FastQCFastQC Report
Thu 26 May 2016
SRR1547360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547360_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2210204
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203360.9200960635307872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC207.0347026E-445.033
CGTTTTT89500.042.812851
CGGTCTA3600.042.531
TAGTACG900.042.51
TCACGAC3500.042.4285725
ATATGCG1300.041.5384641
ATACGGG6650.040.263163
CGACGGT3700.040.13513628
AATCCCG451.9297659E-840.016
CACGACG3750.039.626
TACGGGA10850.039.193554
GTAGGGA34500.038.6086964
GGGCGAT24900.038.584347
CGTTTGG3500.038.5714262
TAGGGAT44550.038.4343455
AGGGCGA12600.038.3928576
ACGGGAT11500.038.1521725
TAGGGAC29200.038.142125
ATAGGGA46100.038.0206074
TACGCGG1550.037.7419362
Produced by FastQC (version 0.11.5)