##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547353_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1594291
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.320761391740906	31.0	31.0	33.0	30.0	34.0
2	31.740687239657	31.0	31.0	34.0	30.0	34.0
3	31.871175964739184	31.0	31.0	34.0	30.0	34.0
4	35.637902365377464	37.0	35.0	37.0	33.0	37.0
5	35.518601685639574	37.0	35.0	37.0	33.0	37.0
6	35.556316883178795	37.0	35.0	37.0	33.0	37.0
7	35.91462286370556	37.0	35.0	37.0	35.0	37.0
8	35.88671139710379	37.0	35.0	37.0	35.0	37.0
9	37.591797231496635	39.0	37.0	39.0	35.0	39.0
10	36.95672057359666	39.0	37.0	39.0	32.0	39.0
11	36.69958997447768	39.0	35.0	39.0	32.0	39.0
12	36.361348712374344	38.0	35.0	39.0	32.0	39.0
13	36.1850985798703	38.0	35.0	39.0	32.0	39.0
14	37.27976887531825	39.0	36.0	41.0	32.0	41.0
15	37.43158808523663	39.0	36.0	41.0	32.0	41.0
16	37.50616731826248	39.0	36.0	41.0	32.0	41.0
17	37.4118840286999	39.0	35.0	41.0	32.0	41.0
18	37.29966298498831	39.0	36.0	41.0	32.0	41.0
19	37.26935735069696	39.0	35.0	41.0	32.0	41.0
20	37.124861145173625	39.0	35.0	41.0	32.0	41.0
21	37.002862087285195	39.0	35.0	41.0	31.0	41.0
22	36.92721215888442	39.0	35.0	41.0	31.0	41.0
23	36.86061515745871	39.0	35.0	41.0	31.0	41.0
24	36.74313785877233	38.0	35.0	40.0	31.0	41.0
25	36.64828378257169	38.0	35.0	40.0	31.0	41.0
26	36.54000242113893	38.0	35.0	40.0	30.0	41.0
27	36.462239327701155	38.0	35.0	40.0	30.0	41.0
28	36.54440061444241	38.0	35.0	40.0	30.0	41.0
29	36.580531408632424	38.0	35.0	40.0	30.0	41.0
30	36.509240157537114	38.0	35.0	40.0	30.0	41.0
31	36.419406494799254	38.0	35.0	41.0	30.0	41.0
32	36.32925858579143	38.0	35.0	41.0	30.0	41.0
33	36.156602527393055	38.0	35.0	41.0	30.0	41.0
34	35.96998728588444	38.0	35.0	40.0	29.0	41.0
35	35.85587574664851	38.0	35.0	40.0	29.0	41.0
36	35.712736884295275	38.0	35.0	40.0	28.0	41.0
37	35.64816586181569	38.0	35.0	40.0	27.0	41.0
38	35.5775677087809	38.0	34.0	40.0	27.0	41.0
39	35.551138405723925	38.0	34.0	40.0	27.0	41.0
40	35.377736561267675	38.0	34.0	40.0	26.0	41.0
41	35.309930872092984	38.0	34.0	40.0	26.0	41.0
42	35.25129853960161	38.0	34.0	40.0	26.0	41.0
43	35.201101931830515	38.0	34.0	40.0	26.0	41.0
44	35.08396710512698	38.0	34.0	40.0	26.0	41.0
45	34.991161588442765	38.0	34.0	40.0	25.0	41.0
46	34.91141767719946	38.0	34.0	40.0	25.0	41.0
47	34.77757699190424	37.0	34.0	40.0	24.0	41.0
48	34.722942674831636	37.0	34.0	40.0	24.0	41.0
49	34.61346140698279	37.0	33.0	40.0	24.0	41.0
50	34.54969450370102	37.0	33.0	40.0	24.0	41.0
51	34.082184494549615	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	10.0
12	7.0
13	10.0
14	26.0
15	50.0
16	125.0
17	311.0
18	633.0
19	1252.0
20	2227.0
21	3527.0
22	5253.0
23	7768.0
24	11327.0
25	16203.0
26	22088.0
27	26771.0
28	30082.0
29	34353.0
30	40608.0
31	49258.0
32	61061.0
33	76593.0
34	115701.0
35	153678.0
36	127527.0
37	164084.0
38	242545.0
39	401083.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.691778978868975	23.93640809613803	27.971179665443763	14.400633259549227
2	30.548626317278337	25.574440299794706	30.04068893319977	13.836244449727184
3	29.270754209865075	25.913462473287495	31.398220274717726	13.417563042129698
4	26.24752946607614	27.747945638531483	31.148140458674106	14.856384436718265
5	23.449483187197316	31.885960593141405	29.84869136186556	14.815864857795722
6	21.72131687377022	41.29986307393067	26.521067985706498	10.457752066592612
7	87.52781016765447	3.702209947870245	6.947226071024675	1.8227538134506185
8	88.12450173776305	3.3213509955208926	6.5010089124256485	2.0531383542904025
9	83.22395346897147	5.724237294195351	8.160116315026555	2.891692921806621
