##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547349_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1140783
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.327046423377627	31.0	31.0	33.0	30.0	34.0
2	31.735408925273255	31.0	31.0	34.0	30.0	34.0
3	31.696657471228097	31.0	31.0	34.0	30.0	34.0
4	35.55538082176891	37.0	35.0	37.0	33.0	37.0
5	35.48012461616276	37.0	35.0	37.0	33.0	37.0
6	35.528999818545685	37.0	35.0	37.0	33.0	37.0
7	35.92627169233763	37.0	35.0	37.0	35.0	37.0
8	35.915708771957505	37.0	35.0	37.0	35.0	37.0
9	37.62236814538786	39.0	37.0	39.0	35.0	39.0
10	36.97942553491768	39.0	37.0	39.0	32.0	39.0
11	36.7567442712593	39.0	37.0	39.0	32.0	39.0
12	36.55911772878803	39.0	35.0	39.0	32.0	39.0
13	36.4843173504514	39.0	35.0	39.0	32.0	39.0
14	37.62394074946769	40.0	36.0	41.0	32.0	41.0
15	37.711069502262916	40.0	36.0	41.0	32.0	41.0
16	37.73149494689174	40.0	36.0	41.0	32.0	41.0
17	37.632958240085976	40.0	36.0	41.0	32.0	41.0
18	37.40150580785303	39.0	36.0	41.0	32.0	41.0
19	37.245745246904974	39.0	36.0	41.0	32.0	41.0
20	36.977786309929236	39.0	35.0	41.0	32.0	41.0
21	36.87362890225398	38.0	35.0	41.0	31.0	41.0
22	36.800227562998394	38.0	35.0	40.0	31.0	41.0
23	36.700305842566024	38.0	35.0	40.0	31.0	41.0
24	36.56850251099464	38.0	35.0	40.0	31.0	41.0
25	36.471687428722205	38.0	35.0	40.0	31.0	41.0
26	36.35626670453539	38.0	35.0	40.0	30.0	41.0
27	36.26499255336028	38.0	35.0	40.0	30.0	41.0
28	36.269208955603304	38.0	35.0	40.0	30.0	41.0
29	36.23592392242872	38.0	35.0	40.0	30.0	41.0
30	36.13148337589182	38.0	35.0	40.0	30.0	41.0
31	35.950604102620744	38.0	35.0	40.0	30.0	41.0
32	35.68690013788775	38.0	35.0	40.0	27.0	41.0
33	35.283413234594136	38.0	34.0	40.0	25.0	41.0
34	34.83167526164047	38.0	34.0	40.0	23.0	41.0
35	34.52586337629506	38.0	34.0	40.0	21.0	41.0
36	34.32823069768747	38.0	33.0	40.0	20.0	41.0
37	34.22064581958181	38.0	33.0	40.0	18.0	41.0
38	34.11781118757906	38.0	33.0	40.0	18.0	41.0
39	34.05555657824494	38.0	33.0	40.0	18.0	41.0
40	33.91790726194202	38.0	33.0	40.0	18.0	41.0
41	33.81279612336439	38.0	33.0	40.0	18.0	41.0
42	33.72506515261886	38.0	33.0	40.0	17.0	41.0
43	33.678607587946175	38.0	33.0	40.0	17.0	41.0
44	33.54865824613445	37.0	33.0	40.0	16.0	41.0
45	33.46599572398958	37.0	33.0	40.0	17.0	41.0
46	33.37907559982924	37.0	32.0	40.0	17.0	41.0
47	33.27220426671856	37.0	32.0	40.0	15.0	41.0
48	33.20776694603619	37.0	32.0	40.0	15.0	41.0
49	33.0861872941655	36.0	32.0	40.0	15.0	41.0
50	33.023184076200295	36.0	32.0	40.0	15.0	41.0
51	32.613070145680645	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	1.0
13	7.0
14	17.0
15	58.0
16	128.0
17	330.0
18	782.0
19	1420.0
20	2411.0
21	3839.0
22	5791.0
23	8354.0
24	12448.0
25	19292.0
26	27799.0
27	31718.0
28	31387.0
29	30306.0
30	31875.0
31	35644.0
32	42033.0
33	51766.0
34	73163.0
35	92375.0
36	92911.0
37	113547.0
38	164974.0
39	266324.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.77219769228679	22.166091184738903	26.34523831438582	20.716472808588488
2	37.335935055133184	23.619040606320397	26.884604696949378	12.160419641597043
3	26.744700788844156	23.79541069598688	36.79805887710458	12.66182963806438
4	23.995887035483523	25.707693750695793	36.699354741436366	13.597064472384318
5	21.599638143275275	30.04068258380428	35.331171660166746	13.028507612753696
6	19.96321824571369	38.0508825955506	32.66563404258303	9.320265116152678
7	81.89015790031934	3.105323273576132	13.706024721616645	1.2984941044878824
8	82.45178969181694	2.5667458228251996	13.550868131800703	1.430596353557162
9	77.56120138536427	5.729836436903425	14.53089676125959	2.178065416472721
10	31.300080734022156	37.04017328448968	21.73314293778922	9.926603043698933
11	25.71838815971136	23.601947083713554	34.19528516816958	16.484379588405506
12	24.537795531665534	22.11174254875818	36.201363449490394	17.149098470085896
