FastQCFastQC Report
Thu 26 May 2016
SRR1547354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547354_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4842230
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1065062.1995237731375834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT511800.043.8218081
CGTTAGG5950.038.949582
GTTTTTT600900.038.0204662
TACGCGG2150.037.674422
GGGCGAT55350.037.6016247
CGGTCTA5750.037.56521631
GCGATCG2400.037.4999969
TAGGGAT67700.037.1898085
AGGGCGA27550.037.159716
TAGGGAC50150.037.0588235
AGGGATT72650.036.8547826
ACGGGTA5950.036.680675
ACGGGAC17750.036.6338045
ATAGCGG5850.036.538462
GTAGGGA72000.036.43754
TAAGGGA60650.036.3190464
CGACGGT5900.036.22881328
GTAGGGT26500.036.04
TAGGGTG22800.035.9210555
AAGGGAT67450.035.8598985
Produced by FastQC (version 0.11.5)