FastQCFastQC Report
Thu 26 May 2016
SRR1547355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547355_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences638005
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103131.616445012186425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8790.13777321494345657No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7190.1126950415749093No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6590.10329072656170406No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGA207.030924E-445.00000421
CAAACGT207.030924E-445.00000435
TAGACGT253.8888465E-545.00000420
GACGTAG502.1827873E-1145.0000041
TAGCGCG253.8888465E-545.0000041
GTTAGCG253.8888465E-545.0000041
TTCCGCG207.030924E-445.00000417
ATATGCG207.030924E-445.0000041
TCTAGCG207.030924E-445.0000041
TTATACG207.030924E-445.0000041
GTTAACG207.030924E-445.0000041
CGGGCTA207.030924E-445.0000046
GTCGTTA207.030924E-445.00000436
GCGATAT406.8066583E-945.0000049
CGGTCTA850.045.00000431
CCGGTAC207.030924E-445.00000435
ACGTAAC207.030924E-445.00000438
AGGATAC207.030924E-445.0000046
GTGACGC253.8888465E-545.00000420
TATAACG253.8888465E-545.0000041