FastQCFastQC Report
Thu 26 May 2016
SRR1547361_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547361_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2225825
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT812123.648624667258207No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT34020.15284220457583145No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCT29270.13150180270236878No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC207.034712E-445.022
GTACGTA207.034712E-445.023
CGTTTTT326950.044.2430041
TCGATAG850.042.3529431
TATAGCG1350.041.6666641
CGGTCTA1850.041.35135331
CGACGGT2050.040.60975628
CGCATCG451.9297659E-840.00000421
TACGGGA9150.039.8360674
GTTTTTT366700.039.717072
TCGTGCG800.039.3751
GCGGGAT12800.038.4960945
TATCGCG1000.038.251
CATACGA2650.038.20754618
GCGCGAC2500.037.8000039
ATAGCGG4000.037.68752
AGGGATC22750.037.582426
CCAATCG900.037.50000424
TAAGGGA29200.037.448634
TAGGGAC22850.037.4179425
Produced by FastQC (version 0.11.5)