.5
33	61953.0
34	67909.5
35	73866.0
36	80907.5
37	87949.0
38	93252.5
39	98556.0
40	107078.5
41	115601.0
42	120066.5
43	124532.0
44	131009.0
45	137486.0
46	142102.0
47	146718.0
48	151214.0
49	155710.0
50	150060.0
51	144410.0
52	135762.5
53	127115.0
54	119723.5
55	112332.0
56	108771.0
57	105210.0
58	99470.0
59	93730.0
60	87321.5
61	80913.0
62	72259.0
63	63605.0
64	54929.0
65	46253.0
66	38219.5
67	30186.0
68	25222.5
69	20259.0
70	17251.5
71	14244.0
72	11556.5
73	8869.0
74	7129.0
75	4204.0
76	3019.0
77	2366.5
78	1714.0
79	1147.0
80	580.0
81	439.0
82	298.0
83	227.5
84	157.0
85	118.5
86	80.0
87	55.0
88	30.0
89	24.0
90	18.0
91	13.5
92	9.0
93	8.5
94	8.0
95	5.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2263101.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.53468942268982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.07757494710937	19.40143741626806
2	5.660231936183999	2.7774406522932935
3	2.229571806261016	1.6410555543659877
4	1.3775418377151853	1.351902446204138
5	0.9700468248803736	1.1899898786953171
6	0.7437880564405184	1.0949165376644523
7	0.5912724110732176	1.0154679478920463
8	0.4823390313633318	0.9467230664752311
9	0.42383725188212956	0.9358843806624965
>10	6.996781781237438	42.98040394856865
>50	1.3850966894066743	21.848001876413804
>100	0.05809318145567417	2.1555840518610854
>500	0.0029137104694158017	0.48365410876952414
>1k	7.284276173539504E-4	0.2647699074889793
>5k	0.0	0.0
>10k+	1.821069043384876E-4	1.912768226377099
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42811	1.891696393576778	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.837431471242335E-5	0.0	0.0	0.023551754870860822	0.0
2	8.837431471242335E-5	0.0	0.0	0.0639830038517945	0.0
3	8.837431471242335E-5	0.0	0.0	0.1048561244062903	0.0
4	8.837431471242335E-5	0.0	0.0	0.1646413483092447	0.0
5	8.837431471242335E-5	0.0	0.0	0.3197382706295477	0.0
6	8.837431471242335E-5	0.0	0.0	0.628120441818549	0.0
7	8.837431471242335E-5	0.0	0.0	0.8240462975359916	0.0
8	8.837431471242335E-5	0.0	0.0	1.1100255799453935	0.0
9	8.837431471242335E-5	0.0	0.0	1.2884533213497762	0.0
10	8.837431471242335E-5	0.0	0.0	1.499226061938906	0.0
11	8.837431471242335E-5	0.0	0.0	1.690247143189809	0.0
12	8.837431471242335E-5	0.0	0.0	1.837611312972775	0.0
13	8.837431471242335E-5	0.0	0.0	1.9193575540817667	0.0
14	8.837431471242335E-5	0.0	0.0	1.972072832807727	0.0
15	8.837431471242335E-5	0.0	0.0	2.0164809259507197	0.0
16	8.837431471242335E-5	0.0	0.0	2.0773708287875796	0.0
17	8.837431471242335E-5	0.0	0.0	2.145684174060283	0.0
18	8.837431471242335E-5	0.0	0.0	2.2352515420213237	0.0
19	8.837431471242335E-5	0.0	0.0	2.300825283537942	0.0
20	8.837431471242335E-5	0.0	0.0	2.370906115104894	0.0
21	8.837431471242335E-5	0.0	0.0	2.4522988589550354	0.0
22	8.837431471242335E-5	0.0	0.0	2.544384894885381	0.0
23	8.837431471242335E-5	0.0	0.0	2.6407570850792785	0.0
24	8.837431471242335E-5	0.0	0.0	2.721310272939652	0.0
25	8.837431471242335E-5	0.0	0.0	2.7946609541509635	0.0
26	8.837431471242335E-5	0.0	0.0	2.8691605014535364	0.0
27	8.837431471242335E-5	0.0	0.0	2.946708962613688	0.0
28	8.837431471242335E-5	0.0	0.0	3.0285877651947484	0.0
29	8.837431471242335E-5	0.0	0.0	3.1167411441203905	0.0
30	8.837431471242335E-5	0.0	0.0	3.224955492485753	0.0
31	8.837431471242335E-5	0.0	0.0	3.31911832481184	0.0
32	8.837431471242335E-5	0.0	0.0	3.42304651891365	0.0
33	8.837431471242335E-5	0.0	0.0	3.519286147635479	0.0
34	8.837431471242335E-5	0.0	0.0	3.618795626001668	0.0
35	8.837431471242335E-5	0.0	0.0	3.7269657872096738	0.0
36	8.837431471242335E-5	0.0	0.0	3.8323079703468825	0.0
37	1.3256147206863503E-4	0.0	0.0	3.9461340876964837	0.0
38	1.3256147206863503E-4	0.0	0.0	4.076530389054664	0.0
39	1.3256147206863503E-4	0.0	0.0	4.251467345027907	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCATA	25	3.8920076E-5	45.000004	20
CGTTTTT	20765	0.0	43.656395	1
TAATGCG	110	0.0	38.863636	1
CGGTCTA	275	0.0	38.454544	31
TAGGGAT	3400	0.0	38.183823	5
GTTTTTT	24525	0.0	38.073395	2
TAGGGAC	2615	0.0	37.858505	5
GGGCGAT	2235	0.0	37.651005	7
AGGGCGA	1160	0.0	37.62931	6
TTAGGGA	3075	0.0	37.609756	4
GTAGGGA	3115	0.0	37.560192	4
TACGGGA	935	0.0	37.540104	4
TAAGGGA	3145	0.0	36.772655	4
AGGGATT	3640	0.0	36.346153	6
AAGGGAT	3425	0.0	36.32847	5
GACCGAT	820	0.0	36.219513	9
ACGGGAT	1070	0.0	35.957943	5
ATAGGGC	980	0.0	35.816326	4
TATGGGA	2740	0.0	35.80292	4
TAACGCC	120	0.0	35.625004	12
>>END_MODULE