##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547354_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4842230
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.326628846626452	31.0	31.0	33.0	30.0	34.0
2	31.735465271166383	31.0	31.0	34.0	30.0	34.0
3	31.717204056808537	31.0	31.0	34.0	30.0	34.0
4	35.570249038149775	37.0	35.0	37.0	33.0	37.0
5	35.49118567271691	37.0	35.0	37.0	33.0	37.0
6	35.54180924904435	37.0	35.0	37.0	33.0	37.0
7	35.93695859139281	37.0	35.0	37.0	35.0	37.0
8	35.92160863899484	37.0	35.0	37.0	35.0	37.0
9	37.624699776755754	39.0	37.0	39.0	35.0	39.0
10	36.941845389417686	39.0	37.0	39.0	32.0	39.0
11	36.74178591268899	39.0	37.0	39.0	32.0	39.0
12	36.508514258926155	39.0	35.0	39.0	32.0	39.0
13	36.42283741168842	39.0	35.0	39.0	32.0	39.0
14	37.51985882537591	40.0	36.0	41.0	32.0	41.0
15	37.608700123703336	40.0	36.0	41.0	32.0	41.0
16	37.635057814271526	40.0	36.0	41.0	32.0	41.0
17	37.53692141843737	40.0	36.0	41.0	32.0	41.0
18	37.31523802049882	39.0	36.0	41.0	32.0	41.0
19	37.16036970569345	39.0	36.0	41.0	32.0	41.0
20	36.90075853480731	38.0	35.0	41.0	31.0	41.0
21	36.790838518616425	38.0	35.0	41.0	31.0	41.0
22	36.734143153051384	38.0	35.0	40.0	31.0	41.0
23	36.64486672462894	38.0	35.0	40.0	31.0	41.0
24	36.50389902999238	38.0	35.0	40.0	31.0	41.0
25	36.42621891153456	38.0	35.0	40.0	31.0	41.0
26	36.30961230672644	38.0	35.0	40.0	30.0	41.0
27	36.220937460632804	38.0	35.0	40.0	30.0	41.0
28	36.24223260770348	38.0	35.0	40.0	30.0	41.0
29	36.20126036970569	38.0	35.0	40.0	30.0	41.0
30	36.09088395222862	38.0	35.0	40.0	30.0	41.0
31	35.9190689000729	38.0	35.0	40.0	29.0	41.0
32	35.67860675763026	38.0	35.0	40.0	28.0	41.0
33	35.30499274094787	38.0	34.0	40.0	25.0	41.0
34	34.8948511326393	38.0	34.0	40.0	23.0	41.0
35	34.62534039068776	38.0	34.0	40.0	22.0	41.0
36	34.44861210640552	38.0	33.0	40.0	21.0	41.0
37	34.357219091203845	38.0	33.0	40.0	21.0	41.0
38	34.245697127150095	38.0	33.0	40.0	20.0	41.0
39	34.18888631890678	38.0	33.0	40.0	20.0	41.0
40	34.03058879896246	38.0	33.0	40.0	18.0	41.0
41	33.92296999522947	38.0	33.0	40.0	18.0	41.0
42	33.847910776646295	37.0	33.0	40.0	18.0	41.0
43	33.79673435586496	37.0	33.0	40.0	18.0	41.0
44	33.66960078311026	37.0	33.0	40.0	18.0	41.0
45	33.5765698861888	37.0	33.0	40.0	18.0	41.0
46	33.52138580777865	37.0	33.0	40.0	18.0	41.0
47	33.391752147254465	37.0	32.0	40.0	18.0	41.0
48	33.33800129279278	36.0	32.0	40.0	18.0	41.0
49	33.213111107898634	36.0	32.0	40.0	18.0	41.0
50	33.14857266176947	36.0	32.0	40.0	18.0	41.0
51	32.74125764368896	35.0	31.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	1.0
11	4.0
12	21.0
13	26.0
14	83.0
15	191.0
16	562.0
17	1366.0
18	3026.0
19	5628.0
20	9734.0
21	15189.0
22	22865.0
23	33706.0
24	50028.0
25	77043.0
26	109681.0
27	130353.0
28	131398.0
29	130081.0
30	138101.0
31	156079.0
32	183862.0
33	228049.0
34	326701.0
35	407738.0
36	394608.0
37	478815.0
38	699775.0
39	1107194.0
