FastQCFastQC Report
Thu 26 May 2016
SRR1547357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547357_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1518696
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108800.7164040729678619No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG207.034004E-445.01
CGTTATA207.034004E-445.038
CGCTATT207.034004E-445.07
TATACGA207.034004E-445.013
TAGTACG253.8914E-545.01
CTATGCG650.044.9999961
TCCGATT302.1659653E-644.99999613
ACTCGAT600.044.99999620
GCGATAA1150.043.043489
ACGGGTA900.042.55
CGTTTTT61950.042.167071
TACGAAA2150.041.86046620
ATACGAG700.041.7857131
CGACGGT3750.041.428
CGGTCTA3700.041.35135331
TCGAATA501.0822987E-940.538
CACGACG3850.040.32467326
TCTAGCG900.040.01
GGGCGAT16350.039.7706457
CGTAAGG1650.039.5454562
Produced by FastQC (version 0.11.5)