FastQCFastQC Report
Thu 26 May 2016
SRR1547353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547353_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1594291
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90980.5706611904602109No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC207.034108E-445.00000410
CTACCGA207.034108E-445.00000442
TTCGCGA207.034108E-445.0000042
TACCCGT253.8914877E-545.041
CGCATCG900.045.021
TAACGCC950.045.012
CGGATTA253.8914877E-545.014
CAATCGA351.21232E-745.011
CTATGCG302.1660326E-644.9999961
CGTTTTT48950.042.839631
ATAACGC950.042.6315811
CGTAAGG3150.042.1428572
CACGACG2850.041.0526326
GCGATAT1100.040.9090929
CGACGGT2950.040.4237328
TACGGGT1400.040.178574
ACGCACG451.9290383E-840.01
ACGTGCG451.9290383E-840.01
GCGTAAG2150.039.767441
TAGGGAC17400.039.4396555
Produced by FastQC (version 0.11.5)