##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547352_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1420812
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.462819148486922	31.0	31.0	33.0	30.0	34.0
2	31.865821797676258	31.0	31.0	34.0	30.0	34.0
3	31.764523385219157	31.0	31.0	34.0	30.0	34.0
4	35.63211248215809	37.0	35.0	37.0	33.0	37.0
5	35.58730219057835	37.0	35.0	37.0	33.0	37.0
6	35.65029011579294	37.0	35.0	37.0	33.0	37.0
7	36.055185344718375	37.0	35.0	37.0	35.0	37.0
8	36.07016832628103	37.0	35.0	37.0	35.0	37.0
9	37.816525339031486	39.0	38.0	39.0	35.0	39.0
10	37.24112197813645	39.0	37.0	39.0	34.0	39.0
11	36.91044205707722	39.0	37.0	39.0	32.0	39.0
12	36.71037617925524	39.0	35.0	39.0	32.0	39.0
13	36.60850344732449	39.0	35.0	39.0	32.0	39.0
14	37.79642415745362	40.0	36.0	41.0	33.0	41.0
15	37.91658713468073	40.0	36.0	41.0	33.0	41.0
16	37.97833844308747	40.0	36.0	41.0	33.0	41.0
17	37.89746567455793	40.0	36.0	41.0	33.0	41.0
18	37.65267396390233	39.0	36.0	41.0	33.0	41.0
19	37.45842518221974	39.0	36.0	41.0	33.0	41.0
20	37.15500432147251	39.0	35.0	41.0	32.0	41.0
21	37.04059650397097	38.0	35.0	41.0	32.0	41.0
22	36.974227413619815	38.0	35.0	41.0	32.0	41.0
23	36.919346120387495	38.0	35.0	40.0	32.0	41.0
24	36.7707163227788	38.0	35.0	40.0	32.0	41.0
25	36.69166786316557	38.0	35.0	40.0	32.0	41.0
26	36.55289369740684	38.0	35.0	40.0	31.0	41.0
27	36.45896994113225	38.0	35.0	40.0	31.0	41.0
28	36.42374219812333	38.0	35.0	40.0	31.0	41.0
29	36.38376787358215	38.0	35.0	40.0	31.0	41.0
30	36.2399888232926	38.0	35.0	40.0	31.0	41.0
31	36.02262579426412	38.0	35.0	40.0	30.0	41.0
32	35.70773473197017	38.0	35.0	40.0	29.0	41.0
33	35.27975200096846	38.0	34.0	40.0	26.0	41.0
34	34.8300457766404	38.0	34.0	40.0	23.0	41.0
35	34.48989662249474	38.0	34.0	40.0	21.0	41.0
36	34.26422566813906	38.0	33.0	40.0	21.0	41.0
37	34.14472639589193	38.0	33.0	40.0	20.0	41.0
38	34.044080427248645	37.0	33.0	40.0	18.0	41.0
39	33.99842062144745	37.0	33.0	40.0	18.0	41.0
40	33.8476131958345	37.0	33.0	40.0	18.0	41.0
41	33.74990146479619	37.0	33.0	40.0	18.0	41.0
42	33.66848464117702	37.0	33.0	40.0	18.0	41.0
43	33.61221048245651	37.0	33.0	40.0	18.0	41.0
44	33.477287635521094	37.0	33.0	40.0	17.0	41.0
45	33.37731029861797	36.0	33.0	40.0	17.0	41.0
46	33.300702696767765	36.0	33.0	40.0	17.0	41.0
47	33.190546673310756	36.0	33.0	40.0	15.0	41.0
48	33.121389036691696	36.0	33.0	40.0	15.0	41.0
49	33.01423059489925	36.0	32.0	40.0	15.0	41.0
50	32.93348240302024	36.0	32.0	40.0	15.0	41.0
51	32.5735262652624	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	5.0
14	12.0
15	46.0
16	104.0
17	258.0
18	652.0
19	1330.0
20	2379.0
21	3881.0
22	6103.0
23	9512.0
24	15238.0
25	23878.0
26	34359.0
27	38154.0
28	35518.0
29	34166.0
30	35693.0
31	41276.0
32	50020.0
33	63371.0
34	95250.0
35	139289.0
36	129168.0
37	144111.0
38	195577.0
39	321367.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.620825274561305	22.620726739357494	26.970492929395302	22.787955056685895
2	37.045224843258644	24.22107921385799	27.340211090559485	11.393484852323882
3	25.35078532557439	23.709892652933675	39.727775384779974	11.211546636711965
4	22.6357885490832	25.65779286774042	39.25445449503523	12.451964088141148
5	20.48659498934412	29.260521448298576	37.70745179517065	12.54543176718665
6	19.07620431133746	37.1348918787285	34.26104227723302	9.527861532701019
7	81.1214291545961	2.7454019250963535	15.083698617410327	1.0494703028972165
8	81.92456144796074	2.3660413904161843	14.639234465925119	1.0701626956979529
9	77.50497602779257	5.4353426069036574	15.483892309468105	1.575789055835677
10	33.238598773095944	38.68851051370625	20.15938773039642	7.913502982801384
11	23.020709284549962	24.47431468765748	37.29536349636687	15.209612531425693
12	24.762459776522157	23.273663229195698	36.545158683907516	15.41871831037463
