##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547350_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2063772
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32244695634983	31.0	31.0	33.0	30.0	34.0
2	31.736429702505898	31.0	31.0	34.0	30.0	34.0
3	31.797097256867524	31.0	31.0	34.0	30.0	34.0
4	35.60421693869284	37.0	35.0	37.0	33.0	37.0
5	35.4976659243366	37.0	35.0	37.0	33.0	37.0
6	35.53652632170608	37.0	35.0	37.0	33.0	37.0
7	35.93534896296684	37.0	35.0	37.0	35.0	37.0
8	35.92413406132073	37.0	35.0	37.0	35.0	37.0
9	37.63539916231056	39.0	37.0	39.0	35.0	39.0
10	36.99881672975503	39.0	37.0	39.0	33.0	39.0
11	36.732185532122735	39.0	37.0	39.0	32.0	39.0
12	36.5144875499813	39.0	35.0	39.0	32.0	39.0
13	36.425280021242656	39.0	35.0	39.0	32.0	39.0
14	37.52152466454628	40.0	36.0	41.0	32.0	41.0
15	37.638709605518436	40.0	36.0	41.0	32.0	41.0
16	37.66955312893091	40.0	36.0	41.0	32.0	41.0
17	37.5743396072822	40.0	36.0	41.0	32.0	41.0
18	37.41228779148084	39.0	36.0	41.0	32.0	41.0
19	37.35135276571249	39.0	36.0	41.0	32.0	41.0
20	37.16656055029335	39.0	35.0	41.0	32.0	41.0
21	37.05889022624592	39.0	35.0	41.0	31.0	41.0
22	36.994842938076495	39.0	35.0	41.0	31.0	41.0
23	36.91638901971729	39.0	35.0	41.0	31.0	41.0
24	36.77739546810403	38.0	35.0	40.0	31.0	41.0
25	36.70558230269623	38.0	35.0	40.0	31.0	41.0
26	36.58458686327753	38.0	35.0	40.0	31.0	41.0
27	36.500550448402244	38.0	35.0	40.0	30.0	41.0
28	36.5473312943484	38.0	35.0	40.0	30.0	41.0
29	36.53899171032459	38.0	35.0	40.0	30.0	41.0
30	36.46222305564762	38.0	35.0	40.0	30.0	41.0
31	36.350944774907305	38.0	35.0	41.0	30.0	41.0
32	36.217749344404325	38.0	35.0	41.0	30.0	41.0
33	35.99084201161756	38.0	35.0	41.0	29.0	41.0
34	35.71554997354359	38.0	35.0	40.0	27.0	41.0
35	35.52572134906375	38.0	35.0	40.0	26.0	41.0
36	35.382010222059414	38.0	34.0	40.0	25.0	41.0
37	35.31241241765079	38.0	34.0	40.0	25.0	41.0
38	35.225286029658314	38.0	34.0	40.0	25.0	41.0
39	35.18760841798416	38.0	34.0	40.0	24.0	41.0
40	35.04783910238147	38.0	34.0	40.0	24.0	41.0
41	34.96327598203678	38.0	34.0	40.0	24.0	41.0
42	34.89923353936384	38.0	34.0	40.0	24.0	41.0
43	34.85931730830731	38.0	34.0	40.0	23.0	41.0
44	34.73329127442373	38.0	34.0	40.0	23.0	41.0
45	34.65093963868102	38.0	34.0	40.0	23.0	41.0
46	34.587437953417336	38.0	33.0	40.0	23.0	41.0
47	34.461448260757486	38.0	33.0	40.0	23.0	41.0
48	34.398625914102915	37.0	33.0	40.0	23.0	41.0
49	34.29117266829863	37.0	33.0	40.0	23.0	41.0
50	34.23138166425361	37.0	33.0	40.0	23.0	41.0
51	33.79151815219898	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	6.0
12	3.0
13	13.0
14	28.0
15	57.0
16	183.0
17	455.0
18	952.0
19	1860.0
20	3236.0
21	5045.0
22	7690.0
23	11415.0
24	16534.0
25	24498.0
26	34436.0
27	41036.0
28	44013.0
29	46851.0
30	53369.0
31	62811.0
32	75978.0
33	95494.0
34	138518.0
35	175265.0
36	169594.0
37	211098.0
38	316006.0
39	527209.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.728650257877323	23.545769590826893	27.202035883808872	17.523544267486912
2	33.4613998057925	24.086720819935536	29.682687816289782	12.769191557982179
3	27.645350358469834	24.64627875559897	34.571987603281755	13.136383282649438
4	24.97102393093811	27.15808723056617	33.75212959571115	14.11875924278457
5	22.17459099164055	31.145446299300506	32.571427463886515	14.108535245172432
6	20.91955894352671	40.04226242046118	29.41948044648343	9.618698189528688
7	86.36700178120451	3.0972413619333916	9.098582595364217	1.4371742614978786
8	87.06165215925014	2.547907423882095	8.867791597133792	1.522648819733963
9	82.04854993671782	5.801367592931777	9.950372424860886	2.199710045489521
10	34.156631643417974	42.0346821257387	14.878533093771987	8.930153137071343
11	25.880668988628592	24.530277569421425	31.72036445886464	17.86868898308534
12	26.359452497659625	22.603029792050673	33.29103214890017	17.746485561389534
