##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547357_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1518696
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.316941639406437	31.0	31.0	33.0	30.0	34.0
2	31.732422420286877	31.0	31.0	34.0	30.0	34.0
3	31.844348045955215	31.0	31.0	34.0	30.0	34.0
4	35.61618454252859	37.0	35.0	37.0	33.0	37.0
5	35.49049579375991	37.0	35.0	37.0	33.0	37.0
6	35.52180225667283	37.0	35.0	37.0	33.0	37.0
7	35.907691861965795	37.0	35.0	37.0	35.0	37.0
8	35.88847932700158	37.0	35.0	37.0	35.0	37.0
9	37.56579460273814	39.0	37.0	39.0	35.0	39.0
10	36.95566525492923	39.0	37.0	39.0	32.0	39.0
11	36.678086990418095	39.0	35.0	39.0	32.0	39.0
12	36.294334086611144	38.0	35.0	39.0	32.0	39.0
13	36.07962818101845	38.0	35.0	39.0	31.0	39.0
14	37.113755484968685	39.0	35.0	41.0	31.0	41.0
15	37.28389552616192	39.0	35.0	41.0	32.0	41.0
16	37.37756338332359	39.0	35.0	41.0	32.0	41.0
17	37.276001912166755	39.0	35.0	41.0	32.0	41.0
18	37.14517915369501	39.0	35.0	41.0	32.0	41.0
19	37.10851480480623	39.0	35.0	41.0	32.0	41.0
20	36.95365234385289	39.0	35.0	41.0	31.0	41.0
21	36.80843434103994	39.0	35.0	41.0	31.0	41.0
22	36.772507466932154	38.0	35.0	41.0	31.0	41.0
23	36.688185127240736	38.0	35.0	40.0	31.0	41.0
24	36.57301658791489	38.0	35.0	40.0	31.0	41.0
25	36.45931970585291	38.0	35.0	40.0	30.0	41.0
26	36.31829806623577	38.0	35.0	40.0	30.0	41.0
27	36.23095668915965	38.0	35.0	40.0	30.0	41.0
28	36.30790954871811	38.0	35.0	40.0	30.0	41.0
29	36.33189064829301	38.0	35.0	40.0	30.0	41.0
30	36.2752960434478	38.0	35.0	40.0	30.0	41.0
31	36.15469060299099	38.0	35.0	40.0	30.0	41.0
32	36.04232183399443	38.0	35.0	40.0	30.0	41.0
33	35.85083255635097	38.0	35.0	40.0	29.0	41.0
34	35.609835016356136	38.0	34.0	40.0	27.0	41.0
35	35.486751792327105	38.0	34.0	40.0	27.0	41.0
36	35.3540734946296	38.0	34.0	40.0	26.0	41.0
37	35.24459602185033	38.0	34.0	40.0	26.0	41.0
38	35.16870130691066	38.0	34.0	40.0	25.0	41.0
39	35.12720188898898	38.0	34.0	40.0	25.0	41.0
40	34.96045028103057	38.0	34.0	40.0	24.0	41.0
41	34.85815528585049	38.0	34.0	40.0	24.0	41.0
42	34.78296841500867	38.0	34.0	40.0	24.0	41.0
43	34.730793391172426	37.0	33.0	40.0	24.0	41.0
44	34.590852942260994	37.0	33.0	40.0	23.0	41.0
45	34.50317509231604	37.0	33.0	40.0	23.0	41.0
46	34.4335094054373	37.0	33.0	40.0	23.0	41.0
47	34.295425154211244	37.0	33.0	40.0	23.0	41.0
48	34.22982282168387	37.0	33.0	40.0	23.0	41.0
49	34.11533447115157	36.0	33.0	40.0	23.0	41.0
50	34.03570892397162	36.0	33.0	40.0	23.0	41.0
51	33.594644352786865	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	5.0
12	6.0
13	15.0
14	21.0
15	72.0
16	183.0
17	395.0
18	790.0
19	1563.0
20	2538.0
21	4140.0
22	6229.0
23	9052.0
24	12701.0
25	17909.0
26	24386.0
27	28411.0
28	31550.0
29	35230.0
30	40658.0
31	49130.0
32	59814.0
33	75586.0
34	116350.0
35	148123.0
36	121173.0
37	152180.0
38	223041.0
