##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547360_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2210204
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.325388968620093	31.0	31.0	33.0	30.0	34.0
2	31.7451864171814	31.0	31.0	34.0	30.0	34.0
3	31.86414421474217	31.0	31.0	34.0	30.0	34.0
4	35.64145255370092	37.0	35.0	37.0	33.0	37.0
5	35.51576053613151	37.0	35.0	37.0	33.0	37.0
6	35.55034286427859	37.0	35.0	37.0	33.0	37.0
7	35.94190355279422	37.0	35.0	37.0	35.0	37.0
8	35.91965130820503	37.0	35.0	37.0	35.0	37.0
9	37.60943424226904	39.0	37.0	39.0	35.0	39.0
10	36.95701256535596	39.0	37.0	39.0	32.0	39.0
11	36.723939962103046	39.0	37.0	39.0	32.0	39.0
12	36.425502351819105	39.0	35.0	39.0	32.0	39.0
13	36.29465243932234	39.0	35.0	39.0	32.0	39.0
14	37.42124301648174	39.0	36.0	41.0	32.0	41.0
15	37.55486778596003	40.0	36.0	41.0	32.0	41.0
16	37.59668881243541	40.0	36.0	41.0	32.0	41.0
17	37.50509229012344	39.0	36.0	41.0	32.0	41.0
18	37.384189875685685	39.0	36.0	41.0	32.0	41.0
19	37.33894382599977	39.0	36.0	41.0	32.0	41.0
20	37.185966544264694	39.0	35.0	41.0	32.0	41.0
21	37.07270279123556	39.0	35.0	41.0	31.0	41.0
22	36.97595425580625	39.0	35.0	41.0	31.0	41.0
23	36.91057658026137	39.0	35.0	41.0	31.0	41.0
24	36.78150161704531	39.0	35.0	41.0	31.0	41.0
25	36.70783556630972	38.0	35.0	40.0	31.0	41.0
26	36.59427591299265	38.0	35.0	40.0	31.0	41.0
27	36.499460683267245	38.0	35.0	40.0	30.0	41.0
28	36.56597264324922	38.0	35.0	40.0	30.0	41.0
29	36.57933159111105	38.0	35.0	41.0	30.0	41.0
30	36.52318654748611	38.0	35.0	41.0	30.0	41.0
31	36.424590671268355	38.0	35.0	41.0	30.0	41.0
32	36.325117048019095	38.0	35.0	41.0	30.0	41.0
33	36.144068149365395	38.0	35.0	41.0	30.0	41.0
34	35.94922369156874	38.0	35.0	41.0	29.0	41.0
35	35.803376520900336	38.0	35.0	40.0	28.0	41.0
36	35.67046978468956	38.0	35.0	40.0	27.0	41.0
37	35.59213493415088	38.0	35.0	40.0	27.0	41.0
38	35.51331234582871	38.0	34.0	40.0	27.0	41.0
39	35.47627277844036	38.0	34.0	40.0	27.0	41.0
40	35.32853845165424	38.0	34.0	40.0	26.0	41.0
41	35.24506516140592	38.0	34.0	40.0	25.0	41.0
42	35.16585844564574	38.0	34.0	40.0	25.0	41.0
43	35.11380487954958	38.0	34.0	40.0	25.0	41.0
44	34.998596509643455	38.0	34.0	40.0	25.0	41.0
45	34.908013920886944	38.0	34.0	40.0	24.0	41.0
46	34.8252754044423	38.0	34.0	40.0	24.0	41.0
47	34.69408977632834	37.0	33.0	40.0	24.0	41.0
48	34.63620869385812	37.0	33.0	40.0	24.0	41.0
49	34.52313768321838	37.0	33.0	40.0	24.0	41.0
50	34.46430691465584	37.0	33.0	40.0	24.0	41.0
51	34.02545285412568	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	1.0
12	5.0
13	11.0
14	27.0
15	60.0
16	193.0
17	471.0
18	1071.0
19	1978.0
20	3332.0
21	5429.0
22	7949.0
23	11726.0
24	16715.0
25	23581.0
26	31887.0
27	38489.0
28	42490.0
29	47600.0
30	55426.0
31	67078.0
32	82242.0
33	104303.0
34	152789.0
35	200384.0
36	181035.0
37	226900.0
38	337660.0
39	569199.0