13	24.67840439738498	23.71032265192085	32.647017209265364	18.9642557414288
14	18.432656320562543	29.071961437600663	32.793060473734506	19.70232176810229
15	18.161260061673477	29.007225604378778	35.809333589175544	17.022180744772193
16	23.226257551706293	27.275590520658287	33.84080218163634	15.657349745999074
17	22.46536923652419	27.516847791325787	30.820894943821315	19.19688802832871
18	21.302886171534453	28.469763132749158	31.791302527604792	18.436048168111594
19	23.29612961121674	28.807494238704663	30.245298414747367	17.651077735331228
20	24.544620239057416	28.74581106827692	30.212639768346506	16.49692892431916
21	25.426791331600583	25.77682999866264	32.55369294670148	16.242685723035297
22	23.479580108655412	23.8230773554443	32.91773509864462	19.779607437255667
23	20.593214754343016	26.67809234741047	33.52317988615021	19.205513012096297
24	20.377396340293405	27.58371564300708	33.790845112735326	18.248042903964198
25	21.770282763793674	28.76751889259085	30.588504931746336	18.87369341186914
26	20.55779417493793	31.009094027828652	29.057521858034708	19.375589939198708
27	19.202363439372178	29.975161984947952	32.546085517198605	18.276389058481264
28	18.651333577546357	29.4678385015399	33.219221890790266	18.661606030123483
29	19.766718416569272	28.299153201031896	32.925148708287544	19.008979674111288
30	20.862624359667635	27.534291578720904	32.60815632734624	18.994927734265218
31	21.980577311834836	27.793186456643465	30.497361142606838	19.72887508891486
32	21.852704659235613	29.000587274175636	30.546009927453227	18.600698139135524
33	21.926550025874953	27.896153257239657	30.426471528831673	19.75082518805372
34	20.942042047280417	28.32042493066094	31.14428338014083	19.593249641917808
35	20.50657727694726	28.467098109674904	30.76371808513731	20.262606528240525
36	21.983533064699007	28.878093122689908	29.683172365939647	19.455201446671435
37	20.786695429533882	30.183227604599733	29.711663885351676	19.318413080514706
38	20.53724926978368	30.542472715009218	29.0439544678385	19.876323547368603
39	20.24550192560031	28.826391675049372	29.71830221555482	21.209804183795498
40	21.7616093250611	28.495153534402057	30.053416753401052	19.68982038713579
41	19.29835272501032	28.818348150861627	30.523429913769544	21.35986921035851
42	20.076394097797625	30.290458442114733	28.862248349139342	20.770899110948303
43	20.953768148807136	28.805701405000168	28.97083592567396	21.26969452051874
44	20.43617221282196	29.632730747388763	29.53223515000688	20.3988618897824
45	20.782431392615074	29.13025276047935	28.94021238780253	21.14710345910304
46	20.9628292272596	30.128037399480174	28.207524862242533	20.701608511017692
47	20.57833908009218	29.51483981757675	29.749119573286197	20.157701529044875
48	20.491265508011544	28.79445985312331	29.973611426068388	20.74066321279676
49	20.167344067077178	28.185671673033646	30.53951696214504	21.10746729774413
50	19.09949354870596	29.365501615488533	30.519311241745694	21.015693594059808
51	19.36807941962581	29.05582593426018	30.245637599502274	21.33045704661174
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2353.0
1	3035.0
2	3717.0
3	24069.0
4	44421.0
5	29718.0
6	15015.0
7	14648.0
8	14281.0
9	15028.5
10	15776.0
11	15692.0
12	15608.0
13	15444.0
14	15280.0
15	14489.0
16	13698.0
17	13115.0
18	12532.0
19	12713.0
20	12894.0
21	13578.5
22	14263.0
23	15216.0
24	16169.0
25	18901.5
26	24914.5
27	28195.0
28	33237.0
29	38279.0
30	44966.5
31	51654.0
32	57590.5
33	63527.0
34	70817.0
35	78107.0
36	83554.0
37	89001.0
38	98872.5
39	108744.0
40	114619.0
41	120494.0
42	128156.0
43	135818.0
44	139776.0
45	143734.0
46	146987.0
47	150240.0
48	151361.0
49	152482.0
50	144310.0
51	136138.0
52	126609.0
53	117080.0
54	107793.5
55	98507.0
56	93613.0
57	88719.0
58	83113.0
59	77507.0
60	69251.5
61	60996.0