39	357355.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.46858357432956	22.674452293283185	27.780477462243923	15.076486670143332
2	32.249706327006855	24.795021518460576	29.414774253701857	13.540497900830712
3	29.3239068253291	25.098044638294958	31.8057728472321	13.772275689143845
4	26.933105769686627	26.995396050295778	31.100562587904363	14.970935592113236
5	23.649367615375297	31.384622070513124	30.092987668368128	14.873022645743452
6	22.494363585602386	39.80335761732434	27.250944231103524	10.451334565969752
7	86.31325821625921	3.8870847095139514	7.71431543903454	2.0853416351922966
8	86.61344995970227	3.6225814777941077	7.530934433224293	2.233034129279329
9	81.07132698051485	6.462649536181039	9.234303639438044	3.2317198438660535
10	39.662842333159496	33.64761611277043	15.41342046071103	11.27612109335904
11	32.514604634502234	24.386249782708326	25.94949878053277	17.149646802256672
12	32.33879591438971	22.03732675927243	28.676706859042234	16.947170467295628
13	25.946996633954395	25.925925925925924	29.404173053725035	18.722904386394642
14	18.817656726560152	30.530139013996216	30.517101513403606	20.135102746040022
15	18.670820229986777	27.898078351427802	36.042565464056004	17.388535954529413
16	23.49620990639338	24.632447836828437	35.11440077540205	16.756941481376128
17	22.879694158672965	24.49324947191538	29.389818633880644	23.237237735531007
18	23.412453841980224	27.604602896168817	30.97743063786301	18.005512623987947
19	26.514588831471208	27.933042557562544	27.467906677834144	18.084461933132108
20	27.976566738833842	27.67005378298224	27.0918603854886	17.261519092695313
21	26.096598660956506	26.013435210206655	30.306657816969295	17.583308311867548
22	23.66418295695781	23.97056422088423	30.341687869066618	22.023564953091338
23	22.6159152325416	27.16857093190474	30.251676438207515	19.963837397346143
24	23.034563862682194	26.084680541727906	31.449019421925122	19.431736173664778
25	23.104228891101315	27.110824022714226	29.52500039507577	20.259946691108688
26	21.197856582225803	30.441378656426306	27.554823348451567	20.805941412896324
27	20.403622581477794	30.10602516896074	30.510714455032474	18.97963779452899
28	19.01012447520768	28.99843023225188	32.93555787333344	19.055887419207004
29	21.14432381464098	27.677691914642562	31.510256167132855	19.667728103583602
30	21.842488555971702	26.5593640860317	31.58848117068854	20.00966618730806
31	24.24487850102983	26.779355447041407	28.901241591470576	20.074524460458182
32	26.151645885680875	27.24429378888204	27.90295095265939	18.7011093727777
33	24.976953913093862	27.360511912851553	27.963265854390873	19.699268319663712
34	21.318683923576543	27.784362374036675	30.18069449053662	20.716259211850165
35	21.48619605240285	27.74867386231346	29.537576973930268	21.227553111353426
36	24.47494429431565	27.827754863382797	28.12037432112813	19.576926521173426
37	22.040092289701168	29.988687663627218	28.625873775923555	19.345346270748063