13	22.561170704682663	23.116754018950143	36.870552944775646	17.451522331591548
14	18.652714845855872	27.177210740342378	36.17182233606216	17.998252077739586
15	18.542615028449756	27.408367761441042	38.259160594083184	15.789856616026011
16	22.49411150060967	26.26161154224774	36.19294817682241	15.051328780320183
17	21.730162528719312	26.3784611096063	33.83211355709192	18.059262804582467
18	21.425196553595207	27.2748629669271	34.94845207195409	16.351488407523604
19	22.625337158776034	27.75628669080798	33.37944201482666	16.23893413558933
20	23.533397675105608	27.612613441820223	33.39793808287816	15.456050800196005
21	23.914627058783307	25.40991582097559	35.51210002252839	15.163357097712712
22	22.642693658653748	23.41163919869072	35.84397733837198	18.10168980428355
23	19.85399501921049	26.145813883972675	36.106516313795	17.893674783021837
24	19.409037476890873	26.724977493528566	36.532627151701945	17.333357877878615
25	20.35654458385162	27.628392078072693	34.50288091600243	17.51218242207326
26	19.561301316727196	29.426367679041498	33.0039981311082	18.00833287312311
27	18.49808421058168	28.6041254121073	35.43969361394762	17.45809676336341
28	17.851335442411045	28.246826960079176	36.41840735705213	17.48343024045765
29	18.969777775440203	27.163623581347196	35.712313384754154	18.154285258458444
30	19.442786226653098	26.887585106019284	35.841961179295275	17.827667488032343
31	21.251982191179216	26.90406501499409	33.784339352883066	18.059613440943632
32	21.11996760120023	27.493397079023797	33.62401087673992	17.762624443036053
33	21.04896373806412	27.68177646406021	32.806063905230005	18.463195892645665
34	19.762215951675298	28.958794091426675	32.93772785884783	18.341262098050198
35	19.970756927478757	29.020769068262766	32.26669752266645	18.74177648159203
36	21.699394188026996	29.65393067743822	30.70575210184584	17.940923032688953
37	20.271953561720327	30.43278169467813	30.46679342171123	18.828471321890316
38	20.127842017281115	30.99204669073785	29.72151583605296	19.158595455928076
39	20.482686014781077	29.95381242532541	29.997992606832323	19.565508953061187
40	21.040723783576716	29.26788004379448	30.527628830373526	19.163767342255277
41	19.595313043760296	29.355889770447142	30.837153078192785	20.21164410759978
42	20.169830721530737	29.751144608571483	30.13158506043656	19.94743960946122
43	20.40589665168573	29.447055224350294	30.259830309533015	19.887217814430965
44	20.031066381599306	29.604052655062358	30.66516594304088	19.69971502029746
45	20.43535010602367	29.528052223779632	30.102657560640367	19.933940109556332
46	20.247671993709584	29.954426038957454	30.14403265125795	19.653869316075014
47	20.190693585020114	29.51428974660387	30.78490826037906	19.510108407996963
48	20.24100990284743	29.387447042952076	30.910523736766766	19.461019317433728
49	20.538524855296757	28.791978842601967	31.34732898368927	19.322167318412003
50	19.33207279561494	29.051186772593912	31.780715526090415	19.836024905700732
51	19.51159861253192	29.459502815171685	31.482411641828463	19.546486930467932
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1165.0
1	1488.0
2	1811.0
3	18415.5
4	35020.0
5	24471.5
6	13923.0
7	13804.0
8	13685.0
9	14164.0
10	14643.0
11	14657.0
12	14671.0
13	14417.0
14	14163.0
15	13467.0
16	12771.0
17	12021.5
18	11272.0
19	10667.0
20	10062.0
21	9892.5
22	9723.0
23	9989.0
24	10255.0
25	11610.0
26	14852.0
27	16739.0
28	19728.5
29	22718.0
30	25745.5
31	28773.0
32	31610.5
33	34448.0
34	38424.0
35	42400.0
36	45319.0
37	48238.0
38	52026.5
39	55815.0
40	59462.5
41	63110.0
42	65332.0
43	67554.0
44	69582.5
45	71611.0
46	73830.0
47	76049.0
48	76644.0
49	77239.0
50	73194.0
51	69149.0
52	65032.5
53	60916.0
54	55834.5
55	50753.0
56	47739.0
57	44725.0
58	41024.5
59	37324.0
60	34031.0
61	30738.0
62	26866.0
63	22994.0
64	19655.5
65	16317.0
66	13648.5
67	10980.0
68	8784.5
69	6589.0
70	5267.0
71	3945.0
72	3294.0
73	2643.0
74	2045.5