40	170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.82289779585957	24.15519110453153	27.312094268221394	15.70981683138751
2	30.69920242656334	25.757848596781113	29.6385311039162	13.90441787273935
3	28.177308519937526	25.932040662309902	32.30104551435071	13.589605303401859
4	26.040039742937754	27.552615052728164	32.0110722811107	14.396272923223377
5	23.58298148044253	31.914701086415555	30.232186712176794	14.270130720965124
6	22.28540894867623	40.382335748193384	26.908104410271633	10.424150892858759
7	88.61521379926921	3.114373152885435	6.827062117343015	1.4433509305023426
8	89.028252595688	2.6878061934554456	6.776252327839422	1.5076888830171333
9	83.3895423227901	6.497906980532115	7.895470282381174	2.217080414296599
10	34.41134845471277	42.625748573434855	14.130234132233948	8.832668839618425
11	29.05736303074286	22.64655208297515	30.192326138220725	18.103758748061267
12	28.17233160377956	21.774596372099587	31.63033819502634	18.422733829094508
13	23.635329589485856	25.55062790584037	32.05898641030421	18.755056094369568
14	18.557789235744753	29.94085613816643	31.544644747724643	19.95670987836417
15	17.77528228163554	28.95103800373178	35.60273169354503	17.670948021087646
16	22.534842937574993	27.249158901169302	33.77014067479744	16.445857486458262
17	21.976070987112504	27.15337588747464	29.750964164393874	21.119588961018984
18	22.118727502076734	27.859690779674633	31.67191806729153	18.34966365095711
19	23.98570448700663	28.550577231784942	28.96090134666302	18.502816934545407
20	24.92050507554959	29.094011231542428	29.06247568097786	16.923008011930122
21	25.463486628383624	26.005925244909523	31.813262486177745	16.71732564052911
22	23.878384076763957	23.737130147262427	31.928545962273162	20.455939813700454
23	21.354589893059643	26.478460811762172	31.790278182466412	20.376671112711765
24	20.282109705710425	27.350642746099457	33.94858574140668	18.418661806783447
25	20.529960130377106	29.30679701964163	30.701509905872943	19.461732944108327
26	20.0192832878775	31.504693684383884	28.289832069799893	20.186190957938724
27	18.969063489162085	30.353894934585224	31.877147991769085	18.799893584483605
28	18.24664148648722	29.683051881183818	33.27339919754014	18.796907434788825
29	19.038830804758295	27.59315429706941	33.4622957880811	19.905719110091194
30	19.899158629701148	28.461988124173153	32.79896335360899	18.83988989251671
31	22.58701006784894	28.409232812898722	29.571433225168352	19.432323894083986
32	23.050768164386636	28.287388856413255	29.593784103186856	19.068058876013254
33	21.916438482601606	28.27960676932989	29.539897674603793	20.264057073464713
34	20.182209425012353	28.32448950413627	30.616540373648764	20.876760697202613
35	20.616920429064468	28.15640547207407	30.636764751127043	20.589909347734416
36	23.043529013611412	28.451943802472535	28.95144520596289	19.553081977953166
37	20.624250069224377	29.833807196077828	29.652330735081467	19.889611999616324
