FastQCFastQC Report
Thu 26 May 2016
SRR1547352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547352_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1420812
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478163.365399503945631No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17220.12119830069002796No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG351.2121927E-745.0000041
CGTGGTA207.03385E-445.041
ATATCGA253.8912724E-545.041
CGTAGCA253.8912724E-545.028
TACGACT207.03385E-445.032
CATGTCG253.8912724E-545.01
CAGTCGT253.8912724E-545.029
CCGAACG207.03385E-445.042
CGGTCCG207.03385E-445.029
TATCGTG207.03385E-445.01
AATCGCG207.03385E-445.01
ACCGCGT302.1658652E-644.99999623
TATTACG302.1658652E-644.9999961
CGTTTTT184500.044.231711
CGACGGT2800.044.1964328
TCACGAC2900.042.67241325
ACGGGCC850.042.3529435
CACGACG2950.041.94915426
TACGGGA5900.041.9491544
CGGTCTA2950.041.94915431
Produced by FastQC (version 0.11.5)