##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547361_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2225825
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32542899823661	31.0	31.0	33.0	30.0	34.0
2	31.713925847719384	31.0	31.0	34.0	30.0	34.0
3	31.594556175799983	31.0	31.0	34.0	30.0	34.0
4	35.4903835656442	37.0	35.0	37.0	33.0	37.0
5	35.434550335268945	37.0	35.0	37.0	33.0	37.0
6	35.48689587007065	37.0	35.0	37.0	33.0	37.0
7	35.939783675715745	37.0	35.0	37.0	35.0	37.0
8	35.94375793245201	37.0	35.0	37.0	35.0	37.0
9	37.66061437893815	39.0	37.0	39.0	35.0	39.0
10	37.0265209529051	39.0	37.0	39.0	33.0	39.0
11	36.71893522626442	39.0	37.0	39.0	32.0	39.0
12	36.49219772443925	39.0	35.0	39.0	32.0	39.0
13	36.405873777138815	39.0	35.0	39.0	32.0	39.0
14	37.46560174317388	40.0	36.0	41.0	32.0	41.0
15	37.58922017678838	40.0	36.0	41.0	32.0	41.0
16	37.63397796322712	40.0	36.0	41.0	32.0	41.0
17	37.55289791425651	40.0	36.0	41.0	32.0	41.0
18	37.28092909370683	39.0	36.0	40.0	32.0	41.0
19	37.05982141453169	38.0	36.0	40.0	32.0	41.0
20	36.72501971179226	38.0	35.0	40.0	31.0	41.0
21	36.60799119427628	38.0	35.0	40.0	31.0	41.0
22	36.576481978592206	38.0	35.0	40.0	31.0	41.0
23	36.497162175822446	38.0	35.0	40.0	31.0	41.0
24	36.331000864847866	38.0	35.0	40.0	31.0	41.0
25	36.24797726685611	38.0	35.0	40.0	31.0	41.0
26	36.10241281322656	38.0	35.0	40.0	30.0	41.0
27	36.00670942234902	38.0	35.0	40.0	30.0	41.0
28	35.97685487403547	38.0	35.0	40.0	30.0	41.0
29	35.90700976042591	38.0	35.0	40.0	30.0	41.0
30	35.74050116249031	38.0	35.0	40.0	30.0	41.0
31	35.50838408230656	38.0	34.0	40.0	28.0	41.0
32	35.20326440757921	38.0	34.0	40.0	26.0	41.0
33	34.72869834780362	37.0	34.0	40.0	23.0	41.0
34	34.22523468826166	37.0	33.0	40.0	21.0	41.0
35	33.86725461345793	37.0	33.0	40.0	18.0	41.0
36	33.6526753001696	37.0	33.0	40.0	18.0	41.0
37	33.534768456639675	37.0	33.0	40.0	16.0	41.0
38	33.41057001336583	37.0	33.0	40.0	15.0	41.0
39	33.35175137308639	37.0	33.0	40.0	15.0	41.0
40	33.208409466153	37.0	32.0	40.0	15.0	41.0
41	33.09082744600317	37.0	32.0	40.0	15.0	41.0
42	33.01016432109443	37.0	32.0	40.0	15.0	41.0
43	32.95683083800389	36.0	32.0	40.0	15.0	41.0
44	32.82350005054306	36.0	31.0	40.0	13.0	41.0
45	32.75000550357733	36.0	31.0	40.0	13.0	41.0
46	32.70459851965002	36.0	31.0	40.0	13.0	41.0
47	32.565494142621276	36.0	31.0	40.0	12.0	41.0
48	32.48561634450148	35.0	31.0	40.0	12.0	41.0
49	32.36699021711051	35.0	31.0	40.0	12.0	41.0
50	32.29062976649108	35.0	31.0	40.0	12.0	41.0
51	31.920466343939886	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	6.0
12	6.0
13	12.0
14	41.0
15	107.0
16	281.0
17	729.0
18	1632.0
19	3131.0
20	5230.0
21	8232.0
22	12966.0
23	18876.0
24	28195.0
25	43656.0
26	61906.0
27	69696.0
28	67478.0
29	64397.0
30	66435.0
31	74309.0
32	86361.0
33	106176.0
34	151669.0
35	196024.0
36	188769.0
37	211160.0
38	296843.0
39	461372.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.480271360057507	21.48569631484955	26.021003448159668	24.01302887693327