62	52306.0
63	43616.0
64	35308.0
65	27000.0
66	21025.0
67	15050.0
68	12030.0
69	9010.0
70	7295.5
71	5581.0
72	4421.0
73	3261.0
74	2519.0
75	1294.5
76	812.0
77	587.5
78	363.0
79	284.5
80	206.0
81	164.0
82	122.0
83	81.0
84	40.0
85	30.5
86	21.0
87	21.0
88	21.0
89	22.0
90	23.0
91	12.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2063772.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.238881237752647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70405104441215	17.72528925344457
2	6.436019715464755	2.8625975619211057
3	2.36376253876163	1.577023031213057
4	1.403500981300726	1.2484916656086458
5	0.9253480799804105	1.0289353027133392
6	0.6651929138034887	0.887588772616225
7	0.5347092312801641	0.8323934563818707
8	0.413850060261999	0.7362849872322699
9	0.36143129356295395	0.7234044851838461
>10	4.850197425810174	26.416138225718367
>50	1.803979142314353	28.382148988891164
>100	0.5344386360216431	15.352601535567507
>500	0.0021993356409701907	0.31919164665686117
>1k	0.0010996678204850954	0.4581960382901433
>5k	0.0	0.0
>10k+	2.1993356409701905E-4	1.4497150485610426
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29640	1.4362051621981498	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	3065	0.14851446768344567	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025826496337773747	0.0
2	0.0	0.0	0.0	0.07161643824996172	0.0
3	0.0	0.0	0.0	0.11343307303326143	0.0
4	0.0	0.0	0.0	0.18616397547791133	0.0
5	0.0	0.0	0.0	0.3611833090089409	0.0
6	0.0	0.0	0.0	0.6606834475901407	0.0
7	0.0	0.0	0.0	0.8456845039083775	0.0
8	0.0	0.0	0.0	1.13234407676817	0.0
9	0.0	0.0	0.0	1.2822637384362225	0.0
10	0.0	0.0	0.0	1.4714803767082798	0.0
11	0.0	0.0	0.0	1.6586619064509065	0.0
12	0.0	0.0	0.0	1.817255006851532	0.0
13	0.0	0.0	0.0	1.9015666459279417	0.0
14	0.0	0.0	0.0	1.9553516570628926	0.0
15	4.84549649864423E-5	0.0	0.0	2.0020137883448363	0.0
16	4.84549649864423E-5	0.0	0.0	2.0728064921900287	0.0
17	4.84549649864423E-5	0.0	0.0	2.16036461392053	0.0
18	4.84549649864423E-5	0.0	0.0	2.266529442205825	0.0
19	4.84549649864423E-5	0.0	0.0	2.342894467024458	0.0
20	4.84549649864423E-5	0.0	0.0	2.4240080784117626	0.0
21	4.84549649864423E-5	0.0	0.0	2.5230500268440506	0.0
22	4.84549649864423E-5	0.0	0.0	2.6423461506406714	0.0
23	4.84549649864423E-5	0.0	0.0	2.771575542259513	0.0
24	4.84549649864423E-5	0.0	0.0	2.874542342855703	0.0
25	4.84549649864423E-5	0.0	0.0	2.9652984922753096	0.0
26	4.84549649864423E-5	0.0	0.0	3.0549886324652142	0.0
27	4.84549649864423E-5	0.0	0.0	3.144678772655119	0.0
28	4.84549649864423E-5	0.0	0.0	3.2414917926980307	0.0
29	4.84549649864423E-5	0.0	0.0	3.3581713483853837	0.0
30	4.84549649864423E-5	0.0	0.0	3.489678123358588	0.0
31	4.84549649864423E-5	0.0	0.0	3.6114454503695175	0.0
32	4.84549649864423E-5	0.0	0.0	3.7271559067571416	0.0
33	4.84549649864423E-5	0.0	0.0	3.8415580790901322	0.0
34	4.84549649864423E-5	0.0	0.0	3.9658450642803564	0.0
35	4.84549649864423E-5	0.0	0.0	4.096188920093886	0.0
36	4.84549649864423E-5	0.0	0.0	4.225369856747742	0.0
37	4.84549649864423E-5	0.0	0.0	4.357070451580892	0.0
38	4.84549649864423E-5	0.0	0.0	4.503452900804934	0.0
39	4.84549649864423E-5	0.0	0.0	4.720967238629074	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAC	30	2.1663454E-6	45.000004	13
CGCGATC	25	3.8918894E-5	45.0	20
CGTACCC	20	7.0345943E-4	45.0	36
TCGCGCC	25	3.8918894E-5	45.0	32
CGTTTTT	13390	0.0	43.370052	1
CGGTCTA	235	0.0	43.085106	31
TAACGCG	90	0.0	42.5	1
ATAGCGG	400	0.0	42.1875	2
CGTAAGG	290	0.0	41.89655	2
TACGGGA	905	0.0	40.52486	4
CTCGTCG	50	1.0822987E-9	40.5	32
TATAGCG	135	0.0	40.0	1
CGCGTAA	45	1.929584E-8	40.0	31
CGACGGT	255	0.0	39.705883	28
ACGGGAT	1035	0.0	39.347828	5
GGCGATA	515	0.0	38.883495	8
ACGGGTC	105	0.0	38.57143	5
AGGGCGA	1080	0.0	38.541664	6
CGGGACT	795	0.0	38.490566	6
TAGGGAT	4360	0.0	37.826836	5
>>END_MODULE