##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547351_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	891302
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.328260230539144	31.0	31.0	33.0	30.0	34.0
2	31.744940547648273	31.0	31.0	34.0	30.0	34.0
3	31.824832660534813	31.0	31.0	34.0	30.0	34.0
4	35.60429349423652	37.0	35.0	37.0	33.0	37.0
5	35.486783379819634	37.0	35.0	37.0	33.0	37.0
6	35.52830017210777	37.0	35.0	37.0	33.0	37.0
7	35.929920498327164	37.0	35.0	37.0	35.0	37.0
8	35.918528175635196	37.0	35.0	37.0	35.0	37.0
9	37.65289542713917	39.0	37.0	39.0	35.0	39.0
10	36.987255722527266	39.0	37.0	39.0	32.0	39.0
11	36.752263542547865	39.0	35.0	39.0	32.0	39.0
12	36.14074354147079	38.0	35.0	39.0	32.0	39.0
13	35.778000049365986	38.0	35.0	39.0	31.0	39.0
14	36.843626514918626	39.0	35.0	41.0	31.0	41.0
15	37.10028475196959	39.0	35.0	41.0	32.0	41.0
16	37.221392973425395	39.0	35.0	41.0	32.0	41.0
17	37.155865239840146	39.0	35.0	41.0	32.0	41.0
18	37.03936039636397	38.0	35.0	41.0	32.0	41.0
19	36.94142950425333	38.0	35.0	41.0	32.0	41.0
20	36.74856670354156	38.0	35.0	40.0	31.0	41.0
21	36.60394681039648	38.0	35.0	40.0	31.0	41.0
22	36.482224880007	38.0	35.0	40.0	31.0	41.0
23	36.43585114809571	38.0	35.0	40.0	31.0	41.0
24	36.297844052857506	38.0	35.0	40.0	31.0	41.0
25	36.27541731085535	38.0	35.0	40.0	31.0	41.0
26	36.14370550049254	38.0	35.0	40.0	30.0	41.0
27	36.0757543458895	38.0	35.0	40.0	30.0	41.0
28	36.14013207644547	38.0	35.0	40.0	30.0	41.0
29	36.214911444156975	38.0	35.0	40.0	30.0	41.0
30	36.15873968643625	38.0	35.0	40.0	30.0	41.0
31	35.98792889503221	38.0	35.0	40.0	30.0	41.0
32	35.8135895577481	38.0	35.0	40.0	30.0	41.0
33	35.55565117098357	38.0	34.0	40.0	29.0	41.0
34	35.340552360479386	38.0	34.0	40.0	27.0	41.0
35	35.17081527922073	38.0	34.0	40.0	26.0	41.0
36	34.99986873136154	38.0	34.0	40.0	25.0	41.0
37	34.898261195419735	38.0	34.0	40.0	25.0	41.0
38	34.86821862847834	37.0	34.0	40.0	25.0	41.0
39	34.87586138031778	37.0	34.0	40.0	25.0	41.0
40	34.73782062645434	37.0	34.0	40.0	24.0	41.0
41	34.68704210245237	37.0	34.0	40.0	24.0	41.0
42	34.592693610022195	37.0	34.0	40.0	23.0	41.0
43	34.51436213539294	37.0	33.0	40.0	23.0	41.0
44	34.377249237632135	37.0	33.0	40.0	23.0	41.0
45	34.27838039183128	37.0	33.0	40.0	23.0	41.0
46	34.19202357898894	36.0	33.0	40.0	23.0	41.0
47	34.0482911515962	36.0	33.0	40.0	23.0	41.0
48	34.03676307244907	36.0	33.0	40.0	23.0	41.0
49	33.968455136418406	36.0	33.0	40.0	23.0	41.0
50	33.885926431220845	36.0	33.0	40.0	23.0	41.0
51	33.445163367747405	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	4.0
14	7.0
15	30.0
16	82.0
17	217.0
18	473.0
19	846.0
20	1447.0
21	2301.0
22	3649.0
23	5254.0
24	7691.0
25	11361.0
26	15499.0
27	18410.0
28	19826.0
29	21679.0
30	24497.0
31	28801.0
32	35695.0
33	45101.0
34	73762.0
35	108862.0
36	63166.0
37	78912.0
38	119692.0
39	203964.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.725163861407246	24.225346739937752	28.876519967418453	16.17296943123655
2	31.088564818658547	25.933858557481077	30.932276602094465	12.045300021765911
3	27.691960749555143	24.899192417384906	33.81547444076194	13.593372392298008
4	24.832099557725666	27.130422685015855	33.91061615479377	14.126861602464707
5	21.799120836708546	31.81301062939385	33.329443892193666	13.058424641703933
6	20.871376929480693	41.39124561596406	28.353240540243373	9.384136914311872
7	87.59129902098279	3.2709452015141895	7.983152736109646	1.1546030413933772
8	88.590511409152	2.5218163989310023	7.482200197015152	1.4054719949018402
9	84.31530502568152	5.638605096813426	8.48163697601935	1.5644529014856918
10	37.36141061054502	38.29835454200708	14.301549867497213	10.038684979950679
11	35.0354874105522	22.432576163859164	26.02047341978364	16.51146300580499