2	39.13915065200544	23.02067772623634	26.594049397414445	11.246122224343782
3	25.100266193433896	22.635966439410108	40.61109925533229	11.652668111823704
4	22.39987420394685	24.90505767524401	40.03886199499062	12.656206125818517
5	20.693855087439488	28.041602551862795	39.0190154212483	12.245526939449418
6	18.8856266777487	36.290229465479094	35.63725809531297	9.186885761459235
7	78.10582592971146	3.062504913908326	17.262722810643243	1.5689463457369741
8	79.09323509260612	2.451315804252356	16.959644176878236	1.4958049262632955
9	74.78840429952939	5.013421989599362	17.97221255040266	2.2259611604685903
10	34.67770377275842	32.32855233452765	23.677602686644278	9.31614120606966
11	24.222569159749753	24.39562858715308	35.16785910842047	16.213943144676694
12	23.608280075926906	21.82804128806173	37.849696180068065	16.713982455943302
13	22.230903148270865	22.837374905933753	37.74937382768187	17.18234811811351
14	16.769197937843273	26.50945155167185	39.30493187919086	17.416418631294015
15	17.080453315062954	26.865184819112013	40.61267170599665	15.441690159828378
16	21.37966821290982	25.715858164950074	38.29838374535285	14.606089876787259
17	21.105882088663755	25.86726270034706	35.982972605663065	17.043882605326115
18	20.762009591949052	26.69854997585165	37.025732031943214	15.513708400256085
19	21.820583379196478	28.15504363550594	34.54862803679535	15.475744948502241
20	21.52141340851145	28.70769265328586	34.86513989195018	14.905754046252511
21	21.650489144474523	26.256511630519018	37.678703402109335	14.414295822897127
22	20.64106567227882	23.803443666954948	38.269899924747	17.28559073601923
23	18.706142666202418	26.501229881055338	37.644648613435464	17.147978839306774
24	18.5568946345737	27.36324733525771	37.91416665730684	16.165691372861748
25	19.81939280940775	28.016353486909345	35.446542293306976	16.717711410375927
26	17.959228600631228	29.66360787573147	34.71090494535734	17.666258578279965
27	17.251625801669043	28.61680500488583	37.234778115979466	16.896791077465657
28	16.65876697404333	28.614109375175495	37.48565138768771	17.24147226309346
29	18.689654397807555	27.415228061505285	36.77989060236092	17.115226938326238
30	19.403277436456147	27.245628025563555	36.85797400963688	16.493120528343425
31	21.435467747913695	26.89169184459695	35.199397976031364	16.473442431458
32	21.904911661968036	27.862972156391454	34.156818258398566	16.075297923241944
33	21.431199667539	28.180157918973865	33.38744061190794	17.001201801579192
34	19.262251075443938	29.266676400885068	34.41959722799412	17.051475295676884
35	19.07872361933216	30.33517909089888	33.036739186593735	17.549358103175226
36	20.905372165376882	30.856064605258727	31.704469129423924	16.53409409994047
37	19.65491446991565	31.612682937787113	31.8649938786742	16.86740871362304
38	19.900486336526907	31.4107353453214	31.066593285635662	17.622185032516033
39	19.276987184527087	30.586771197196544	31.296126155470443	18.840115462805926
40	20.423842844787888	29.19847696921366	31.78129457616839	18.59638560983006