38	20.712431974605057	30.50727444163525	28.3549844267769	20.42530915698279
39	20.368708046859023	29.799421229895522	28.77974159851308	21.052129124732378
40	21.694106064417586	28.618625249072032	29.196626193781206	20.490642492729176
41	20.10633407594955	28.062613224842593	29.75983212409352	22.071220575114335
42	20.677910274345717	29.07831132329866	28.542568921239848	21.70120948111577
43	20.42300167767319	28.40389393920199	29.079487685299636	22.093616697825176
44	19.96413000790877	28.859960438041014	29.837064813926677	21.338844740123537
45	20.283014599557326	30.091204250829335	27.872133070069548	21.75364807954379
46	21.442274106824527	29.9309023058505	28.822090630548132	19.804732956776842
47	21.105065414776192	28.53076005653777	29.392716690405045	20.97145783828099
48	20.66863511241496	28.24336577076143	30.70834185441706	20.379657262406546
49	20.546745911237153	28.112970567422735	30.677801687084088	20.662481834256024
50	20.0453442306683	29.044875495655603	30.33267517387535	20.577105099800743
51	19.364094898027513	29.4546114295332	29.484291947711615	21.69700172472767
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2821.0
1	3516.5
2	4212.0
3	17915.0
4	31618.0
5	21463.5
6	11309.0
7	11569.5
8	11830.0
9	12640.0
10	13450.0
11	13753.0
12	14056.0
13	13496.0
14	12936.0
15	12460.5
16	11985.0
17	11193.0
18	10401.0
19	10897.0
20	11393.0
21	12074.5
22	12756.0
23	15245.5
24	17735.0
25	21881.0
26	30103.5
27	34180.0
28	40661.5
29	47143.0
30	52554.0
31	57965.0
32	66531.5
33	75098.0
34	85148.5
35	95199.0
36	99448.0
37	103697.0
38	108440.5
39	113184.0
40	121088.0
41	128992.0
42	142790.0
43	156588.0
44	160336.0
45	164084.0
46	163915.0
47	163746.0
48	157705.5
49	151665.0
50	140484.0
51	129303.0
52	121402.5
53	113502.0
54	107552.0
55	101602.0
56	94725.5
57	87849.0
58	84279.0
59	80709.0
60	71971.0
61	63233.0
62	55688.0
63	48143.0
64	41641.0
65	35139.0
66	28683.0
67	22227.0
68	19359.0
69	16491.0
70	14232.0
71	11973.0
72	9893.5
73	7814.0
74	6086.0
75	3086.5
76	1815.0
77	1418.5
78	1022.0
79	739.0
80	456.0
81	362.5
82	269.0
83	209.5
84	150.0
85	92.5
86	35.0
87	28.5
88	22.0
89	19.5
90	17.0
91	10.5
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2210204.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.854201633856718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.57777543425125	16.196616023131075
2	6.166060015935879	2.4484439768570545
3	2.059202317216167	1.226514540327443
4	1.0944860770474143	0.869205890365928
5	0.7578740953469638	0.7523492551047686
6	0.5260532267330926	0.626662010021986
7	0.4071672064197442	0.5658785870466232
8	0.3212393743659147	0.5102361049115883
9	0.2602407742957731	0.4650185525597348
>10	3.8169187551240347	19.25284639696418
>50	1.9715302196911606	28.368046285163135
>100	1.037552791628406	27.170443289127803
>500	0.0027527378429807858	0.3470491673410209
>1k	9.175792809935953E-4	0.2744967626724847
>5k	0.0	0.0
>10k+	2.2939482024839883E-4	0.9261931584051182
