##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547358_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2399703
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.357910124711267	31.0	31.0	33.0	30.0	34.0
2	31.763377801336247	31.0	31.0	34.0	30.0	34.0
3	31.80583680563803	31.0	31.0	34.0	30.0	34.0
4	35.61839277610604	37.0	35.0	37.0	33.0	37.0
5	35.51960471775049	37.0	35.0	37.0	33.0	37.0
6	35.56468112928975	37.0	35.0	37.0	33.0	37.0
7	35.976183719401945	37.0	35.0	37.0	35.0	37.0
8	35.976423332387384	37.0	35.0	37.0	35.0	37.0
9	37.709531554529875	39.0	38.0	39.0	35.0	39.0
10	37.05166014294269	39.0	37.0	39.0	33.0	39.0
11	36.703866270117594	39.0	37.0	39.0	32.0	39.0
12	36.50842791795485	39.0	35.0	39.0	32.0	39.0
13	36.40602732921533	39.0	35.0	39.0	32.0	39.0
14	37.52981264764848	40.0	36.0	41.0	32.0	41.0
15	37.674791005386915	40.0	36.0	41.0	32.0	41.0
16	37.723629549156705	40.0	36.0	41.0	32.0	41.0
17	37.63064929284999	40.0	36.0	41.0	32.0	41.0
18	37.45986232462934	39.0	36.0	41.0	32.0	41.0
19	37.364590951463576	39.0	36.0	41.0	32.0	41.0
20	37.173051831830854	39.0	35.0	41.0	32.0	41.0
21	37.054227960710136	39.0	35.0	41.0	32.0	41.0
22	36.98419887794448	39.0	35.0	41.0	32.0	41.0
23	36.89012056908709	38.0	35.0	41.0	31.0	41.0
24	36.781277933144224	38.0	35.0	40.0	31.0	41.0
25	36.70052377315026	38.0	35.0	40.0	31.0	41.0
26	36.574386080277435	38.0	35.0	40.0	31.0	41.0
27	36.49817373233271	38.0	35.0	40.0	31.0	41.0
28	36.53495370052044	38.0	35.0	40.0	31.0	41.0
29	36.53537000203775	38.0	35.0	40.0	31.0	41.0
30	36.474577062244784	38.0	35.0	40.0	30.0	41.0
31	36.35241236102968	38.0	35.0	40.0	30.0	41.0
32	36.19996224532786	38.0	35.0	41.0	30.0	41.0
33	35.936147514921636	38.0	35.0	40.0	29.0	41.0
34	35.673367079176046	38.0	35.0	40.0	27.0	41.0
35	35.488500451930925	38.0	35.0	40.0	26.0	41.0
36	35.33109305609903	38.0	34.0	40.0	25.0	41.0
37	35.251170665703214	38.0	34.0	40.0	25.0	41.0
38	35.164919158745896	38.0	34.0	40.0	25.0	41.0
39	35.12391075062206	38.0	34.0	40.0	24.0	41.0
40	34.98712965729509	38.0	34.0	40.0	24.0	41.0
41	34.899055008057246	38.0	34.0	40.0	24.0	41.0
42	34.839009660778856	38.0	34.0	40.0	23.0	41.0
43	34.78403410755414	38.0	34.0	40.0	23.0	41.0
44	34.66729841151176	38.0	34.0	40.0	23.0	41.0
45	34.58294005549853	38.0	34.0	40.0	23.0	41.0
46	34.514512004193854	38.0	33.0	40.0	23.0	41.0
47	34.392127275750376	37.0	33.0	40.0	23.0	41.0
48	34.3359107356202	37.0	33.0	40.0	23.0	41.0
49	34.21829909784669	37.0	33.0	40.0	23.0	41.0
50	34.163551489496825	37.0	33.0	40.0	23.0	41.0
51	33.75754958009387	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	2.0
13	9.0
14	16.0
15	81.0
16	181.0
17	458.0
18	957.0
19	1967.0
20	3421.0
21	5652.0
22	8538.0
23	12979.0
24	19334.0
25	28758.0
26	40606.0
27	48270.0
28	50859.0
29	53373.0
30	60199.0
31	72166.0
32	88360.0
33	111987.0
34	165900.0
35	218809.0
36	199622.0
37	242233.0
38	358472.0
39	606333.0