75	1107.0
76	766.0
77	554.0
78	342.0
79	252.0
80	162.0
81	127.0
82	92.0
83	62.5
84	33.0
85	30.0
86	27.0
87	17.5
88	8.0
89	5.5
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1140783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.92809194781126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92692624376168	23.792030481295566
2	6.398532996406995	3.9578883368795963
3	2.8628995246669073	2.656320592087298
4	1.7720703818483137	2.192270228311906
5	1.2501817228291103	1.933286763756591
6	0.9294962806843319	1.7248527860492155
7	0.8188374493175153	1.772755594596235
8	0.6859021430270165	1.6970915637392285
9	0.60277468633265	1.6778403830438362
>10	7.4616603490079605	50.221539297154
>50	0.26826821007773594	4.981633255216081
>100	0.02131330256969947	1.2649220553907539
>500	8.525321025591227E-4	0.19889651271084832
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.8417736751970763E-4	1.9286721497688268
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21944	1.9235910773565175	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.765909029149277E-5	0.0	0.0	0.019460318044711395	0.0
2	8.765909029149277E-5	0.0	0.0	0.05478693143218298	0.0
3	8.765909029149277E-5	0.0	0.0	0.09616202204976758	0.0
4	8.765909029149277E-5	0.0	0.0	0.145514089883878	0.0
5	8.765909029149277E-5	0.0	0.0	0.2631525890550613	0.0
6	8.765909029149277E-5	0.0	0.0	0.49343301925081284	0.0
7	8.765909029149277E-5	0.0	0.0	0.6392977454958568	0.0
8	1.7531818058298554E-4	0.0	0.0	0.84853999402165	0.0
9	1.7531818058298554E-4	0.0	0.0	0.9753826976734401	0.0
10	1.7531818058298554E-4	0.0	0.0	1.1186176512097392	0.0
11	1.7531818058298554E-4	0.0	0.0	1.2515088320916423	0.0
12	1.7531818058298554E-4	0.0	0.0	1.352316785926859	0.0
13	1.7531818058298554E-4	0.0	0.0	1.4156943082076083	0.0
14	1.7531818058298554E-4	0.0	0.0	1.4533000579426587	0.0
15	1.7531818058298554E-4	0.0	0.0	1.4856462622602196	0.0
16	1.7531818058298554E-4	0.0	0.0	1.5298264437671318	0.0
17	1.7531818058298554E-4	0.0	0.0	1.5886456933527235	0.0
18	1.7531818058298554E-4	0.0	0.0	1.6641201700936987	0.0
19	1.7531818058298554E-4	0.0	0.0	1.7131216015666433	0.0
20	1.7531818058298554E-4	0.0	0.0	1.7691357602629072	0.0
21	1.7531818058298554E-4	0.0	0.0	1.833828168898029	0.0
22	1.7531818058298554E-4	0.0	0.0	1.9056209638467614	0.0
23	1.7531818058298554E-4	0.0	0.0	1.9908255996100923	0.0
24	1.7531818058298554E-4	0.0	0.0	2.060865212752995	0.0
25	1.7531818058298554E-4	0.0	0.0	2.125382303207534	0.0
26	1.7531818058298554E-4	0.0	0.0	2.187357280043619	0.0
27	1.7531818058298554E-4	0.0	0.0	2.250997779595243	0.0
28	1.7531818058298554E-4	0.0	0.0	2.326033960884761	0.0
29	1.7531818058298554E-4	0.0	0.0	2.4060667103208937	0.0
30	1.7531818058298554E-4	0.0	0.0	2.4992483233007503	0.0
31	1.7531818058298554E-4	0.0	0.0	2.5858555045087455	0.0
32	1.7531818058298554E-4	0.0	0.0	2.675092458425485	0.0
33	1.7531818058298554E-4	0.0	0.0	2.764767707793682	0.0
34	1.7531818058298554E-4	0.0	0.0	2.861893979836656	0.0
35	1.7531818058298554E-4	0.0	0.0	2.9614747064077918	0.0
36	1.7531818058298554E-4	0.0	0.0	3.0602665011663044	0.0
37	1.7531818058298554E-4	0.0	0.0	3.16396720498114	0.0
38	1.7531818058298554E-4	0.0	0.0	3.2830959086872786	0.0
39	1.7531818058298554E-4	0.0	0.0	3.445878839358581	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	30	2.1654869E-6	45.000004	9
CCGACCG	30	2.1654869E-6	45.000004	15
CAGGTCG	20	7.0332666E-4	45.0	31
CGTTTTT	12405	0.0	44.020557	1
GCGATAT	105	0.0	42.857143	9
GGCGATA	375	0.0	40.8	8
CGTTAGG	145	0.0	40.344826	2
ACGGGAC	280	0.0	39.375	5
CGAGGGT	200	0.0	39.375	4
TTGCGAG	80	0.0	39.375	1
TACGGGA	510	0.0	38.82353	4
ACGGGAT	545	0.0	38.80734	5
TAGGGAT	1910	0.0	38.520943	5
TAGGGAC	1325	0.0	38.377357	5
GTAGGGA	1585	0.0	37.76025	4
ATAGCGG	155	0.0	37.741936	2
GTTTTTT	14845	0.0	37.739975	2
ATGGGAC	980	0.0	37.65306	5
GGGCGAT	1285	0.0	37.645912	7
TCGTTAG	60	1.5643309E-10	37.500004	1
>>END_MODULE