12	34.56090079456794	21.417207635571334	26.76702172776455	17.25486984209617
13	21.921974818860498	32.743783812893945	27.170139862807442	18.16410150543811
14	15.697373056494882	36.50524737967603	29.76948329522429	18.027896268604803
15	14.819556110050241	28.146127799556155	40.85483932494261	16.179476765450993
16	18.124384327646524	24.094302492309005	40.9808347787843	16.80047840126018
17	18.193160118568116	24.51896214751005	29.91679587838914	27.37108185553269
18	21.296373170934206	26.80393402011888	32.41987564259926	19.479817166347658
19	24.740211510801053	28.398230902657012	27.91253694034121	18.949020646200726
20	26.614996937065104	26.66122144907113	29.387345703252098	17.336435910611666
21	24.124258668778932	27.43795032435695	30.60242207467278	17.835368932191333
22	23.37322254409841	24.015541309230766	30.509748659825735	22.101487486845087
23	19.62982243953228	28.209181624185742	30.283450502747666	21.877545433534312
24	18.84052767748754	26.739870436731884	36.68835030102031	17.731251584760273
25	17.826729885044575	28.199981599951528	34.34032460378189	19.63296391122201
26	16.83121994565254	35.02314591462826	27.77274145014821	20.37289268957099
27	17.67807095686984	33.493024810894624	30.906359460654187	17.92254477158135
28	14.228622846128472	31.091481899513298	36.99228768700171	17.68760756735652
29	16.39208708159524	26.234991058025226	37.659289443981955	19.713632416397584
30	18.591566046076412	29.449726355376743	34.24641703934245	17.712290559204398
31	25.512340373969767	28.269654954213053	27.618136164846486	18.5998685069707
32	25.35493020323078	27.883253936376224	29.408214050905308	17.353601809487692
33	23.99321442115018	28.176083976026085	27.63317035079019	20.197531252033542
34	20.08410168495078	28.292542819381083	29.48899475149837	22.134360744169765
35	19.888769463100047	27.096988450603725	31.207267570363356	21.806974515932872
36	25.755916625341353	26.59255785356703	29.171032938330665	18.48049258276095
37	19.348099746213965	30.670188106836964	31.160930862939836	18.820781284009236
38	18.928376689382496	32.89995983404054	27.750302366650136	20.421361109926824
39	18.995132962789267	30.85407639610368	29.661551303598554	20.4892393375085
40	22.076019127074773	27.51009197780326	28.703514633648304	21.710374261473664
41	17.787237098087967	25.508525729775094	31.011823153095136	25.692414019041802
42	20.443014825502466	27.237793699554135	26.788451052505213	25.530740422438186
43	20.478468577429425	27.73145353651175	27.701385164624337	24.088692721434484
44	19.190689575474977	30.387680045596216	29.18988176846905	21.231748610459753
45	19.281006886554724	32.86282315085123	26.348757211360457	21.50741275123359
46	22.88550906426778	31.951347579159478	27.245422987943478	17.917720368629265
47	20.896396507581045	28.905578580548458	28.27313301215525	21.92489189971525
48	21.360436754321206	27.772180473060757	30.831300726353135	20.0360820462649
49	19.955750127341798	26.741104586324276	31.377916800366208	21.92522848596772
50	18.552073259119805	29.84252251201052	32.14017246679577	19.46523176207391
51	18.03204749905195	30.5498024238698	28.846339400113543	22.57181067696471
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1218.0
1	1413.0
2	1608.0
3	8328.0
4	15048.0
5	10224.5
6	5401.0
7	5510.5
8	5620.0
9	5863.0
10	6106.0
11	6171.5
12	6237.0
13	5931.5
14	5626.0
15	5427.0
16	5228.0
17	4685.0
18	4142.0
19	4441.0
20	4740.0
21	5157.0
22	5574.0
23	5650.5
24	5727.0
25	5907.0
26	8277.0
27	10467.0
28	12024.0
29	13581.0
30	15817.5
31	18054.0
32	20979.5
33	23905.0
34	27263.0
35	30621.0
36	31937.5
37	33254.0
38	36870.0
39	40486.0
40	49609.5
41	58733.0
42	69691.0
43	80649.0
44	80109.0
45	79569.0
46	81459.0
47	83349.0
48	78108.5
49	72868.0
50	68346.5
51	63825.0
52	59229.0
53	54633.0
54	47692.0
55	40751.0
56	36392.0
