##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547363_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 740654 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45425799361105 31.0 31.0 33.0 30.0 34.0 2 31.880374641870564 31.0 31.0 34.0 30.0 34.0 3 31.92391724070889 31.0 31.0 34.0 30.0 34.0 4 35.70199850402482 37.0 35.0 37.0 33.0 37.0 5 35.60956803041636 37.0 35.0 37.0 33.0 37.0 6 35.6614910606032 37.0 35.0 37.0 33.0 37.0 7 36.040915191168885 37.0 35.0 37.0 35.0 37.0 8 36.05006116216209 37.0 35.0 37.0 35.0 37.0 9 37.795987060084734 39.0 38.0 39.0 35.0 39.0 10 37.20291661153521 39.0 37.0 39.0 33.0 39.0 11 36.85033767454169 39.0 37.0 39.0 32.0 39.0 12 36.67403402938484 39.0 35.0 39.0 32.0 39.0 13 36.585000553564825 39.0 35.0 39.0 32.0 39.0 14 37.72336475601293 40.0 36.0 41.0 33.0 41.0 15 37.83368482449295 40.0 36.0 41.0 33.0 41.0 16 37.89233164203528 40.0 36.0 41.0 33.0 41.0 17 37.80845306985448 40.0 36.0 41.0 33.0 41.0 18 37.64895889308638 39.0 36.0 41.0 33.0 41.0 19 37.56297272410599 39.0 36.0 41.0 33.0 41.0 20 37.39070605167865 39.0 35.0 41.0 32.0 41.0 21 37.285320811066974 39.0 35.0 41.0 32.0 41.0 22 37.22175671771164 39.0 35.0 41.0 32.0 41.0 23 37.14230936442657 39.0 35.0 41.0 32.0 41.0 24 37.020558857442204 39.0 35.0 41.0 32.0 41.0 25 36.949517588509615 39.0 35.0 41.0 32.0 41.0 26 36.852026722329185 39.0 35.0 41.0 31.0 41.0 27 36.75288191247195 38.0 35.0 41.0 31.0 41.0 28 36.796654038187874 39.0 35.0 41.0 31.0 41.0 29 36.812446837524675 39.0 35.0 41.0 31.0 41.0 30 36.76257469749708 39.0 35.0 41.0 31.0 41.0 31 36.65880559613531 39.0 35.0 41.0 31.0 41.0 32 36.51161946063884 39.0 35.0 41.0 30.0 41.0 33 36.27932340877117 39.0 35.0 41.0 30.0 41.0 34 36.02414082689083 38.0 35.0 41.0 29.0 41.0 35 35.84893080979783 38.0 35.0 41.0 29.0 41.0 36 35.70834964774375 38.0 35.0 41.0 27.0 41.0 37 35.62485992109676 38.0 35.0 40.0 27.0 41.0 38 35.5452627002622 38.0 35.0 40.0 27.0 41.0 39 35.5011530350204 38.0 35.0 40.0 27.0 41.0 40 35.36744957834562 38.0 35.0 40.0 26.0 41.0 41 35.30324956052354 38.0 34.0 40.0 25.0 41.0 42 35.228784290640434 38.0 34.0 40.0 25.0 41.0 43 35.18874400192262 38.0 34.0 40.0 25.0 41.0 44 35.07951216087404 38.0 34.0 40.0 24.0 41.0 45 34.987122192008684 38.0 34.0 40.0 24.0 41.0 46 34.91596210916298 38.0 34.0 40.0 24.0 41.0 47 34.80408800870582 38.0 34.0 40.0 24.0 41.0 48 34.76484701358529 38.0 34.0 40.0 24.0 41.0 49 34.6428588787747 38.0 34.0 40.0 24.0 41.0 50 34.58189924040105 37.0 34.0 40.0 24.0 41.0 51 34.17533423163852 37.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 5.0 13 3.0 14 6.0 15 10.0 16 42.0 17 90.0 18 259.0 19 519.0 20 818.0 21 1430.0 22 2219.0 23 3360.0 24 5140.0 25 8026.0 26 11187.0 27 13077.0 28 13745.0 29 14729.0 30 16724.0 31 20184.0 32 25220.0 33 32594.0 34 49426.0 35 67908.0 36 61664.0 37 76243.0 38 114604.0 39 201358.0 40 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.610930339942808 24.23722817941981 29.526202518314893 17.625638962322487 2 33.17527482468197 24.858840970277623 29.562521771299423 12.403362433740991 3 28.122848185522525 24.59812544048908 34.733222260326684 12.54580411366171 4 25.120906658169673 27.031380374641873 34.342891552600804 13.504821414587648 5 21.759688059471763 31.626643479951504 33.59476894744375 13.018899513132986 6 20.568173533120728 40.43034939391403 29.209995490471936 9.79148158249331 7 87.13285825770197 2.8743515865707874 8.833139360619128 1.1596507951081072 8 88.4493974244384 2.1196132067065054 8.192624356312123 1.2383650125429686 9 84.07029463150135 4.941713674671304 9.325677036781007 1.66231465704634 10 38.05380110010883 40.10145087989804 13.713420841580549 8.131327178412592 