40	318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.981475477207812	21.79033214035682	26.827721938032685	21.400470444402682
2	36.83226529925261	23.55584100713927	27.272847427734742	12.33904626587337
3	27.013029120880255	23.5732916445522	37.03023193859028	12.383447295977266
4	24.358446418282487	25.579309533004423	36.619821858936895	13.442422189776199
5	21.412407093425966	29.45269018613325	35.54715492655244	13.587747793888353
6	20.35603430650754	37.861625738554345	32.14436323759921	9.637976717338912
7	82.0219403043639	3.035481585963492	13.466543307525663	1.4760348021469447
8	82.52201981318524	2.568857737034383	13.321341613264961	1.5877808365154071
9	77.66279173025652	5.03439530959909	14.894769558653762	2.4080434014906356
10	32.73983268039726	32.595890736292986	24.472071752064647	10.192204831245109
11	27.188712638598332	22.969891971261173	32.49779543722624	17.343599952914257
12	26.749307653705007	21.108621440947662	34.39177816832327	17.750292737024058
13	23.934509513178845	21.785148578237713	35.0346637809439	19.245678127639536
14	18.886793894548585	25.40519554007141	36.39296357256884	19.315046992811162
15	19.284998853833873	25.677693955057894	37.873314567874715	17.163992623233508
16	23.59617366378714	24.851132639300488	35.74902472621086	15.803668970701516
17	23.220127916269984	24.7440332243615	33.50154784056107	18.53429101880745
18	22.57575538543192	26.073668537017035	34.26780636194481	17.08276971560624
19	23.558133339391148	26.929204106372477	32.402240290114264	17.110422264122107
20	24.47260869475428	27.036055701608557	32.38567767330341	16.10565793033375
21	24.597076140538555	25.097919760110525	34.451729884784484	15.85327421456643
22	23.210359689647124	22.4486032261995	35.82427930932649	18.516757774826885
23	21.415236368367466	24.355988872895338	35.58009429539696	18.648680463340238
24	20.748539412626002	25.259147128492447	35.93693401593894	18.055379442942613
25	22.166460494441612	26.483500370696973	33.04578675527598	18.30425237958544
26	20.656556999564252	27.55019897856979	32.30168744566036	19.49155657620559
27	19.225604731704195	26.95239590023605	34.88485677053754	18.937142597522218
28	18.395615243389926	27.252980548218485	35.34202216747243	19.009382040919164
29	20.11007325137385	26.577899025862052	34.50102535402077	18.811002368743328
30	20.892192233743543	25.80670476206211	35.03807130185885	18.263031702335493
31	22.341441856334786	25.477331725258818	33.24532291939871	18.93590349900769
32	22.856576412107646	26.432242995479356	32.384769001059425	18.32641159135357
33	22.222736218643064	26.63549645514567	32.026607575435285	19.115159750775984
34	20.23228966819007	27.599783570792795	33.131738888900365	19.036187872116773
35	20.244350227064803	28.26363059995085	31.758631870026825	19.73338730295752
36	21.61590837279518	29.438048172019915	30.032010044958625	18.91403341022628
37	20.99363309879952	29.69241444541048	29.829087011562855	19.484865444227143
