##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547362_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3713719
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.357477773628	31.0	31.0	33.0	30.0	34.0
2	31.760146096137053	31.0	31.0	34.0	30.0	34.0
3	31.68132645469407	31.0	31.0	34.0	30.0	34.0
4	35.55448621718552	37.0	35.0	37.0	33.0	37.0
5	35.49015474784172	37.0	35.0	37.0	33.0	37.0
6	35.544202186541305	37.0	35.0	37.0	33.0	37.0
7	35.95038127548153	37.0	35.0	37.0	35.0	37.0
8	35.940168332606746	37.0	35.0	37.0	35.0	37.0
9	37.63908066280728	39.0	37.0	39.0	35.0	39.0
10	37.037891127465485	39.0	37.0	39.0	33.0	39.0
11	36.78583759298967	39.0	37.0	39.0	32.0	39.0
12	36.52656918846041	39.0	35.0	39.0	32.0	39.0
13	36.42675872891837	39.0	35.0	39.0	32.0	39.0
14	37.54169041868811	40.0	36.0	41.0	32.0	41.0
15	37.642135821261654	40.0	36.0	41.0	32.0	41.0
16	37.67640308811733	40.0	36.0	41.0	32.0	41.0
17	37.57731912403712	40.0	36.0	41.0	32.0	41.0
18	37.31323856220678	39.0	36.0	41.0	32.0	41.0
19	37.13856514184299	38.0	36.0	41.0	32.0	41.0
20	36.84440152849475	38.0	35.0	40.0	32.0	41.0
21	36.74222147663838	38.0	35.0	40.0	31.0	41.0
22	36.679279450060704	38.0	35.0	40.0	31.0	41.0
23	36.5922284373158	38.0	35.0	40.0	31.0	41.0
24	36.45253262295828	38.0	35.0	40.0	31.0	41.0
25	36.35746754129755	38.0	35.0	40.0	31.0	41.0
26	36.22032900173654	38.0	35.0	40.0	30.0	41.0
27	36.14384475508244	38.0	35.0	40.0	30.0	41.0
28	36.12880134442051	38.0	35.0	40.0	30.0	41.0
29	36.070169283136394	38.0	35.0	40.0	30.0	41.0
30	35.948939324703886	38.0	35.0	40.0	30.0	41.0
31	35.74908844745658	38.0	35.0	40.0	29.0	41.0
32	35.46803029523774	38.0	34.0	40.0	27.0	41.0
33	35.049238781932615	38.0	34.0	40.0	24.0	41.0
34	34.57659235930344	38.0	34.0	40.0	22.0	41.0
35	34.26740149160451	38.0	33.0	40.0	20.0	41.0
36	34.076253749947156	38.0	33.0	40.0	18.0	41.0
37	33.96994737620159	38.0	33.0	40.0	18.0	41.0
38	33.86060442375958	38.0	33.0	40.0	18.0	41.0
39	33.79393944452986	37.0	33.0	40.0	18.0	41.0
40	33.645286032680445	37.0	33.0	40.0	18.0	41.0
41	33.529743095802345	37.0	33.0	40.0	16.0	41.0
42	33.43805468318955	37.0	33.0	40.0	16.0	41.0
43	33.38013269178417	37.0	33.0	40.0	15.0	41.0
44	33.23457348280793	37.0	32.0	40.0	15.0	41.0
45	33.157283036223255	37.0	32.0	40.0	15.0	41.0
46	33.077338107702815	36.0	32.0	40.0	15.0	41.0
47	32.9598752625064	36.0	32.0	40.0	15.0	41.0
48	32.889269220422975	36.0	32.0	40.0	15.0	41.0
49	32.760809312713214	36.0	31.0	40.0	15.0	41.0
50	32.69556474251283	36.0	31.0	40.0	14.0	41.0
51	32.28551083159496	35.0	31.0	39.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	5.0
12	12.0
13	27.0
14	69.0
15	169.0
16	438.0
17	1068.0
18	2317.0
19	4517.0
20	7958.0
21	12670.0
22	19413.0
23	29070.0
24	43356.0
25	67041.0
26	94235.0
27	107112.0
28	104438.0
29	100182.0
30	105445.0
31	119477.0
32	141514.0
33	175376.0
34	254637.0
35	324551.0
36	311893.0
37	360472.0
38	511878.0
39	814110.0
40	264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.274751266856754	21.92853040308112	25.95333680334996	22.84338152671217
