FastQCFastQC Report
Thu 26 May 2016
SRR1547363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547363_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences740654
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113091.5268937992638938No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8930.1205691186437932No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG406.8102963E-945.01
CGAGTAG207.03166E-445.01
CGACGGT406.8102963E-945.028
CGGTTTA253.8894577E-545.019
CCGGATT207.03166E-445.020
CGACATC207.03166E-445.029
TTCCGCG207.03166E-445.01
TATTGCG502.1827873E-1145.01
CTAGTCG253.8894577E-545.01
GGCGACT253.8894577E-545.039
TATCGCG253.8894577E-545.01
TCGCGAG207.03166E-445.01
TCGCGAA207.03166E-445.035
AATCGGT207.03166E-445.026
TAACGGA302.1644519E-644.99999617
CGTTTTT46700.043.6027831
ACGGGAG4000.041.6255
CGCCGGT603.6379788E-1241.24999628
TAAACGG501.0804797E-940.52
ACTACGG501.0804797E-940.52