FastQCFastQC Report
Thu 26 May 2016
SRR1547359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547359_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences361289
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24620.6814489231612365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG207.0268573E-445.01
ACGTTAG351.2088276E-745.01
CACGACG502.1827873E-1145.026
TCGTAAG207.0268573E-445.01
CGGTCTA551.8189894E-1245.031
TGCACGG207.0268573E-445.02
TGTAGCG302.1613541E-644.9999961
TAAGCGG650.041.538462
CGTTTTT12300.041.524391
AGGGTAT2200.040.9090926
AGGGCGA2200.040.9090926
ACAACGA501.0768417E-940.513
AATGCGG451.9219442E-840.02
ACGGGAC1300.039.807695
CGGGTAT403.4494406E-739.3756
CGACGTT403.4494406E-739.37527
TTTCGAC403.4494406E-739.37524
TACGGGA2000.039.3754
ATAGGGA6700.039.2910464
GGGCGAT3850.039.1558467
Produced by FastQC (version 0.11.5)