38	21.078139617490287	29.7079651317678	29.34837874285195	19.86551650788996
39	21.026159434805862	28.478882663566168	29.594959347242906	20.899998554385064
40	21.8384504660043	27.379182731923102	30.643050825755903	20.139315976316695
41	19.948019817315576	27.618411351794524	31.064881263384848	21.368687567505056
42	20.50485003810228	28.327568083300463	29.805523488144924	21.362058390452333
43	20.1771497842936	27.695710447459128	30.52170177789985	21.605437990347422
44	20.76338794315842	27.92688492698612	30.48184410901589	20.82788302083957
45	20.902352841562667	27.8819676058345	29.803499627237862	21.412179925364967
46	20.924429446763167	28.564421764352378	29.76843313927674	20.74271564960772
47	20.75907174999948	28.191329201628175	30.58758051558889	20.46201853278345
48	20.3387901855137	27.513996650303685	31.794916804860566	20.352296359322047
49	21.17774248641638	27.103958300204656	31.16741666546199	20.55088254791697
50	19.982631969154706	27.52393009006181	31.662870206495768	20.830567734287715
51	19.334314974712065	27.7471123841701	31.91133011030042	21.007242530817415
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5021.0
1	6687.0
2	8353.0
3	87087.0
4	165821.0
5	111831.5
6	57842.0
7	56119.0
8	54396.0
9	55700.0
10	57004.0
11	55999.0
12	54994.0
13	52423.0
14	49852.0
15	46718.0
16	43584.0
17	40934.0
18	38284.0
19	36210.5
20	34137.0
21	33910.5
22	33684.0
23	34508.0
24	35332.0
25	39929.0
26	52506.5
27	60487.0
28	68361.0
29	76235.0
30	85491.5
31	94748.0
32	107673.5
33	120599.0
34	133340.5
35	146082.0
36	163665.5
37	181249.0
38	188823.0
39	196397.0
40	219612.0
41	242827.0
42	260638.5
43	278450.0
44	299143.5
45	319837.0
46	326517.0
47	333197.0
48	332951.0
49	332705.0
50	313527.5
51	294350.0
52	280192.5
53	266035.0
54	254404.5
55	242774.0
56	237242.5
57	231711.0
58	219232.0
59	206753.0
60	190839.0
61	174925.0
62	152556.5
63	130188.0
64	109904.5
65	89621.0
66	72202.5
67	54784.0
68	45289.5
69	35795.0
70	28997.0
71	22199.0
72	17537.0
73	12875.0
74	10164.0
75	5515.5
76	3578.0
77	2773.5
78	1969.0
79	1430.0
80	891.0
81	611.0
82	331.0
83	253.5
84	176.0
85	124.0
86	72.0
87	60.0
88	48.0
89	38.0
90	28.0
91	22.5
92	17.0
93	11.5
94	6.0
95	6.5
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4842230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.14867359766896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.43055568497462	18.035787987433093
2	6.065307173412996	2.6867701770704917
3	2.1828218213173107	1.4503982412667915
4	1.1317749021616685	1.0026925157605007
5	0.8102778431280655	0.8973289735433367
6	0.6358686211305128	0.8450187924251732
7	0.5173377893345076	0.8020842084996742
8	0.3855184076067654	0.6830977100780277
9	0.32418446365581616	0.6462230283853242
>10	4.119865812793923	22.56544055916864
>50	1.7339975449198723	27.62839603826126
>100	0.6555783427826827	18.89184520601755
>500	0.004165891539784087	0.6367817277976336
>1k	0.002651021888328031	0.994680487693114
>5k	0.0	0.0