40	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.923951839040082	23.024807653280426	28.742181845003316	17.309058662676172
2	32.8095185112491	25.573664741011697	29.124729185236674	12.492087562502526
3	26.955919128325462	25.068935614115578	34.96536863103476	13.0097766265242
4	24.548537881562844	26.814359943709697	34.850521085317645	13.786581089409813
5	22.1663680880509	31.383925427438314	33.39059041889767	13.059116065613118
6	20.19795783061487	40.12963270871437	29.657045059326094	10.015364401344666
7	86.51791492530533	2.784011188051188	9.533221402815265	1.164852483828207
8	87.41385913173421	2.104843807754543	9.266771763005673	1.2145252975055663
9	83.01023084940094	5.005869476347698	10.284105991449776	1.69979368280158
10	37.98470060670008	37.134262031593074	16.698816478539218	8.182220883167625
11	24.510574850304394	25.34734506728541	32.5960337591777	17.5460463232325
12	26.81365152270927	22.357058352637807	32.93361720179539	17.895672922857536
13	22.58196118436323	25.74710287064691	32.7672632821645	18.903672662825358
14	16.8042878639565	30.363340796756933	33.253156744813836	19.57921459447273
15	16.589469613531342	29.540238937901897	36.72792008010992	17.142371368456846
16	21.22646010777167	27.38159680593807	35.651161831276625	15.740781255013642
17	20.84016230341838	27.216701400131598	31.785558462859782	20.15757783359024
18	20.781655063147397	28.58962129896908	32.90936420048648	17.719359437397046
19	22.380644604769838	29.916285473660697	30.015006023662096	17.688063897907366
20	24.04526726849114	29.41922396229867	30.746388198872943	15.789120570337245
21	23.800070258694515	26.98496438934318	33.19910838966322	16.015856962299086
22	22.544623230458104	24.629964624788983	33.49497833690253	19.33043380785039
23	19.59238289071606	27.70480346942934	33.298829063429935	19.403984576424666
24	18.883128453812827	28.17198628330256	35.2658224788651	17.67906278401952
25	20.126282294100562	29.385511457042806	32.30333087052856	18.18487537832807
26	19.081236302992497	31.88052854874124	29.806855265005712	19.231379883260555
27	18.377107500386504	30.78801835060422	33.25519866416802	17.579675484841246
28	17.0677788042937	29.950039650740113	34.86377272520807	18.118408819758113
29	17.859001718129285	28.55970092965671	34.70871186976055	18.872585482453452
30	18.789366850814453	28.97833606908855	34.14518379982856	18.087113280268433
31	21.276799670625905	28.502485515915925	31.48568802055921	18.735026792898953
32	21.507869932237448	28.862780102370998	31.464685421487577	18.164664543903974
33	20.947467249072073	29.088474698743973	30.865652957886873	19.098405094297085
34	18.9255920420152	29.029217365649	31.866776846968147	20.178413745367656
35	19.289053687060438	29.07455630967666	31.373174096961165	20.26321590630174
36	21.09840259398767	29.71438548853754	30.3557148530464	18.83149706442839
37	19.495329213656856	31.395843568974996	30.68508894642379	18.423738270944362
38	19.57667261323589	30.82339772880227	29.73863848984645	19.86129116811539
