FastQCFastQC Report
Thu 26 May 2016
SRR1547349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547349_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1140783
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219441.9235910773565175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG302.1654869E-645.0000049
CCGACCG302.1654869E-645.00000415
CAGGTCG207.0332666E-445.031
CGTTTTT124050.044.0205571
GCGATAT1050.042.8571439
GGCGATA3750.040.88
CGTTAGG1450.040.3448262
ACGGGAC2800.039.3755
CGAGGGT2000.039.3754
TTGCGAG800.039.3751
TACGGGA5100.038.823534
ACGGGAT5450.038.807345
TAGGGAT19100.038.5209435
TAGGGAC13250.038.3773575
GTAGGGA15850.037.760254
ATAGCGG1550.037.7419362
GTTTTTT148450.037.7399752
ATGGGAC9800.037.653065
GGGCGAT12850.037.6459127
TCGTTAG601.5643309E-1037.5000041
Produced by FastQC (version 0.11.5)