41	18.4257073220042	29.331955567036943	32.021205620387946	20.22113149057091
42	19.000954702189077	29.998315231431043	31.59493670886076	19.405793357519123
43	18.932216144575605	29.67479473902935	31.828378241775525	19.56461087461952
44	19.483202857367495	30.503970437927507	30.969236125930834	19.04359057877416
45	18.98922871294913	30.329967540125573	30.705648467422193	19.975155279503106
46	20.05849516471421	30.319140094122403	30.463940336729078	19.15842440443431
47	19.02741680051217	29.73706378533802	31.873035840643354	19.362483573506452
48	18.9483899228376	30.48501117563151	31.904889198387114	18.661709703143778
49	20.00233621241562	29.324632439657204	31.552974739703256	19.12005660822392
50	18.32960812283086	29.516112003414463	32.733076499724824	19.421203374029854
51	17.699909022497277	30.036817809126955	32.8489885772691	19.414284591106668
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2971.0
1	3591.5
2	4212.0
3	57346.0
4	110480.0
5	71531.0
6	32582.0
7	32059.0
8	31536.0
9	33314.0
10	35092.0
11	36494.0
12	37896.0
13	37519.5
14	37143.0
15	35633.5
16	34124.0
17	30888.5
18	27653.0
19	25592.0
20	23531.0
21	22409.0
22	21287.0
23	21422.5
24	21558.0
25	22696.5
26	26068.5
27	28302.0
28	32557.0
29	36812.0
30	42079.0
31	47346.0
32	51915.5
33	56485.0
34	62155.5
35	67826.0
36	75295.0
37	82764.0
38	89254.0
39	95744.0
40	104824.0
41	113904.0
42	123975.0
43	134046.0
44	143908.0
45	153770.0
46	154265.0
47	154760.0
48	151204.0
49	147648.0
50	138119.5
51	128591.0
52	120793.0
53	112995.0
54	106733.5
55	100472.0
56	96409.5
57	92347.0
58	82921.5
59	73496.0
60	63397.0
61	53298.0
62	45850.5
63	38403.0
64	31907.5
65	25412.0
66	20284.0
67	15156.0
68	12141.0
69	9126.0
70	7672.0
71	6218.0
72	4789.0
73	3360.0
74	2577.0
75	1324.0
76	854.0
77	684.5
78	515.0
79	373.5
80	232.0
81	195.0
82	158.0
83	96.5
84	35.0
85	29.0
86	23.0
87	19.5
88	16.0
89	12.0
90	8.0
91	5.0
92	2.0
93	3.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2225825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.590824190425508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.00796500277889	22.303120128473168
2	6.696592830178457	3.8292221656499246
3	3.050095264833707	2.616142124427295
4	1.9280875466414307	2.205024482790959
5	1.2871447624898391	1.8400264805986992
6	0.9696966588682321	1.6634656015046787
7	0.7561508955454163	1.513328412118026
8	0.5660812037028674	1.2947782538058503
9	0.48306201993213876	1.243002715645644
>10	5.201723778029459	33.8073486511066
>50	0.9335129942203365	18.186585617804173
>100	0.11574771930898034	4.479601395307888
>500	0.002547275981695992	0.55076719316355
>1k	0.0014328427397039955	0.7709830345292737
>5k	0.0	0.0
>10k+	1.592047488559995E-4	3.696603743074234
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81212	3.648624667258207	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT	3402	0.15284220457583145	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCT	2927	0.13150180270236878	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7970864735547494E-4	0.0	0.0	0.04578077791380724	0.0
