##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547355_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	638005
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262626468444605	31.0	31.0	33.0	30.0	34.0
2	31.667734578882612	31.0	31.0	34.0	30.0	34.0
3	31.69525160461125	31.0	31.0	34.0	30.0	34.0
4	35.524014702079135	37.0	35.0	37.0	33.0	37.0
5	35.4161660175077	37.0	35.0	37.0	33.0	37.0
6	35.4688944444009	37.0	35.0	37.0	33.0	37.0
7	35.884482096535294	37.0	35.0	37.0	35.0	37.0
8	35.85843214394872	37.0	35.0	37.0	35.0	37.0
9	37.552332661969736	39.0	37.0	39.0	35.0	39.0
10	36.86976277615379	39.0	37.0	39.0	32.0	39.0
11	36.64521594658349	39.0	35.0	39.0	32.0	39.0
12	36.3100743724579	38.0	35.0	39.0	32.0	39.0
13	36.13132812438774	38.0	35.0	39.0	31.0	39.0
14	37.27595238281832	39.0	35.0	41.0	32.0	41.0
15	37.41449675159286	39.0	35.0	41.0	32.0	41.0
16	37.479676491563545	39.0	35.0	41.0	32.0	41.0
17	37.40527582072241	39.0	35.0	41.0	32.0	41.0
18	37.24783348092883	39.0	36.0	41.0	32.0	41.0
19	37.12288148212004	38.0	35.0	41.0	32.0	41.0
20	36.90763238532613	38.0	35.0	41.0	32.0	41.0
21	36.791496931842225	38.0	35.0	41.0	31.0	41.0
22	36.671897555661786	38.0	35.0	40.0	31.0	41.0
23	36.59022578192961	38.0	35.0	40.0	31.0	41.0
24	36.44436642346063	38.0	35.0	40.0	31.0	41.0
25	36.3410568882689	38.0	35.0	40.0	31.0	41.0
26	36.17523530379856	38.0	35.0	40.0	30.0	41.0
27	36.09816850965118	38.0	35.0	40.0	30.0	41.0
28	36.11874828567174	38.0	35.0	40.0	30.0	41.0
29	36.11811035963668	38.0	35.0	40.0	30.0	41.0
30	36.04941183846522	38.0	35.0	40.0	30.0	41.0
31	35.914293775127156	38.0	35.0	40.0	30.0	41.0
32	35.71211981097327	38.0	35.0	40.0	29.0	41.0
33	35.4315467747118	38.0	34.0	40.0	27.0	41.0
34	35.16850024686327	38.0	34.0	40.0	25.0	41.0
35	34.962702486657626	38.0	34.0	40.0	24.0	41.0
36	34.757900016457555	38.0	34.0	40.0	23.0	41.0
37	34.644209684877076	38.0	34.0	40.0	23.0	41.0
38	34.589297889514974	38.0	34.0	40.0	23.0	41.0
39	34.54749570928128	38.0	34.0	40.0	23.0	41.0
40	34.40545920486517	37.0	33.0	40.0	23.0	41.0
41	34.351061512057115	37.0	33.0	40.0	23.0	41.0
42	34.25638984020502	37.0	33.0	40.0	22.0	41.0
43	34.21150147726115	37.0	33.0	40.0	22.0	41.0
44	34.077369299613636	37.0	33.0	40.0	22.0	41.0
45	33.99760189967163	37.0	33.0	40.0	22.0	41.0
46	33.8958362395279	37.0	33.0	40.0	21.0	41.0
47	33.75393766506532	36.0	33.0	40.0	20.0	41.0
48	33.68686765777698	36.0	33.0	40.0	20.0	41.0
49	33.5802478037006	36.0	33.0	40.0	20.0	41.0
50	33.51146464369401	36.0	33.0	40.0	20.0	41.0
51	33.0705307952132	35.0	32.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	3.0
14	9.0
15	29.0
16	72.0
17	157.0
18	338.0
19	682.0
20	1212.0
21	1928.0
22	2853.0
23	4334.0
24	6235.0
25	9325.0
26	12470.0
27	14169.0
28	14628.0
29	15773.0
30	17422.0
31	20727.0
32	25182.0
33	31797.0
34	47413.0
35	64872.0
36	55374.0
37	62406.0
38	88629.0
39	139901.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.302105782870036	23.616742815495176	27.409189583153736	17.671961818481048
2	34.447849154787185	24.855761318484966	27.717494377003316	12.978895149724531
3	27.469690676405357	25.365788669367795	34.33781866913269	12.82670198509416
4	25.00074450827188	26.717658952516043	34.03092452253509	14.250672016676985
5	21.70641295914609	31.6484980525231	32.84253258203306	13.802556406297755
6	20.18981042468319	39.640598427912	30.3607338500482	9.808857297356603
7	83.7684657643749	3.767055117122906	10.758536375106779	1.7059427433954282
8	83.77332466046504	3.8095312732658835	10.507597902837752	1.909546163431321
9	78.69718889350396	6.713427010760103	11.938777909264035	2.650606186471893
10	36.064921121307826	32.648960431344584	19.369754155531695	11.916364291815896
11	30.798661452496457	25.445411869812933	27.120006896497678	16.635919781192936
