FastQCFastQC Report
Thu 26 May 2016
SRR1547362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547362_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3713719
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT965232.599092715415464No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT57800.1556391315551877No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC50850.1369247377090189No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT40800.10986291639189717No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37640.10135392580860318No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT464000.043.8556061
GCGTAAG2300.043.043481
GTTTTTT536050.038.473092
GGGCGAT39750.038.377367
TCGACGG1350.038.3333362
ACGGGAC12150.037.5925945
CGTAGTA301.14041235E-437.50000420
CGGTCTA4950.037.27272831
CGACGGT5050.036.98019828
GTAGGGA51900.036.9797674
GCGATCG3000.036.7500049
TAGGGAC39000.036.5769275
AGGGCGA21300.036.5492976
AGGGATT54150.036.440446
CGGGATC9600.036.328136
TAAGGGA49350.036.2917944
TATGGGA40650.036.2546164
TAGGGAG49700.036.2173045
CGAGGGA24100.036.1307074
AATGCGG4800.036.0937542
Produced by FastQC (version 0.11.5)