2	1.7970864735547494E-4	0.0	0.0	0.135185829973156	0.0
3	1.7970864735547494E-4	0.0	0.0	0.21861556950793526	0.0
4	1.7970864735547494E-4	0.0	0.0	0.3251827973897319	0.0
5	1.7970864735547494E-4	0.0	0.0	0.6134803949097526	0.0
6	1.7970864735547494E-4	0.0	0.0	1.1259645300057282	0.0
7	1.7970864735547494E-4	0.0	0.0	1.5006570597418936	0.0
8	1.7970864735547494E-4	0.0	0.0	1.951186638662069	0.0
9	1.7970864735547494E-4	0.0	0.0	2.2222322060359643	0.0
10	1.7970864735547494E-4	0.0	0.0	2.5054081071063536	0.0
11	1.7970864735547494E-4	0.0	0.0	2.76409870497456	0.0
12	1.7970864735547494E-4	0.0	0.0	2.961194163961677	0.0
13	1.7970864735547494E-4	0.0	0.0	3.0767468242112477	0.0
14	1.7970864735547494E-4	0.0	0.0	3.1627374119708422	0.0
15	1.7970864735547494E-4	0.0	0.0	3.225096312603192	0.0
16	1.7970864735547494E-4	0.0	0.0	3.3162085968124178	0.0
17	1.7970864735547494E-4	0.0	0.0	3.4286612828951064	0.0
18	1.7970864735547494E-4	0.0	0.0	3.563577549897229	0.0
19	1.7970864735547494E-4	0.0	0.0	3.6551391057248437	0.0
20	1.7970864735547494E-4	0.0	0.0	3.757033908775398	0.0
21	1.7970864735547494E-4	0.0	0.0	3.870295283771186	0.0
22	1.7970864735547494E-4	0.0	0.0	3.998202913526445	0.0
23	1.7970864735547494E-4	0.0	0.0	4.132490200262824	0.0
24	1.7970864735547494E-4	0.0	0.0	4.246695045657228	0.0
25	1.7970864735547494E-4	0.0	0.0	4.3492637561353655	0.0
26	2.2463580919434366E-4	0.0	0.0	4.450125234463626	0.0
27	2.2463580919434366E-4	1.7970864735547494E-4	0.0	4.568643087394562	0.0
28	2.2463580919434366E-4	1.7970864735547494E-4	0.0	4.686307324250559	0.0
29	2.2463580919434366E-4	1.7970864735547494E-4	0.0	4.817359855334539	0.0
30	2.695629710332124E-4	1.7970864735547494E-4	0.0	4.972762908135186	0.0
31	2.695629710332124E-4	1.7970864735547494E-4	0.0	5.111677692540968	0.0
32	2.695629710332124E-4	1.7970864735547494E-4	0.0	5.249289589253423	0.0
33	2.695629710332124E-4	1.7970864735547494E-4	0.0	5.388114519335527	0.0
34	2.695629710332124E-4	1.7970864735547494E-4	0.0	5.535250974357822	0.0
35	2.695629710332124E-4	1.7970864735547494E-4	0.0	5.693439511192479	0.0
36	2.695629710332124E-4	1.7970864735547494E-4	0.0	5.846236788606472	0.0
37	2.695629710332124E-4	1.7970864735547494E-4	0.0	5.997596396841621	0.0
38	2.695629710332124E-4	1.7970864735547494E-4	0.0	6.194287511372188	0.0
39	2.695629710332124E-4	1.7970864735547494E-4	0.0	6.50954140599553	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATC	20	7.034712E-4	45.0	22
GTACGTA	20	7.034712E-4	45.0	23
CGTTTTT	32695	0.0	44.243004	1
TCGATAG	85	0.0	42.352943	1
TATAGCG	135	0.0	41.666664	1
CGGTCTA	185	0.0	41.351353	31
CGACGGT	205	0.0	40.609756	28
CGCATCG	45	1.9297659E-8	40.000004	21
TACGGGA	915	0.0	39.836067	4
GTTTTTT	36670	0.0	39.71707	2
TCGTGCG	80	0.0	39.375	1
GCGGGAT	1280	0.0	38.496094	5
TATCGCG	100	0.0	38.25	1
CATACGA	265	0.0	38.207546	18
GCGCGAC	250	0.0	37.800003	9
ATAGCGG	400	0.0	37.6875	2
AGGGATC	2275	0.0	37.58242	6
CCAATCG	90	0.0	37.500004	24
TAAGGGA	2920	0.0	37.44863	4
TAGGGAC	2285	0.0	37.417942	5
>>END_MODULE