12	29.20980243101543	22.90624681624752	30.842234778724304	17.041715974012742
13	21.522558600637925	29.789421712995978	31.53862430545215	17.149395380913944
14	17.370396783724264	32.84394322928504	31.181887289284564	18.60377269770613
15	17.19970846623459	29.232529525630678	37.241087452292696	16.32667455584204
16	18.83511884703098	27.154175907712325	37.11428593819798	16.89641930705872
17	18.820855635927618	28.077679642009073	30.237537323375207	22.863927398688098
18	20.176174167914045	29.769045697134032	31.461665660927423	18.593114474024496
19	24.07974859131198	30.789100398899695	27.98630104779743	17.144849961990893
20	25.308735824954347	28.28896325263909	29.77468828614196	16.627612636264605
21	22.7013894875432	29.635504423946518	30.27029568733787	17.392810401172405
22	22.06393366823144	26.755589689735977	29.940204230374366	21.240272411658214
23	20.21394816655042	30.398194371517462	30.013087671726712	19.374769790205406
24	19.32006802454526	28.24930839100007	34.331392387206996	18.09923119724767
25	19.728685511869028	30.806184904507017	31.106809507762478	18.358320075861474
26	18.255186087883324	35.104740558459575	28.128619681663935	18.511453671993166
27	19.287623137749705	32.95945956536391	30.272019811756962	17.480897485129425
28	17.700174763520664	31.191448342881323	34.14314934835934	16.965227545238672
29	18.509416070406974	28.661844342912673	32.99989811992069	19.828841466759663
30	18.248759805957633	31.680786200735106	31.641601554846748	18.428852438460513
31	22.825839922884615	30.36230123588373	28.218901105790707	18.592957735440947
32	22.643866427379095	31.247404017209895	28.37015383891976	17.73857571649125
33	21.688858237788107	30.659791067468124	27.770315279660817	19.881035415082955
34	18.881513467762794	30.875620097021184	28.96466328633788	21.278203148878145
35	20.166926591484394	29.10572801153596	29.98142647784892	20.74591891913073
36	22.727094615245964	30.968722815651915	27.520160500309558	18.784022068792563
37	19.469283156088117	32.711969341933056	29.925941019271008	17.892806482707815
38	19.805957633560865	32.74316032006019	27.784421752180627	19.66646029419832
39	20.030407285209364	31.748497268830185	28.15620567236934	20.064889773591116
40	20.920368962625684	31.14082178039357	28.616076676515075	19.322732580465672
41	18.30753677479017	30.159168031598497	29.643027875957085	21.89026731765425
42	19.986677220397958	31.241447951034868	27.522354840479306	21.24951998808787
43	20.115829813245977	30.717470866215784	27.804014075124805	21.362685245413438
44	19.451728434730136	32.57513655849092	28.016551594423238	19.9565834123557
45	19.227748998832297	34.304119873668704	25.55246432237992	20.91566680511908
46	20.270687533796757	34.41368014357254	25.3085790863708	20.007053236259903
47	20.531657275413203	30.857751898496094	28.36482472707894	20.245766099011764
48	19.973667917963027	29.85822995117593	29.71606805589298	20.452034074968065
49	18.895776678866152	30.16089215601759	29.91042389949922	21.03290726561704
50	18.019764735386087	31.660253446289605	29.741929922179295	20.578051896145013
51	17.75095806459197	32.05570489259488	28.153384377865375	22.03995266494777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1041.0
1	1078.0
2	1115.0
3	7822.0
4	14529.0
5	9908.0
6	5287.0
7	5449.5
8	5612.0
9	6305.5
10	6999.0
11	7421.5
12	7844.0
13	7786.5
14	7729.0
15	7293.0
16	6857.0
17	6191.5
18	5526.0
19	5144.0
20	4762.0
21	4701.0
22	4640.0
23	4950.5
24	5261.0
25	5456.0
26	6945.0
27	8239.0
28	10057.0
29	11875.0
30	13304.5
31	14734.0
32	17139.0
33	19544.0
34	20958.0
35	22372.0
36	23066.0
37	23760.0
38	26379.0
39	28998.0
40	33054.0
41	37110.0
42	42205.5
43	47301.0
44	47389.5
45	47478.0
46	48260.0
47	49042.0
48	47826.0
49	46610.0
50	43412.0
51	40214.0
52	36278.5
53	32343.0
54	29928.0