2	38.89351886882125	23.030983227325493	26.400570425495307	11.674927478357947
3	26.076959511476232	23.361972190141472	38.358502622303945	12.202565676078345
4	23.6435228405811	25.17102128620932	37.83379948779108	13.351656385418497
5	20.899481086210344	29.079798444631916	36.79195975785998	13.228760711297758
6	19.87899461429365	36.624311101620776	33.87194884696446	9.624745437121119
7	79.37611865625806	3.233766475061791	15.62921158008993	1.7609032885902245
8	79.77795304383557	3.0172719045248173	15.391929222431747	1.8128458292078642
9	74.66720018396653	5.71561822528845	16.870097064425178	2.7470845263198425
10	33.63493576116017	33.71628278822388	22.58959280440981	10.059188646206135
11	26.711310144897876	23.328151645291417	33.93584705789534	16.02469115191537
12	25.44931374721674	21.13172806020057	36.273288312874506	17.145669879708183
13	22.630979888354506	22.561507750047863	37.16113146955922	17.64638089203841
14	18.01005945791806	26.537225891350424	37.714996745849646	17.737717904881872
15	17.77218470218129	26.886067578080088	39.77094120475997	15.57080651497865
16	22.064243417447578	25.698390212075818	37.19352487358359	15.043841496893007
17	21.364190451673913	26.250020531978862	34.72414041019258	17.66164860615464
18	21.31259796446635	26.869830485289814	35.97498895312219	15.842582597121647
19	22.823078428927985	27.980926936044437	33.19618420241273	15.999810432614852
20	23.07457834047218	28.04288638962722	33.653488591894	15.229046678006602
21	23.125336084932652	26.361472152308778	35.5034939369403	15.009697825818268
22	21.84696795853429	23.81873803591494	36.53911348704627	17.7951805185045
23	19.736576730765037	26.014299951073305	36.07669293234087	18.17243038582079
24	19.78065653324875	26.416053557094653	36.84683736168515	16.956452547971455
25	20.39917398166097	27.552892397082278	34.311212022234315	17.736721599022435
26	18.55552345236675	29.458421598403113	33.83861299145143	18.147441957778714
27	18.138152078819104	28.097817847823165	35.70245890978828	18.061571163569457
28	17.72137310335004	27.6856972754266	36.434743716473974	18.15818590474939
29	19.0597349987977	27.06133662778471	36.495545301084974	17.383383072332613
30	19.474359799435554	26.308075543680072	36.385386185653786	17.83217847123059
31	20.835717511206422	26.615449364908866	34.579649133388926	17.96918399049578
32	21.37248402477409	26.621507981621654	34.109042714324914	17.896965279279343
33	21.06195972285464	26.977269954996597	33.57701538538592	18.383754936762852
34	18.989805098339428	27.694421683493015	34.58481915298384	18.730954065183713
35	19.167982284066188	28.457645826192017	33.46987211471843	18.904499775023368
36	20.918383970354245	29.47724370099084	31.304603283124006	18.29976904553091
37	20.083048825180366	30.03854088044895	30.966963305516654	18.91144698885403
38	20.046885615201365	30.357493391395522	30.251427208143646	19.344193785259467
39	19.785611135360536	29.21510755121753	30.689828713481017	20.309452599940922
40	21.16169801753983	28.22157519187639	30.896037099198942	19.720689691384834