10	37.717518320055746	34.7493650782699	14.78832910679418	12.74478749488017
11	30.938705669165795	25.945012547897466	25.89370447427728	17.222577308659464
12	30.728016403529846	22.995362828994203	28.104091411166472	18.172529356309482
13	24.56897768349693	27.16379882969922	28.773166253839484	19.49405723296437
14	19.05442607403542	31.115712250774795	29.874219951062887	19.9556417241269
15	18.442429895169703	28.229288128704233	35.67585842233319	17.652423553792875
16	22.2350248480359	26.422215266849026	33.678920598560744	17.663839286554335
17	22.19506978337079	26.015451382464054	28.442486346595448	23.34699248756971
18	22.622281628636177	27.656557052633428	31.286446451745636	18.434714866984763
19	25.618597859487384	28.70260197166013	27.12026850807036	18.55853166078213
20	26.440781513537992	27.94489839056985	27.552498257846274	18.061821838045876
21	25.294378504300656	27.086460376430647	29.95814440400153	17.661016715267163
22	23.82915038722542	24.330752666859436	30.04288426642313	21.797212679492013
23	21.201587414091907	27.343565258789017	30.356001507880304	21.098845819238772
24	21.109069799678977	26.0385337432125	33.22574109745335	19.62665535965517
25	21.804614088645046	27.853509804671795	30.608841171404716	19.73303493527844
26	21.015172261525656	31.463892099999306	27.268986653001242	20.251948985473796
27	20.03749629145495	30.871716643950194	30.007194420591976	19.083592644002884
28	18.14700076711215	29.485457799109447	32.88333183841595	19.484209595362454
29	20.153974399905664	27.789092455517846	31.796140102402887	20.260793042173606
30	20.642655575425064	27.895158412109204	31.076698043205415	20.385487969260314
31	23.829903072902	27.043494569059224	28.83212663183823	20.29447572620055
32	23.992232283817696	27.8408395957827	28.906077999562186	19.260850120837414
33	23.67967955661796	27.515679383500252	28.15514858956113	20.649492470320663
34	20.776131835405206	28.225650147934093	28.992825023788004	22.005392992872693
35	20.63406241395078	27.731888344097783	29.475171094862855	22.158878147088583
36	23.285585881122078	27.945023838182614	28.076932003003215	20.69245827769209
37	21.057322659414123	29.91009796831319	28.540523655969956	20.492055716302733
38	20.99165083413254	29.882185874473354	27.5845501229073	21.541613168486805
39	21.107250809293912	28.52753982804896	28.00498779708347	22.360221565573664
40	22.312175129885322	27.11732048917042	29.066400048673675	21.504104332270582
41	19.3753837912903	26.65360338859092	29.758808147320657	24.212204672798126
42	21.393145918781453	27.82296331096393	28.37474463570327	22.409146134551346
43	20.9056564955833	27.897604640558093	28.422665623778848	22.77407324007976
44	20.788739320487917	27.507211669638732	29.850008561799573	21.854040448073782
45	20.699608791619596	29.524660178097978	27.63209476814459	22.143636262137843
46	22.317318482008616	28.63454664173604	27.806216054660034	21.241918821595306
47	21.2538990686142	28.42203838571503	28.66653578299068	21.65752676268009
48	21.16044059710555	27.266352253133213	29.784838526969043	21.788368622792202
49	20.920459313889374	27.017652360829985	30.118780072144922	21.94310825313572
50	20.32113334391275	28.61234241427694	29.350852510614434	21.715671731195872
51	19.360455525371467	29.43101353517018	28.302925877396284	22.90560506206207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1437.0
1	2023.0
2	2609.0
3	9097.0
4	15585.0
5	11147.0
6	6709.0
7	6890.5
8	7072.0
9	7607.5
10	8143.0
11	7966.5
12	7790.0
13	7577.5
14	7365.0
15	6862.0
16	6359.0
17	6682.0
18	7005.0
19	7088.5
20	7172.0
21	7329.0
22	7486.0
23	8426.0
24	9366.0
25	11453.5
26	16268.0
27	18995.0
28	21693.5
29	24392.0
30	29223.0
31	34054.0
32	38753.0
33	43452.0
34	50192.0