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20336	0.9200960635307872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.048938469028198E-5	0.0	0.0	0.01506648255093195	0.0
2	9.048938469028198E-5	0.0	0.0	0.05230286435098298	0.0
3	9.048938469028198E-5	0.0	0.0	0.07922345629634188	0.0
4	9.048938469028198E-5	0.0	0.0	0.12632318102763365	0.0
5	9.048938469028198E-5	0.0	0.0	0.23509142142535258	0.0
6	9.048938469028198E-5	0.0	0.0	0.3900544927074605	0.0
7	9.048938469028198E-5	0.0	0.0	0.4790055578580077	0.0
8	9.048938469028198E-5	0.0	0.0	0.6758652142517161	0.0
9	9.048938469028198E-5	0.0	0.0	0.7559030750102705	0.0
10	9.048938469028198E-5	0.0	0.0	0.8860720548872412	0.0
11	9.048938469028198E-5	0.0	0.0	1.046781202097182	0.0
12	9.048938469028198E-5	0.0	0.0	1.183510662364198	0.0
13	9.048938469028198E-5	0.0	0.0	1.2462650506469086	0.0
14	9.048938469028198E-5	0.0	0.0	1.2761717922870468	0.0
15	9.048938469028198E-5	0.0	0.0	1.3089289495449288	0.0
16	9.048938469028198E-5	0.0	0.0	1.3751671791382154	0.0
17	9.048938469028198E-5	0.0	0.0	1.461448807440399	0.0
18	9.048938469028198E-5	0.0	0.0	1.5609418859073643	0.0
19	9.048938469028198E-5	0.0	0.0	1.6297590629643237	0.0
20	9.048938469028198E-5	0.0	0.0	1.7069465081051343	0.0
21	9.048938469028198E-5	0.0	0.0	1.8013721810294434	0.0
22	9.048938469028198E-5	0.0	0.0	1.9141219543535348	0.0
23	9.048938469028198E-5	0.0	0.0	2.0272789299087326	0.0
24	9.048938469028198E-5	0.0	0.0	2.1166371972903857	0.0
25	9.048938469028198E-5	0.0	0.0	2.1928292591996033	0.0
26	9.048938469028198E-5	0.0	0.0	2.2740434819591315	0.0
27	9.048938469028198E-5	0.0	0.0	2.3540813427176857	0.0
28	9.048938469028198E-5	0.0	0.0	2.441675067097879	0.0
29	9.048938469028198E-5	0.0	0.0	2.533748016020241	0.0
30	9.048938469028198E-5	0.0	0.0	2.6507055457324302	0.0
31	1.3573407703542297E-4	0.0	0.0	2.755492253203777	0.0
32	1.3573407703542297E-4	0.0	0.0	2.856342672441096	0.0
33	1.3573407703542297E-4	0.0	0.0	2.9636178379914253	0.0
34	1.3573407703542297E-4	0.0	0.0	3.0724313230814895	0.0
35	1.3573407703542297E-4	0.0	0.0	3.1970352058000078	0.0
36	1.3573407703542297E-4	0.0	0.0	3.3161192360524185	0.0
37	1.3573407703542297E-4	0.0	0.0	3.4377369690761577	0.0
38	1.3573407703542297E-4	0.0	0.0	3.558811765791755	0.0
39	1.3573407703542297E-4	0.0	0.0	3.728569851470724	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	20	7.0347026E-4	45.0	33
CGTTTTT	8950	0.0	42.81285	1
CGGTCTA	360	0.0	42.5	31
TAGTACG	90	0.0	42.5	1
TCACGAC	350	0.0	42.42857	25
ATATGCG	130	0.0	41.538464	1
ATACGGG	665	0.0	40.26316	3
CGACGGT	370	0.0	40.135136	28
AATCCCG	45	1.9297659E-8	40.0	16
CACGACG	375	0.0	39.6	26
TACGGGA	1085	0.0	39.19355	4
GTAGGGA	3450	0.0	38.608696	4
GGGCGAT	2490	0.0	38.58434	7
CGTTTGG	350	0.0	38.571426	2
TAGGGAT	4455	0.0	38.434345	5
AGGGCGA	1260	0.0	38.392857	6
ACGGGAT	1150	0.0	38.152172	5
TAGGGAC	2920	0.0	38.14212	5
ATAGGGA	4610	0.0	38.020607	4
TACGCGG	155	0.0	37.741936	2
>>END_MODULE