11 24.453253475982038 26.26125019239753 32.06733508493845 17.218161246681987 12 25.80570684827193 24.040915191168885 32.41446073335187 17.738917227207306 13 22.713169712173293 26.47227990397675 32.48777431837268 18.32677606547727 14 16.90154917140797 30.950079254280677 33.00596499850133 19.14240657581003 15 16.642858878774707 29.423050439206435 37.43016307209574 16.50392760992312 16 21.154817229097528 27.73346258846911 35.77932475892927 15.332395423504092 17 20.900312426585153 27.495159683198906 31.202963867068835 20.401564023147113 18 20.454490220804857 29.15504405565892 32.50532637371836 17.885139349817862 19 22.828743245834087 29.80298492953525 30.08246765696263 17.285804167668033 20 24.252484965989517 29.219986660437936 30.564609115727453 15.962919257845092 21 23.197201392283038 27.625854987619054 32.17845309685764 16.99849052324027 22 21.972337960775207 24.555460444418042 33.30137959155017 20.170822003256582 23 19.705692536596036 28.827225668125738 32.27768971746591 19.18939207781231 24 18.691048721805323 28.767008616709017 34.90914786121454 17.632794800271114 25 19.850024437861673 30.26298379540244 31.893434721205853 17.993557045530032 26 18.931511880041153 32.91590945299694 29.919638589678854 18.23294007728305 27 18.984843125129952 31.06781304090709 32.97585647279296 16.97148736116999 28 17.323068531325017 30.476713823188696 34.928995185336206 17.271222460150085 29 18.252922417215057 29.182857312591302 34.65437302708147 17.909847243112168 30 19.062747247702706 28.971692585201726 33.8376623902659 18.127897776829666 31 21.428089229248744 29.276963332406226 31.436676234787093 17.858271203557937 32 21.946819972618794 29.578318621110533 31.171235151636257 17.30362625463442 33 21.528135944719125 29.17934690152217 30.97465213176463 18.317865021994077 34 19.559200382364775 29.24739486993927 31.73033562230137 19.463069125394583 35 19.468874805239693 29.631244818768277 31.498783507548733 19.401096868443297 36 21.027227288315462 30.275135218334064 30.261228589867873 18.436408903482597 37 19.99125097548923 31.368763282180346 30.502501842965813 18.137483899364614 38 19.753083086029374 31.92934892675932 28.98789448244389 19.32967350476741 39 19.658977066214455 30.800346720600984 29.909242372281795 19.63143384090277 40 20.492699695134302 30.12445757398191 30.470773127533235 18.912069603350552 41 18.640687824544255 29.8322833603815 31.034734167371 20.492294647703247 42 20.2016056080167 30.693009151371626 29.245099601163293 19.86028563944838 43 19.935489445814106 30.565689242210265 29.442762747517737 20.0560585644579 44 19.700021872561276 30.777394032841244 30.19979639615799 19.322787698439488 45 19.530171983139226 31.693611321885797 28.838161948764203 19.93805474621078 46 20.411960240544165 31.463679396857376 28.910530423112547 19.213829939485912 47 19.87797271060441 30.885541696932712 29.90910735647144 19.327378235991436 48 20.45584037890837 29.968244281405354 30.052494147064625 19.523421192621658 49 19.568111425847967 29.810410799104574 30.81938394986053 19.80209382518693 50 18.315569753218103 31.1272199974617 31.14828246387652 19.408927785443673 51 18.546581804729335 31.561565859362133 30.10447523404991 19.787377101858628 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1106.0 1 1135.5 2 1165.0 3 8681.0 4 16197.0 5 10595.5 6 4994.0 7 5143.5 8 5293.0 9 5699.0 10 6105.0 11 6467.0 12 6829.0 13 6754.5 14 6680.0 15 6494.5 16 6309.0 17 6048.5 18 5788.0 19 5562.0 20 5336.0 21 5605.0 22 5874.0 23 6262.0 24 6650.0 25 7491.5 26 9599.0 27 10865.0 28 13506.0 29 16147.0 30 18737.5 31 21328.0 32 24280.0 33 27232.0 34 28882.0 35 30532.0 36 33280.0 37 36028.0 38 38197.0 39 40366.0 40 43660.0 41 46954.0 42 51181.5 43 55409.0 44 56358.5 45 57308.0 46 57388.5 47 57469.0 48 55706.5 49 53944.0 50 48495.5 51 43047.0 52 40046.5 53 37046.0 54 33199.5 55 29353.0 56 27300.0 57 25247.0 58 24201.5 59 23156.0 60 19948.0 61 16740.0 62 13826.5 63 10913.0 64 8585.0 65 6257.0 66 4947.0 67 3637.0 68 2897.0 69 2157.0 70 1713.0 71 1269.0 72 1008.0 73 747.0 74 575.5 75 315.0 76 226.0 77 167.5 78 109.0 79 80.5 80 52.0 81 38.5 82 25.0 83 17.5 84 10.0 85 8.5 86 7.0 87 7.0 88 7.0 89 4.5 90 2.0 91 2.