>10k+	9.467935315457254E-5	2.2334543465992622
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106506	2.1995237731375834	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.130328381757992E-5	0.0	0.0	0.025215654770632537	0.0
2	4.130328381757992E-5	0.0	0.0	0.07639042342061406	0.0
3	4.130328381757992E-5	0.0	0.0	0.12795757326686258	0.0
4	4.130328381757992E-5	0.0	0.0	0.2093456940294038	0.0
5	4.130328381757992E-5	0.0	0.0	0.4003940333276197	0.0
6	4.130328381757992E-5	0.0	0.0	0.7823874537145076	0.0
7	4.130328381757992E-5	0.0	0.0	1.0440024534150587	0.0
8	4.130328381757992E-5	0.0	0.0	1.4191395286882285	0.0
9	4.130328381757992E-5	0.0	0.0	1.6617550178326927	0.0
10	4.130328381757992E-5	0.0	0.0	1.9491845699192314	0.0
11	4.130328381757992E-5	0.0	0.0	2.174163556873589	0.0
12	4.130328381757992E-5	0.0	0.0	2.3355974416746004	0.0
13	4.130328381757992E-5	0.0	0.0	2.428467875338429	0.0
14	4.130328381757992E-5	0.0	0.0	2.4969074166241585	0.0
15	4.130328381757992E-5	0.0	0.0	2.5484539148284986	0.0
16	4.130328381757992E-5	0.0	0.0	2.620073808968182	0.0
17	4.130328381757992E-5	0.0	0.0	2.7017923560012638	0.0
18	4.130328381757992E-5	0.0	0.0	2.8038940735983213	0.0
19	4.130328381757992E-5	0.0	0.0	2.8766498080429885	0.0
20	4.130328381757992E-5	0.0	0.0	2.9571086049196342	0.0
21	4.130328381757992E-5	0.0	0.0	3.052973526660237	0.0
22	4.130328381757992E-5	0.0	0.0	3.164802167596335	0.0
23	4.130328381757992E-5	0.0	0.0	3.2826197846859815	0.0
24	4.130328381757992E-5	0.0	0.0	3.3796825016572942	0.0
25	4.130328381757992E-5	0.0	0.0	3.4696410538119835	0.0
26	4.130328381757992E-5	0.0	0.0	3.5565431629641715	0.0
27	4.130328381757992E-5	0.0	0.0	3.6532961053068522	0.0
28	4.130328381757992E-5	0.0	0.0	3.756306495147897	0.0
29	4.130328381757992E-5	0.0	0.0	3.8754045140358886	0.0
30	4.130328381757992E-5	0.0	0.0	4.014844400204038	0.0
31	4.130328381757992E-5	0.0	0.0	4.135222820890375	0.0
32	4.130328381757992E-5	0.0	0.0	4.255188208738535	0.0
33	4.130328381757992E-5	0.0	0.0	4.372097153584196	0.0
34	6.195492572636988E-5	0.0	0.0	4.502099239400028	0.0
35	6.195492572636988E-5	0.0	0.0	4.636500124942433	0.0
36	6.195492572636988E-5	0.0	0.0	4.764932685973198	0.0
37	6.195492572636988E-5	0.0	0.0	4.9020802398894725	0.0
38	6.195492572636988E-5	0.0	0.0	5.087531984230406	0.0
39	6.195492572636988E-5	0.0	0.0	5.366494363134341	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	51180	0.0	43.821808	1
CGTTAGG	595	0.0	38.94958	2
GTTTTTT	60090	0.0	38.020466	2
TACGCGG	215	0.0	37.67442	2
GGGCGAT	5535	0.0	37.601624	7
CGGTCTA	575	0.0	37.565216	31
GCGATCG	240	0.0	37.499996	9
TAGGGAT	6770	0.0	37.189808	5
AGGGCGA	2755	0.0	37.15971	6
TAGGGAC	5015	0.0	37.058823	5
AGGGATT	7265	0.0	36.854782	6
ACGGGTA	595	0.0	36.68067	5
ACGGGAC	1775	0.0	36.633804	5
ATAGCGG	585	0.0	36.53846	2
GTAGGGA	7200	0.0	36.4375	4
TAAGGGA	6065	0.0	36.319046	4
CGACGGT	590	0.0	36.228813	28
GTAGGGT	2650	0.0	36.0	4
TAGGGTG	2280	0.0	35.921055	5
AAGGGAT	6745	0.0	35.859898	5
>>END_MODULE