39	19.112781873423504	29.899616744238767	30.3332954119739	20.654305970363833
40	20.711771415045945	28.51648724863035	31.233781847170256	19.53795948915345
41	18.4197377758831	29.057929252078278	31.29420599132476	21.228126980713864
42	19.504830389427358	30.642833717339187	29.441435044253396	20.410900848980063
43	19.463741971402293	30.145147128623833	29.96837525310424	20.422735646869633
44	19.61359384890547	30.570908149883547	29.604538561647004	20.21095943956398
45	19.239422545206637	30.875737539187142	28.81198214945766	21.072857766148562
46	19.93409184386568	31.00271158555871	29.373593315506124	19.689603255069482
47	19.585882086241504	30.11476836925236	30.424098315499876	19.875251229006256
48	19.777197428181736	29.69825849282182	30.675629442476836	19.848914636519602
49	19.67776845717991	29.068680582555423	30.62241452379732	20.631136436467344
50	18.563047177088166	29.847151918383236	31.571031915199505	20.018768989329097
51	18.206294695635254	30.392511073245316	30.189986010768834	21.211208220350603
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3570.0
1	4165.5
2	4761.0
3	33535.5
4	62310.0
5	40544.0
6	18778.0
7	18909.5
8	19041.0
9	20730.5
10	22420.0
11	23104.5
12	23789.0
13	23776.5
14	23764.0
15	22656.5
16	21549.0
17	20445.0
18	19341.0
19	17948.5
20	16556.0
21	17265.5
22	17975.0
23	19565.0
24	21155.0
25	24487.5
26	31904.0
27	35988.0
28	43949.0
29	51910.0
30	55924.0
31	59938.0
32	68825.5
33	77713.0
34	85793.0
35	93873.0
36	102274.5
37	110676.0
38	116607.5
39	122539.0
40	131526.5
41	140514.0
42	149913.5
43	159313.0
44	159233.5
45	159154.0
46	162861.5
47	166569.0
48	172555.5
49	178542.0
50	170420.0
51	162298.0
52	150222.0
53	138146.0
54	123820.5
55	109495.0
56	101368.0
57	93241.0
58	85682.0
59	78123.0
60	69792.5
61	61462.0
62	52074.5
63	42687.0
64	34204.0
65	25721.0
66	19417.0
67	13113.0
68	9551.5
69	5990.0
70	5167.5
71	4345.0
72	3555.0
73	2765.0
74	2123.5
75	1068.5
76	655.0
77	512.0
78	369.0
79	260.0
80	151.0
81	99.0
82	47.0
83	34.5
84	22.0
85	18.5
86	15.0
87	11.5
88	8.0
89	6.0
90	4.0
91	3.0
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2399703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.680566816483577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.96556390096066	16.53733187271604
2	6.851174960612541	2.833723630887338
3	2.6009621066939026	1.6136811190387543
4	1.4757734983602255	1.2207932975533744
5	1.027982681111065	1.0629632261452653
6	0.7368189695635479	0.914270035902692
7	0.5585281742400688	0.8085475458382227
8	0.42213679231299667	0.6984022511299983
9	0.3371049228635208	0.6274368793300125
>10	3.5740425698274763	17.382287186496452
>50	1.358287292850619	20.402524494791177
>100	1.084322298631831	32.784545922601566
>500	0.0060848599763047	0.84395588952513
>1k	0.0010141433293841165	0.38976332631548255
>5k	0.0	0.0
>10k+	2.0282866587682333E-4	1.8797733217285875