38	21.877979529807153	29.89492301290054	26.82064086558469	21.406456591707624
39	21.35358228374869	27.945948366229977	28.47758866817322	22.22288068184811
40	23.804698241122647	26.397317172100276	28.79509789977718	21.0028866869999
41	20.550261540163405	26.254958200982948	29.672100275499506	23.52267998335414
42	21.84696608142775	28.334637083392593	27.460729467911943	22.35766736726771
43	22.036734145609127	27.3083619104811	27.926457961303647	22.72844598260613
44	22.01322713696487	27.925206888014454	28.432154953986842	21.629411021033835
45	21.24434383181361	29.190963826861992	27.03220394338301	22.532488397941393
46	21.88930503537245	29.26767437327813	27.89965865452994	20.943361936819482
47	22.134778783904085	28.207093453857784	28.532306663084643	21.125821099153484
48	22.0309397009013	27.222498775265098	29.523354246011053	21.223207277822553
49	21.563499212482288	26.448216101181536	29.48391251442027	22.504372171915907
50	19.830960244841627	28.62824423057676	29.126566475449987	22.414229049131624
51	19.683465288642363	29.334705563193687	28.290059366719873	22.691769781444084
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1957.0
1	2613.0
2	3269.0
3	10960.0
4	18651.0
5	13222.0
6	7793.0
7	7906.5
8	8020.0
9	8354.0
10	8688.0
11	8600.5
12	8513.0
13	8242.5
14	7972.0
15	7609.0
16	7246.0
17	7358.0
18	7470.0
19	7245.5
20	7021.0
21	7419.5
22	7818.0
23	8761.0
24	9704.0
25	10756.5
26	14814.0
27	17819.0
28	20472.0
29	23125.0
30	25825.5
31	28526.0
32	32493.0
33	36460.0
34	43068.5
35	49677.0
36	52006.5
37	54336.0
38	60811.0
39	67286.0
40	75369.5
41	83453.0
42	94284.0
43	105115.0
44	108832.0
45	112549.0
46	112259.0
47	111969.0
48	112134.0
49	112299.0
50	109618.0
51	106937.0
52	100672.5
53	94408.0
54	86521.0
55	78634.0
56	74237.0
57	69840.0
58	70727.0
59	71614.0
60	65470.5
61	59327.0
62	50321.0
63	41315.0
64	35182.0
65	29049.0
66	23929.5
67	18810.0
68	15044.5
69	11279.0
70	9228.5
71	7178.0
72	6335.0
73	5492.0
74	4231.5
75	2313.5
76	1656.0
77	1284.5
78	913.0
79	645.5
80	378.0
81	285.0
82	192.0
83	138.0
84	84.0
85	65.0
86	46.0
87	28.0
88	10.0
89	9.5
90	9.0
91	6.5
92	4.0
93	3.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1518696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.25704532237507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.07633620628172	17.447003986314247
2	6.630726207695495	2.818992950344866
3	2.0283225310519333	1.2934843191289636
4	1.0678713766173429	0.9079916100488765
5	0.6836550671494822	0.7266243373633957
6	0.471839854272225	0.6017952700300521
7	0.35259058516999775	0.5246523834440973
8	0.28873789663246524	0.49101716440028487
9	0.2328897329672805	0.4455492847921178
>10	3.449976959039695	18.606891585325755
>50	1.709365514448282	26.563902085701237
>100	1.0036411286391855	28.158158653886968
>500	0.0024904246368208332	0.3336484847476597
>1k	0.0012452123184104166	0.3603165409462529
>5k	0.0	0.0