41	18.83699332125021	28.486269424261774	31.681179970805545	20.995557283682476
42	19.498648120657485	29.136345533951275	30.413178810782398	20.95182753460884
43	19.770693474654383	28.333619210284894	31.183350167312067	20.71233714774866
44	19.670847471227628	29.6132259872112	30.38638626131918	20.32954028024199
45	19.25323375301147	29.633609866551563	30.015221937901064	21.097934442535905
46	19.622755518120787	30.08445173153919	30.076131231253626	20.216661519086394
47	19.260100185285964	29.25824490221258	31.790100435708784	19.69155447679267
48	19.517470223245216	28.613015685893306	31.890942744994977	19.978571345866502
49	19.878725342439747	28.30494175784436	31.918193056609827	19.898139843106062
50	18.63275062006576	28.841896761709755	32.18991528438204	20.33543733384244
51	18.174719196578955	29.38779159112469	31.903760085240695	20.533729127055654
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4928.0
1	5846.5
2	6765.0
3	75040.0
4	143315.0
5	96238.0
6	49161.0
7	48946.5
8	48732.0
9	51411.5
10	54091.0
11	55076.0
12	56061.0
13	55264.5
14	54468.0
15	50917.5
16	47367.0
17	44146.0
18	40925.0
19	37964.0
20	35003.0
21	34590.5
22	34178.0
23	34902.0
24	35626.0
25	38626.5
26	45335.0
27	49043.0
28	57040.5
29	65038.0
30	72952.0
31	80866.0
32	90120.0
33	99374.0
34	110795.0
35	122216.0
36	130485.5
37	138755.0
38	147346.0
39	155937.0
40	170571.0
41	185205.0
42	200493.5
43	215782.0
44	221808.5
45	227835.0
46	240776.0
47	253717.0
48	254187.0
49	254657.0
50	241709.0
51	228761.0
52	209543.0
53	190325.0
54	177254.5
55	164184.0
56	154679.0
57	145174.0
58	135511.5
59	125849.0
60	117585.0
61	109321.0
62	98027.0
63	86733.0
64	73543.0
65	60353.0
66	50078.0
67	39803.0
68	32454.0
69	25105.0
70	20448.5
71	15792.0
72	12997.5
73	10203.0
74	7696.0
75	4348.5
76	3508.0
77	2604.0
78	1700.0
79	1071.0
80	442.0
81	343.0
82	244.0
83	205.0
84	166.0
85	108.0
86	50.0
87	62.0
88	74.0
89	52.5
90	31.0
91	30.5
92	30.0
93	19.0
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3713719.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.45777376658244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.10440555356104	19.591754287355663
2	6.644867073943028	3.250373112070225
3	2.4947198829191404	1.8304588352229416
4	1.4938595185377317	1.4614591257380645
5	1.0583748477740564	1.29427462935495
6	0.7952363793959156	1.1669826874932854
7	0.6222385932953023	1.0652999520557105
8	0.5112057597783508	1.0002363856666237
9	0.4131411271582949	0.90940609995376
>10	3.9465066218704594	22.164026886439643
>50	1.341196594659346	23.589623631111152
>100	0.5686471910484411	18.348490979090716
>500	0.0032484965141299435	0.5190615551881997
>1k	0.002016308181183884	0.8664541720880704
>5k	2.2403424235376484E-4	0.2976672541496517
>10k+	1.1201712117688242E-4	2.6444304070213374