57	32033.0
58	28204.5
59	24376.0
60	21614.0
61	18852.0
62	15879.5
63	12907.0
64	10638.5
65	8370.0
66	6776.0
67	5182.0
68	3841.5
69	2501.0
70	1906.5
71	1312.0
72	1353.0
73	1394.0
74	917.5
75	325.5
76	210.0
77	217.5
78	225.0
79	194.5
80	164.0
81	108.5
82	53.0
83	60.5
84	68.0
85	48.0
86	28.0
87	25.5
88	23.0
89	25.0
90	27.0
91	22.0
92	17.0
93	14.5
94	12.0
95	6.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	891302.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.39042700973737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.30876660590907	16.98701324749035
2	7.006050737092832	2.8571273236241614
3	2.0110467553306752	1.2301830623321788
4	0.966697919672568	0.7884553348619381
5	0.6035625546602952	0.6153449108305684
6	0.3989864737581334	0.48813027426226263
7	0.28951106704717994	0.4132277996795693
8	0.23824336104679786	0.3886307091183391
9	0.20999964783685265	0.3853784242159106
>10	2.429304338125342	11.941735257286954
>50	1.1497903555221511	17.198164716058074
>100	1.3825123457504327	44.748975127455026
>500	0.00331670294862159	0.452888798927267
>1k	0.001658351474310795	0.3729738764684736
>5k	0.0	0.0
>10k+	5.527838247702649E-4	1.1317711373889219
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10041	1.1265541870207851	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1162	0.13037107512380763	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1107	0.1242003271618374	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1040	0.11668323418998275	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03747326944178292	0.0
2	0.0	0.0	0.0	0.12184422339453968	0.0
3	0.0	0.0	0.0	0.1712102070903016	0.0
4	0.0	0.0	0.0	0.3186349856726452	0.0
5	0.0	0.0	0.0	0.541118498556045	0.0
6	0.0	0.0	0.0	0.778299611130683	0.0
7	0.0	0.0	0.0	0.908782881671981	0.0
8	0.0	0.0	0.0	1.1848958041157767	0.0
9	0.0	0.0	0.0	1.2605155155042846	0.0
10	0.0	0.0	0.0	1.4352037805367877	0.0
11	0.0	0.0	0.0	1.6757507556361368	0.0
12	0.0	0.0	0.0	1.8920635205575664	0.0
13	0.0	0.0	0.0	1.9639807831688922	0.0
14	0.0	0.0	0.0	1.989785729191677	0.0
15	0.0	0.0	0.0	2.0338785282653915	0.0
16	0.0	0.0	0.0	2.1503373716203935	0.0
17	0.0	0.0	0.0	2.282166987171576	0.0
18	0.0	0.0	0.0	2.4439527791926867	0.0
19	0.0	0.0	0.0	2.5347188719423945	0.0
20	0.0	0.0	0.0	2.640406955218321	0.0
21	0.0	0.0	0.0	2.787382952130703	0.0
22	0.0	0.0	0.0	2.9435589732772955	0.0
23	0.0	0.0	0.0	3.103774029453541	0.0
24	0.0	0.0	0.0	3.22236458574086	0.0
25	0.0	0.0	0.0	3.316608736432769	0.0
26	0.0	0.0	0.0	3.4128724046395047	0.0
27	0.0	0.0	0.0	3.5066677736614524	0.0
28	0.0	0.0	0.0	3.616843673636994	0.0
29	0.0	0.0	0.0	3.730609826972227	0.0
30	0.0	0.0	0.0	3.8725370300975426	0.0
31	0.0	0.0	0.0	4.005264208988648	0.0
32	0.0	0.0	0.0	4.124079156110948	0.0
33	0.0	0.0	0.0	4.243118494068228	0.0
34	0.0	0.0	0.0	4.3720310287646615	0.0
35	0.0	0.0	0.0	4.529889981173609	0.0
36	0.0	0.0	0.0	4.674061092648732	0.0
37	0.0	0.0	0.0	4.801066305247828	0.0
38	1.1219541749036802E-4	0.0	0.0	4.939178864178471	0.0
39	1.1219541749036802E-4	0.0	0.0	5.090867068625449	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	55	1.8189894E-12	45.000004	6
TCGTTAG	35	1.2115197E-7	45.000004	1
ACCGCTC	35	1.2115197E-7	45.000004	18
ATAGCGG	130	0.0	45.000004	2
AACGACC	45	3.8380676E-10	45.000004	36
ACGCTAC	45	3.8380676E-10	45.000004	16
CGTAAGG	110	0.0	45.000004	2
ATACGTA	45	3.8380676E-10	45.000004	10
TGTGTCG	35	1.2115197E-7	45.000004	1
ATACCCG	45	3.8380676E-10	45.000004	1
GCGTAAG	70	0.0	45.000004	1
CGCCCAT	45	3.8380676E-10	45.000004	13
ATTCTCG	35	1.2115197E-7	45.000004	18
CTATGCG	20	7.032434E-4	45.0	1
AGCCGGT	25	3.8900987E-5	45.0	19
GTACCGG	20	7.032434E-4	45.0	14
ACACGGC	20	7.032434E-4	45.0	12
CTCGTTA	20	7.032434E-4	45.0	45
CCCTCGC	20	7.032434E-4	45.0	44
TCCGCGA	20	7.032434E-4	45.0	31
>>END_MODULE