>10k+	3.1130307960260416E-4	0.719971343525295
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10880	0.7164040729678619	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028577147763607726	0.0
2	6.58459625889579E-5	0.0	0.0	0.11299167180265175	0.0
3	6.58459625889579E-5	0.0	0.0	0.15243340339343753	0.0
4	6.58459625889579E-5	0.0	0.0	0.23414824296633427	0.0
5	6.58459625889579E-5	0.0	0.0	0.40119945005452046	0.0
6	6.58459625889579E-5	0.0	0.0	0.5774690919051607	0.0
7	6.58459625889579E-5	0.0	0.0	0.6798595637309903	0.0
8	6.58459625889579E-5	0.0	0.0	0.9076865942887846	0.0
9	6.58459625889579E-5	0.0	0.0	1.002307242529117	0.0
10	6.58459625889579E-5	0.0	0.0	1.2207841463992795	0.0
11	6.58459625889579E-5	0.0	0.0	1.5235438823833078	0.0
12	1.316919251779158E-4	0.0	0.0	1.7727708507825135	0.0
13	1.316919251779158E-4	0.0	0.0	1.8733834816184411	0.0
14	1.316919251779158E-4	0.0	0.0	1.9186855038796442	0.0
15	1.316919251779158E-4	0.0	0.0	1.986243461495915	0.0
16	1.316919251779158E-4	0.0	0.0	2.1196473817011436	0.0
17	1.316919251779158E-4	0.0	0.0	2.2721466310571703	0.0
18	1.316919251779158E-4	0.0	0.0	2.4541448716530496	0.0
19	1.316919251779158E-4	0.0	0.0	2.558444876393959	0.0
20	1.316919251779158E-4	0.0	0.0	2.677033455016672	0.0
21	1.316919251779158E-4	0.0	0.0	2.8222238025253246	0.0
22	1.9753788776687368E-4	0.0	0.0	2.977225198459731	0.0
23	1.9753788776687368E-4	0.0	0.0	3.1364407359998316	0.0
24	1.9753788776687368E-4	0.0	0.0	3.2547000848096	0.0
25	1.9753788776687368E-4	0.0	0.0	3.363740998856914	0.0
26	1.9753788776687368E-4	0.0	0.0	3.4694896147747807	0.0
27	1.9753788776687368E-4	0.0	0.0	3.584259127567334	0.0
28	1.9753788776687368E-4	0.0	0.0	3.695012036641961	0.0
29	1.9753788776687368E-4	0.0	0.0	3.823740893503374	0.0
30	1.9753788776687368E-4	0.0	0.0	3.9793349031010816	0.0
31	1.9753788776687368E-4	0.0	0.0	4.115438507772457	0.0
32	1.9753788776687368E-4	0.0	0.0	4.239623993215232	0.0
33	1.9753788776687368E-4	0.0	0.0	4.374805754410363	0.0
34	1.9753788776687368E-4	0.0	0.0	4.512621354109052	0.0
35	1.9753788776687368E-4	0.0	0.0	4.656692320253692	0.0
36	2.633838503558316E-4	0.0	0.0	4.79575899324157	0.0
37	2.633838503558316E-4	0.0	0.0	4.941410262488345	0.0
38	2.633838503558316E-4	0.0	0.0	5.112017151556335	0.0
39	2.633838503558316E-4	0.0	0.0	5.378693300041615	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	20	7.034004E-4	45.0	1
CGTTATA	20	7.034004E-4	45.0	38
CGCTATT	20	7.034004E-4	45.0	7
TATACGA	20	7.034004E-4	45.0	13
TAGTACG	25	3.8914E-5	45.0	1
CTATGCG	65	0.0	44.999996	1
TCCGATT	30	2.1659653E-6	44.999996	13
ACTCGAT	60	0.0	44.999996	20
GCGATAA	115	0.0	43.04348	9
ACGGGTA	90	0.0	42.5	5
CGTTTTT	6195	0.0	42.16707	1
TACGAAA	215	0.0	41.860466	20
ATACGAG	70	0.0	41.785713	1
CGACGGT	375	0.0	41.4	28
CGGTCTA	370	0.0	41.351353	31
TCGAATA	50	1.0822987E-9	40.5	38
CACGACG	385	0.0	40.324673	26
TCTAGCG	90	0.0	40.0	1
GGGCGAT	1635	0.0	39.770645	7
CGTAAGG	165	0.0	39.545456	2
>>END_MODULE