FastQCFastQC Report
Thu 26 May 2016
SRR1547358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547358_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2399703
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448141.8674811007862224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26830.11180550259761311No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA207.0348236E-445.041
CCGTACA207.0348236E-445.012
CGGTCCA253.8920793E-545.044
ACGCGTC207.0348236E-445.034
CGTTTTT173050.043.5567781
CGACGGT4300.042.90697528
TACGTAG2000.041.6251
CTACGCG603.6379788E-1241.2500041
TACGCGG1700.041.029412
CGGTCTA4450.040.95505531
AGGGCGA13600.040.533096
TCTCGCG1400.040.17857432
GGCGATA5100.040.147068
CGAAACG451.9297659E-840.00000437
CACGACG4500.040.026
CTACGGG4050.040.03
ATAGGGA35200.039.7585224
CGCATCG1700.039.70588321
TCACGAC4550.039.5604425
TTAGGGA35700.039.5168084
Produced by FastQC (version 0.11.5)