13	20.02235341480787	27.506031762119125	36.4964541403085	15.975160682764503
14	14.856363825755976	31.077862518053056	36.848084053344145	17.21768960284682
15	14.32624442924187	30.15043510330712	40.89774016548284	14.625580301968169
16	18.36372440548081	27.888137206048373	39.95088723912804	13.79725114934277
17	17.579313800840644	27.994695990743324	35.04847932027601	19.37751088814002
18	17.360917559817906	30.074703760947962	37.04726592962334	15.517112749610787
19	20.536918325577204	29.977787349769002	33.98260994417277	15.502684380481021
20	21.561614062944287	30.256290065117692	33.60620546560699	14.575890406331027
21	20.77769613432319	29.191265276475704	35.66742116479872	14.363617424402383
22	19.325709523849742	26.67298699616839	35.610552275740915	18.390751204240953
23	16.367612323094118	30.8534837825131	35.7125362116874	17.066367682705383
24	16.180888111868423	30.58624223331447	38.41767946779729	14.815190187019816
25	17.157442363943996	31.67343744281439	35.487383270974625	15.681736922266987
26	16.551802771936046	34.71296695129264	32.63021427183892	16.10501600493239
27	15.622404653113852	33.374014296050426	35.404543317483245	15.599037733352478
28	14.13705683792085	32.176389275991475	38.27114354326962	15.415410342818051
29	15.62557185609356	30.598136840060477	37.64551538134531	16.130775922500654
30	16.365148942998793	31.77689940681807	36.02052910589156	15.837422544291574
31	19.46147695824641	31.24431663020864	32.95622503188317	16.33798137966177
32	19.71337516856558	31.649366700168635	33.50907790756272	15.128180223703067
33	19.528340132262397	31.972773315540692	31.9765035768279	16.522382975369016
34	17.587548528587877	32.0142284834306	32.75077913193301	17.647443856048515
35	17.78757499232833	31.855094129272555	33.031745227377016	17.32558565102209
36	20.203939718977598	32.87113284516178	30.95201898632613	15.972908449534492
37	17.621261644749623	34.35943671646917	31.475170536284885	16.54413110249632
38	17.612604623271764	35.35154545428952	30.075759495274536	16.960090427164186
39	17.9750030264384	34.55728132926805	30.017342195871095	17.45037344842245
40	19.78530586734909	32.83727896442316	30.660425165327997	16.716990002899752
41	17.05749951436221	32.632114593626746	31.809204877211062	18.50118101479999
42	18.026311714709617	33.69650594167279	29.313589693780738	18.963592649836855
43	18.388780500164696	33.59853379616726	29.335548967773356	18.67713673589469
44	18.221833711990048	34.518711835204094	29.72743754979547	17.53201690301039
45	17.461916143726263	35.62300994079442	28.496310560440087	18.418763355039232
46	18.81037040790759	35.17727890811733	29.0740083839382	16.93834230003688
47	18.832822357919273	33.31334476341697	30.088639454058665	17.765193424605087
48	18.459092406314138	33.160333668353026	31.245935422842713	17.134638502490127
49	18.19304735601895	32.85248153872574	30.809987528258485	18.144483576996816
50	17.227331976362812	34.423484598947645	30.706595946543246	17.6425874781463
51	16.682643446142066	34.679324217419335	30.066257886335418	18.57177445010318
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2138.0
1	2344.0
2	2550.0
3	32428.0
4	62306.0
5	40074.5
6	17843.0
7	18166.5
8	18490.0
9	21035.5
10	23581.0
11	26101.5
12	28622.0
13	29228.5
14	29835.0
15	29222.0
16	28609.0
17	26463.5
18	24318.0
19	22476.0
20	20634.0
21	19314.0
22	17994.0
23	18217.5
24	18441.0
25	19191.5
26	21618.0
27	23294.0
28	26083.5
29	28873.0
30	33058.0
31	37243.0
32	43046.0
33	48849.0
34	50837.5
35	52826.0
36	56144.5
37	59463.0
38	63465.0
39	67467.0
40	75926.0
41	84385.0
42	86868.5
43	89352.0
44	92100.5
45	94849.0
46	93481.0
47	92113.0
48	93314.0
49	94515.0
50	88616.0
51	82717.0
52	75567.5
53	68418.0
54	60246.5
55	52075.0
56	45271.0
57	38467.0
58	35282.0
59	32097.0
60	27634.0
61	23171.0
62	19223.0
63	15275.0
64	12090.0
65	8905.0
66	6961.0