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96523	2.599092715415464	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT	5780	0.1556391315551877	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC	5085	0.1369247377090189	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT	4080	0.10986291639189717	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3764	0.10135392580860318	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6927185390170878E-5	0.0	0.0	0.02789656406421703	0.0
2	2.6927185390170878E-5	0.0	0.0	0.12550761110358646	0.0
3	2.6927185390170878E-5	0.0	0.0	0.24089060050046868	0.0
4	2.6927185390170878E-5	0.0	0.0	0.34641824004454835	0.0
5	2.6927185390170878E-5	0.0	0.0	0.7036073542451651	0.0
6	2.6927185390170878E-5	0.0	0.0	1.3305260844991234	0.0
7	2.6927185390170878E-5	0.0	0.0	1.708502985820952	0.0
8	2.6927185390170878E-5	0.0	0.0	2.153501651578916	0.0
9	2.6927185390170878E-5	0.0	0.0	2.45829046301026	0.0
10	2.6927185390170878E-5	0.0	0.0	2.817122135519677	0.0
11	2.6927185390170878E-5	0.0	0.0	3.1361015736516413	0.0
12	2.6927185390170878E-5	0.0	0.0	3.373060805085145	0.0
13	2.6927185390170878E-5	0.0	0.0	3.5193562033099437	0.0
14	2.6927185390170878E-5	0.0	0.0	3.6202523669669135	0.0
15	2.6927185390170878E-5	0.0	0.0	3.6966717191042187	0.0
16	2.6927185390170878E-5	0.0	0.0	3.7912399941944988	0.0
17	2.6927185390170878E-5	0.0	0.0	3.9037417747546326	0.0
18	2.6927185390170878E-5	0.0	0.0	4.041258910542235	0.0
19	2.6927185390170878E-5	0.0	0.0	4.1389238119523855	0.0
20	2.6927185390170878E-5	0.0	0.0	4.242216495109081	0.0
21	2.6927185390170878E-5	0.0	0.0	4.364115863370384	0.0
22	2.6927185390170878E-5	0.0	0.0	4.501471436045646	0.0
23	2.6927185390170878E-5	0.0	0.0	4.649382465393854	0.0
24	2.6927185390170878E-5	0.0	0.0	4.772708974480837	0.0
25	2.6927185390170878E-5	0.0	0.0	4.879690682035986	0.0
26	2.6927185390170878E-5	0.0	0.0	4.984437433203751	0.0
27	2.6927185390170878E-5	0.0	0.0	5.098339427404174	0.0
28	2.6927185390170878E-5	0.0	0.0	5.223281567614566	0.0
29	2.6927185390170878E-5	0.0	0.0	5.360664067475218	0.0
30	2.6927185390170878E-5	0.0	0.0	5.523842810939653	0.0
31	8.078155617051264E-5	0.0	0.0	5.671807694658643	0.0
32	8.078155617051264E-5	0.0	0.0	5.819826432748412	0.0
33	8.078155617051264E-5	0.0	0.0	5.960467122041274	0.0
34	8.078155617051264E-5	0.0	0.0	6.097822694716537	0.0
35	8.078155617051264E-5	0.0	0.0	6.24963816594632	0.0
36	8.078155617051264E-5	0.0	0.0	6.392810010665858	0.0
37	8.078155617051264E-5	0.0	0.0	6.55695813280434	0.0
38	8.078155617051264E-5	0.0	0.0	6.738043454553239	0.0
39	1.0770874156068351E-4	0.0	0.0	6.987389191266221	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	46400	0.0	43.855606	1
GCGTAAG	230	0.0	43.04348	1
GTTTTTT	53605	0.0	38.47309	2
GGGCGAT	3975	0.0	38.37736	7
TCGACGG	135	0.0	38.333336	2
ACGGGAC	1215	0.0	37.592594	5
CGTAGTA	30	1.14041235E-4	37.500004	20
CGGTCTA	495	0.0	37.272728	31
CGACGGT	505	0.0	36.980198	28
GTAGGGA	5190	0.0	36.979767	4
GCGATCG	300	0.0	36.750004	9
TAGGGAC	3900	0.0	36.576927	5
AGGGCGA	2130	0.0	36.549297	6
AGGGATT	5415	0.0	36.44044	6
CGGGATC	960	0.0	36.32813	6
TAAGGGA	4935	0.0	36.291794	4
TATGGGA	4065	0.0	36.254616	4
TAGGGAG	4970	0.0	36.217304	5
CGAGGGA	2410	0.0	36.130707	4
AATGCGG	480	0.0	36.093754	2
>>END_MODULE