FastQCFastQC Report
Thu 26 May 2016
SRR1547350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547350_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2063772
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296401.4362051621981498No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30650.14851446768344567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC302.1663454E-645.00000413
CGCGATC253.8918894E-545.020
CGTACCC207.0345943E-445.036
TCGCGCC253.8918894E-545.032
CGTTTTT133900.043.3700521
CGGTCTA2350.043.08510631
TAACGCG900.042.51
ATAGCGG4000.042.18752
CGTAAGG2900.041.896552
TACGGGA9050.040.524864
CTCGTCG501.0822987E-940.532
TATAGCG1350.040.01
CGCGTAA451.929584E-840.031
CGACGGT2550.039.70588328
ACGGGAT10350.039.3478285
GGCGATA5150.038.8834958
ACGGGTC1050.038.571435
AGGGCGA10800.038.5416646
CGGGACT7950.038.4905666
TAGGGAT43600.037.8268365
Produced by FastQC (version 0.11.5)