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44814	1.8674811007862224	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2683	0.11180550259761311	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.167182355483158E-5	0.0	0.0	0.01716879130459061	0.0
2	4.167182355483158E-5	0.0	0.0	0.06959194533656873	0.0
3	4.167182355483158E-5	0.0	0.0	0.11830630707216684	0.0
4	4.167182355483158E-5	0.0	0.0	0.18510624023056185	0.0
5	4.167182355483158E-5	0.0	0.0	0.36362833233946035	0.0
6	4.167182355483158E-5	0.0	0.0	0.6339117799160979	0.0
7	4.167182355483158E-5	0.0	0.0	0.8113087327890159	0.0
8	4.167182355483158E-5	0.0	0.0	1.0903432633121681	0.0
9	4.167182355483158E-5	0.0	0.0	1.223734770511184	0.0
10	4.167182355483158E-5	0.0	0.0	1.396214448204632	0.0
11	4.167182355483158E-5	0.0	0.0	1.6145748036319494	0.0
12	4.167182355483158E-5	0.0	0.0	1.7778866801433344	0.0
13	4.167182355483158E-5	0.0	0.0	1.8511040741291735	0.0
14	4.167182355483158E-5	0.0	0.0	1.8909423374475924	0.0
15	4.167182355483158E-5	0.0	0.0	1.9339476593561786	0.0
16	4.167182355483158E-5	0.0	0.0	2.014249263346339	0.0
17	4.167182355483158E-5	0.0	0.0	2.1150950763490317	0.0
18	4.167182355483158E-5	0.0	0.0	2.225608752416445	0.0
19	4.167182355483158E-5	0.0	0.0	2.3043684989350766	0.0
20	4.167182355483158E-5	0.0	0.0	2.3907958609877973	0.0
21	4.167182355483158E-5	0.0	0.0	2.495100435345541	0.0
22	4.167182355483158E-5	0.0	0.0	2.625408227601499	0.0
23	4.167182355483158E-5	0.0	0.0	2.7470482805580523	0.0
24	4.167182355483158E-5	0.0	0.0	2.851769573151344	0.0
25	4.167182355483158E-5	0.0	0.0	2.9417807120297805	0.0
26	8.334364710966315E-5	0.0	0.0	3.028749807788714	0.0
27	8.334364710966315E-5	0.0	0.0	3.127595373260774	0.0
28	8.334364710966315E-5	0.0	0.0	3.2223571000244613	0.0
29	8.334364710966315E-5	0.0	0.0	3.339163221448654	0.0
30	8.334364710966315E-5	0.0	0.0	3.481430827064849	0.0
31	8.334364710966315E-5	0.0	0.0	3.6009039451965514	0.0
32	8.334364710966315E-5	0.0	0.0	3.713834587030145	0.0
33	8.334364710966315E-5	0.0	0.0	3.8303906775130088	0.0
34	8.334364710966315E-5	0.0	0.0	3.9563229282957098	0.0
35	8.334364710966315E-5	0.0	0.0	4.101465889737188	0.0
36	8.334364710966315E-5	0.0	0.0	4.233232195817566	0.0
37	8.334364710966315E-5	0.0	0.0	4.368415591429439	0.0
38	8.334364710966315E-5	0.0	0.0	4.515933846813543	0.0
39	8.334364710966315E-5	0.0	0.0	4.743545347070033	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCGTA	20	7.0348236E-4	45.0	41
CCGTACA	20	7.0348236E-4	45.0	12
CGGTCCA	25	3.8920793E-5	45.0	44
ACGCGTC	20	7.0348236E-4	45.0	34
CGTTTTT	17305	0.0	43.556778	1
CGACGGT	430	0.0	42.906975	28
TACGTAG	200	0.0	41.625	1
CTACGCG	60	3.6379788E-12	41.250004	1
TACGCGG	170	0.0	41.02941	2
CGGTCTA	445	0.0	40.955055	31
AGGGCGA	1360	0.0	40.53309	6
TCTCGCG	140	0.0	40.178574	32
GGCGATA	510	0.0	40.14706	8
CGAAACG	45	1.9297659E-8	40.000004	37
CACGACG	450	0.0	40.0	26
CTACGGG	405	0.0	40.0	3
ATAGGGA	3520	0.0	39.758522	4
CGCATCG	170	0.0	39.705883	21
TCACGAC	455	0.0	39.56044	25
TTAGGGA	3570	0.0	39.516808	4
>>END_MODULE