55	27513.0
56	25487.0
57	23461.0
58	22310.0
59	21159.0
60	19791.0
61	18423.0
62	15466.5
63	12510.0
64	10472.0
65	8434.0
66	6861.0
67	5288.0
68	4364.5
69	3441.0
70	2719.0
71	1997.0
72	1794.0
73	1591.0
74	1181.0
75	651.0
76	531.0
77	366.5
78	202.0
79	153.5
80	105.0
81	70.5
82	36.0
83	34.5
84	33.0
85	23.5
86	14.0
87	11.5
88	9.0
89	11.0
90	13.0
91	7.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	638005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.077524269494205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.64716543757496	24.130816683436677
2	7.605707737949167	4.727331336255903
3	3.1385338603673847	2.9261358664859025
4	1.85646639127857	2.3077751732184035
5	1.2367986030040492	1.9218319300667437
6	0.8583305459677136	1.6004873024135902
7	0.7139659156314162	1.5531805149438895
8	0.5323507000110488	1.323531343958048
9	0.4606770317013384	1.2885031469787205
>10	5.08810323268086	37.5030586947958
>50	0.8076515096039794	15.852743392644802
>100	0.04968603135038903	2.4386048332993253
>500	0.004056002559215431	0.8010510417066465
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	5.070003199019289E-4	1.6249487397955638
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10313	1.616445012186425	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	879	0.13777321494345657	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	719	0.1126950415749093	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	659	0.10329072656170406	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05235068690684242	0.0
2	0.0	0.0	0.0	0.17366635057718982	0.0
3	0.0	0.0	0.0	0.2655151605394942	0.0
4	0.0	0.0	0.0	0.4341658764429746	0.0
5	0.0	0.0	0.0	0.8192725762337286	0.0
6	0.0	0.0	0.0	1.4294558820071943	0.0
7	0.0	0.0	0.0	1.7982617691083926	0.0
8	0.0	0.0	0.0	2.3167530035031074	0.0
9	0.0	0.0	0.0	2.534306157475255	0.0
10	0.0	0.0	0.0	2.8554635151762136	0.0
11	0.0	0.0	0.0	3.2037366478319136	0.0
12	0.0	0.0	0.0	3.5206620637769297	0.0
13	0.0	0.0	0.0	3.665331776396737	0.0
14	0.0	0.0	0.0	3.742760636672126	0.0
15	0.0	0.0	0.0	3.8128227835205055	0.0
16	0.0	0.0	0.0	3.9634485623153424	0.0
17	0.0	0.0	0.0	4.145108580653757	0.0
18	0.0	0.0	0.0	4.356548929867321	0.0
19	0.0	0.0	0.0	4.483977398296251	0.0
20	0.0	0.0	0.0	4.615481069897571	0.0
21	0.0	0.0	0.0	4.79917868982218	0.0
22	0.0	0.0	0.0	4.993691272011975	0.0
23	0.0	0.0	0.0	5.234441736350028	0.0
24	0.0	0.0	0.0	5.3938448758238575	0.0
25	0.0	0.0	0.0	5.545097608952908	0.0
26	0.0	0.0	0.0	5.7005822838379006	0.0
27	0.0	0.0	0.0	5.84854350671233	0.0
28	0.0	0.0	0.0	6.0071629532683914	0.0
29	0.0	0.0	0.0	6.172835636084357	0.0
30	0.0	0.0	0.0	6.371109944279434	0.0
31	0.0	0.0	0.0	6.55653168862313	0.0
32	0.0	0.0	0.0	6.734900196706922	0.0
33	0.0	0.0	0.0	6.9102906717031995	0.0
34	0.0	0.0	0.0	7.0866215782007975	0.0
35	0.0	0.0	0.0	7.289598043902477	0.0
36	0.0	0.0	0.0	7.484894319010039	0.0
37	0.0	0.0	0.0	7.687400568961058	0.0
38	0.0	0.0	0.0	7.8906905118298445	0.0
39	0.0	0.0	0.0	8.093823716115077	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGA	20	7.030924E-4	45.000004	21
CAAACGT	20	7.030924E-4	45.000004	35
TAGACGT	25	3.8888465E-5	45.000004	20
GACGTAG	50	2.1827873E-11	45.000004	1
TAGCGCG	25	3.8888465E-5	45.000004	1
GTTAGCG	25	3.8888465E-5	45.000004	1
TTCCGCG	20	7.030924E-4	45.000004	17
ATATGCG	20	7.030924E-4	45.000004	1
TCTAGCG	20	7.030924E-4	45.000004	1
TTATACG	20	7.030924E-4	45.000004	1
GTTAACG	20	7.030924E-4	45.000004	1
CGGGCTA	20	7.030924E-4	45.000004	6
GTCGTTA	20	7.030924E-4	45.000004	36
GCGATAT	40	6.8066583E-9	45.000004	9
CGGTCTA	85	0.0	45.000004	31
CCGGTAC	20	7.030924E-4	45.000004	35
ACGTAAC	20	7.030924E-4	45.000004	38
AGGATAC	20	7.030924E-4	45.000004	6
GTGACGC	25	3.8888465E-5	45.000004	20
TATAACG	25	3.8888465E-5	45.000004	1
>>END_MODULE