35	56932.0
36	60479.5
37	64027.0
38	70423.5
39	76820.0
40	86008.0
41	95196.0
42	105096.0
43	114996.0
44	119010.5
45	123025.0
46	123251.5
47	123478.0
48	123950.0
49	124422.0
50	122200.0
51	119978.0
52	111668.5
53	103359.0
54	94141.0
55	84923.0
56	77770.5
57	70618.0
58	66225.5
59	61833.0
60	56348.0
61	50863.0
62	42640.0
63	34417.0
64	29148.5
65	23880.0
66	19120.0
67	14360.0
68	11896.5
69	9433.0
70	7521.0
71	5609.0
72	4509.5
73	3410.0
74	2726.5
75	1554.0
76	1065.0
77	853.5
78	642.0
79	406.5
80	171.0
81	161.5
82	152.0
83	99.5
84	47.0
85	35.5
86	24.0
87	17.5
88	11.0
89	13.0
90	15.0
91	9.0
92	3.0
93	3.5
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1594291.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.556352917313063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.52826847845478	16.759238595805666
2	6.721615616491143	2.7634380557422946
3	2.095359209855256	1.2921883021898066
4	1.044958185146509	0.8592211695082641
5	0.6663254439649826	0.6848610491964746
6	0.47785933167450273	0.589382704003946
7	0.3642371871910016	0.5241171715855354
8	0.2939575184815281	0.4834155594083095
9	0.24413102051606622	0.4516599074212847
>10	3.6407338445075808	19.100063224121296
>50	1.9219905991802364	28.69001021684168
>100	0.9974962329468687	26.768395426326023
>500	0.0018403989537744702	0.24565507501318834
>1k	9.201994768872351E-4	0.21469597803385684
>5k	3.067331589624117E-4	0.5736575648023582
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9098	0.5706611904602109	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04942635942873666	0.0
2	0.0	0.0	0.0	0.17085964858360236	0.0
3	0.0	0.0	0.0	0.2374723309609099	0.0
4	0.0	0.0	0.0	0.3593446867604471	0.0
5	0.0	0.0	0.0	0.6362702919354121	0.0
6	0.0	0.0	0.0	0.9036618785403668	0.0
7	0.0	0.0	0.0	1.0587151279157945	0.0
8	0.0	0.0	0.0	1.3898968256108828	0.0
9	0.0	0.0	0.0	1.491258496723622	0.0
10	0.0	0.0	0.0	1.7299853038121649	0.0
11	0.0	0.0	0.0	2.021149213035763	0.0
12	0.0	0.0	0.0	2.2807003238430124	0.0
13	0.0	0.0	0.0	2.3798039379260123	0.0
14	0.0	0.0	0.0	2.4197590025911206	0.0
15	0.0	0.0	0.0	2.481541951877041	0.0
16	0.0	0.0	0.0	2.6198479449485697	0.0
17	0.0	0.0	0.0	2.7767829085154467	0.0
18	0.0	0.0	0.0	2.9633235086944603	0.0
19	0.0	0.0	0.0	3.0769163220516202	0.0
20	0.0	0.0	0.0	3.1982241635937227	0.0
21	0.0	0.0	0.0	3.347067756137368	0.0
22	0.0	0.0	0.0	3.5117804716955687	0.0
23	0.0	0.0	0.0	3.6791903109281807	0.0
24	0.0	0.0	0.0	3.81486190413168	0.0
25	0.0	0.0	0.0	3.931590907807922	0.0
26	0.0	0.0	0.0	4.036966902529087	0.0
27	0.0	0.0	0.0	4.153319563367039	0.0
28	0.0	0.0	0.0	4.277638147615461	0.0
29	0.0	0.0	0.0	4.4117416456594185	0.0
30	0.0	0.0	0.0	4.574384475607025	0.0
31	0.0	0.0	0.0	4.720217325444351	0.0
32	0.0	0.0	0.0	4.850557395105411	0.0
33	0.0	0.0	0.0	4.985789921664238	0.0
34	0.0	0.0	0.0	5.125789457508071	0.0
35	0.0	0.0	0.0	5.282348078236658	0.0
36	0.0	0.0	0.0	5.423539366401743	0.0
37	0.0	0.0	0.0	5.5758954921027595	0.0
38	0.0	0.0	0.0	5.7329559032824	0.0
39	0.0	0.0	0.0	5.956189930194676	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAC	20	7.034108E-4	45.000004	10
CTACCGA	20	7.034108E-4	45.000004	42
TTCGCGA	20	7.034108E-4	45.000004	2
TACCCGT	25	3.8914877E-5	45.0	41
CGCATCG	90	0.0	45.0	21
TAACGCC	95	0.0	45.0	12
CGGATTA	25	3.8914877E-5	45.0	14
CAATCGA	35	1.21232E-7	45.0	11
CTATGCG	30	2.1660326E-6	44.999996	1
CGTTTTT	4895	0.0	42.83963	1
ATAACGC	95	0.0	42.63158	11
CGTAAGG	315	0.0	42.142857	2
CACGACG	285	0.0	41.05263	26
GCGATAT	110	0.0	40.909092	9
CGACGGT	295	0.0	40.42373	28
TACGGGT	140	0.0	40.17857	4
ACGCACG	45	1.9290383E-8	40.0	1
ACGTGCG	45	1.9290383E-8	40.0	1
GCGTAAG	215	0.0	39.76744	1
TAGGGAC	1740	0.0	39.439655	5
>>END_MODULE