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 740654.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.4871818337444 #Duplication Level Percentage of deduplicated Percentage of total 1 75.38429435681438 19.967175120374545 2 6.793722041224927 3.598931020676836 3 3.059514549260749 2.43113754567768 4 1.8004604457881865 1.9075649284822478 5 1.2653495074070942 1.675777124296531 6 0.9805868985880326 1.558379009201322 7 0.8009650378228184 1.4850714619499465 8 0.6886428857734789 1.4592167467197288 9 0.5944050222810088 1.4169702517243177 >10 7.80117841490266 48.13588348991846 >50 0.7975635948505018 13.293541961051 >100 0.03126695326853861 1.2327565848569864 >500 0.001537719013206817 0.3022154358911511 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 5.125730044022724E-4 1.5353793191792577 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11309 1.5268937992638938 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 893 0.1205691186437932 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.027948272742738176 0.0 2 0.0 0.0 0.0 0.08195459688329504 0.0 3 0.0 0.0 0.0 0.1275899407820656 0.0 4 0.0 0.0 0.0 0.20859942699290088 0.0 5 0.0 0.0 0.0 0.37439884210441043 0.0 6 0.0 0.0 0.0 0.6949263758786154 0.0 7 0.0 0.0 0.0 0.8983952020781634 0.0 8 0.0 0.0 0.0 1.2351246330945354 0.0 9 0.0 0.0 0.0 1.4075398229132632 0.0 10 0.0 0.0 0.0 1.618164487061435 0.0 11 0.0 0.0 0.0 1.8404005108998265 0.0 12 0.0 0.0 0.0 2.035093309426534 0.0 13 0.0 0.0 0.0 2.141890815414485 0.0 14 0.0 0.0 0.0 2.1949520288825823 0.0 15 0.0 0.0 0.0 2.2497684478852475 0.0 16 0.0 0.0 0.0 2.3296978076132717 0.0 17 0.0 0.0 0.0 2.4325798551010323 0.0 18 0.0 0.0 0.0 2.5582795745381786 0.0 19 0.0 0.0 0.0 2.647254993559746 0.0 20 0.0 0.0 0.0 2.7412259975643147 0.0 21 0.0 0.0 0.0 2.8472134086901577 0.0 22 0.0 0.0 0.0 2.979798934455225 0.0 23 0.0 0.0 0.0 3.115894871289428 0.0 24 0.0 0.0 0.0 3.2168866974322694 0.0 25 0.0 0.0 0.0 3.313558017643866 0.0 26 0.0 0.0 0.0 3.4017233418033253 0.0 27 0.0 0.0 0.0 3.4940741560836774 0.0 28 0.0 0.0 0.0 3.6004666146405744 0.0 29 0.0 0.0 0.0 3.715365069249609 0.0 30 0.0 0.0 0.0 3.855511480394354 0.0 31 0.0 0.0 0.0 3.9789159310555267 0.0 32 0.0 0.0 0.0 4.092869274992101 0.0 33 0.0 0.0 0.0 4.206687603118326 0.0 34 0.0 0.0 0.0 4.326581642710361 0.0 35 0.0 0.0 0.0 4.455791773216644 0.0 36 0.0 0.0 0.0 4.576765939291491 0.0 37 0.0 0.0 0.0 4.714347050039559 0.0 38 0.0 0.0 0.0 4.850578002684114 0.0 39 0.0 0.0 0.0 5.049726322952417 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAGCG 40 6.8102963E-9 45.0 1 CGAGTAG 20 7.03166E-4 45.0 1 CGACGGT 40 6.8102963E-9 45.0 28 CGGTTTA 25 3.8894577E-5 45.0 19 CCGGATT 20 7.03166E-4 45.0 20 CGACATC 20 7.03166E-4 45.0 29 TTCCGCG 20 7.03166E-4 45.0 1 TATTGCG 50 2.1827873E-11 45.0 1 CTAGTCG 25 3.8894577E-5 45.0 1 GGCGACT 25 3.8894577E-5 45.0 39 TATCGCG 25 3.8894577E-5 45.0 1 TCGCGAG 20 7.03166E-4 45.0 1 TCGCGAA 20 7.03166E-4 45.0 35 AATCGGT 20 7.03166E-4 45.0 26 TAACGGA 30 2.1644519E-6 44.999996 17 CGTTTTT 4670 0.0 43.602783 1 ACGGGAG 400 0.0 41.625 5 CGCCGGT 60 3.6379788E-12 41.249996 28 TAAACGG 50 1.0804797E-9 40.5 2 ACTACGG 50 1.0804797E-9 40.5 2 >>END_MODULE