67	5017.0
68	3709.0
69	2401.0
70	1992.0
71	1583.0
72	1341.5
73	1100.0
74	789.5
75	349.0
76	219.0
77	184.0
78	149.0
79	113.0
80	77.0
81	85.5
82	94.0
83	57.5
84	21.0
85	14.5
86	8.0
87	5.5
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1420812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.850442138502572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1718719808009	20.720988833404228
2	6.972751211421832	3.744429058969113
3	3.3781285355548976	2.7211273434102363
4	2.158194716190739	2.3179392940280574
5	1.5715045452741314	2.109779593163843
6	1.0796190491222566	1.739294928604939
7	0.7898134833293543	1.4844788864040797
8	0.5812271808574057	1.2484965431149393
9	0.4483434984546087	1.083439904708636
>10	4.415467700155101	26.856473612018632
>50	1.1431010797951398	21.558489988302643
>100	0.28603354191008196	10.189650935772518
>500	0.0028918832312527054	0.5415619003185622
>1k	7.886954267052833E-4	0.3085462807993268
>5k	0.0	0.0
>10k+	2.6289847556842775E-4	3.375302896980236
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47816	3.365399503945631	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1722	0.12119830069002796	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03237585268142443	0.0
2	0.0	0.0	0.0	0.11423045413467792	0.0
3	0.0	0.0	0.0	0.20987998412175574	0.0
4	0.0	0.0	0.0	0.3059518078394608	0.0
5	0.0	0.0	0.0	0.5260372237847091	0.0
6	0.0	0.0	0.0	0.8609161521721382	0.0
7	0.0	0.0	0.0	1.0582680889519513	0.0
8	0.0	0.0	0.0	1.3546479055638607	0.0
9	0.0	0.0	0.0	1.532152037004192	0.0
10	0.0	0.0	0.0	1.7520263060841266	0.0
11	0.0	0.0	0.0	2.039045278333798	0.0
12	0.0	0.0	0.0	2.2517405539930686	0.0
13	7.038228843787919E-5	0.0	0.0	2.3694901225496405	0.0
14	7.038228843787919E-5	0.0	0.0	2.433538005028111	0.0
15	7.038228843787919E-5	0.0	0.0	2.500401179044096	0.0
16	7.038228843787919E-5	0.0	0.0	2.5890828624758235	0.0
17	7.038228843787919E-5	0.0	0.0	2.7050024915330106	0.0
18	7.038228843787919E-5	0.0	0.0	2.8436555997556328	0.0
19	7.038228843787919E-5	0.0	0.0	2.9339560758214316	0.0
20	7.038228843787919E-5	0.0	0.0	3.0259457268097396	0.0
21	7.038228843787919E-5	0.0	0.0	3.1315191594665586	0.0
22	7.038228843787919E-5	0.0	0.0	3.241948970025591	0.0
23	7.038228843787919E-5	0.0	0.0	3.362513830119678	0.0
24	7.038228843787919E-5	0.0	0.0	3.459148712144886	0.0
25	7.038228843787919E-5	0.0	0.0	3.5400883438484474	0.0
26	7.038228843787919E-5	0.0	0.0	3.618071919437617	0.0
27	7.038228843787919E-5	0.0	0.0	3.7005599614868117	0.0
28	7.038228843787919E-5	0.0	0.0	3.7882562928804093	0.0
29	7.038228843787919E-5	0.0	0.0	3.8834835291368597	0.0
30	7.038228843787919E-5	0.0	0.0	4.006159857884083	0.0
31	1.4076457687575838E-4	0.0	0.0	4.106736148061812	0.0
32	1.4076457687575838E-4	0.0	0.0	4.207453202816418	0.0
33	1.4076457687575838E-4	0.0	0.0	4.304158467130064	0.0
34	1.4076457687575838E-4	0.0	0.0	4.401074878309023	0.0
35	1.4076457687575838E-4	0.0	0.0	4.519598652038412	0.0
36	1.4076457687575838E-4	0.0	0.0	4.630380374039634	0.0
37	1.4076457687575838E-4	0.0	0.0	4.740528655444915	0.0
38	1.4076457687575838E-4	0.0	0.0	4.863486513345889	0.0
39	1.4076457687575838E-4	0.0	0.0	5.008685174393234	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	35	1.2121927E-7	45.000004	1
CGTGGTA	20	7.03385E-4	45.0	41
ATATCGA	25	3.8912724E-5	45.0	41
CGTAGCA	25	3.8912724E-5	45.0	28
TACGACT	20	7.03385E-4	45.0	32
CATGTCG	25	3.8912724E-5	45.0	1
CAGTCGT	25	3.8912724E-5	45.0	29
CCGAACG	20	7.03385E-4	45.0	42
CGGTCCG	20	7.03385E-4	45.0	29
TATCGTG	20	7.03385E-4	45.0	1
AATCGCG	20	7.03385E-4	45.0	1
ACCGCGT	30	2.1658652E-6	44.999996	23
TATTACG	30	2.1658652E-6	44.999996	1
CGTTTTT	18450	0.0	44.23171	1
CGACGGT	280	0.0	44.19643	28
TCACGAC	290	0.0	42.672413	25
ACGGGCC	85	0.0	42.352943	5
CACGACG	295	0.0	41.949154	26
TACGGGA	590	0.0	41.949154	4
CGGTCTA	295	0.0	41.949154	31
>>END_MODULE