FastQCFastQC Report
Thu 26 May 2016
SRR1547348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547348_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences699383
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120161.7180857984823765No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT12360.17672720097571717No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10030.14341212182738214No Hit
AACGCGGGGGCACTGGCAAATTTTTGTTTATGTTAAGTTGTGACAGAATGT9190.13140153535330426No Hit
TGCATAGGGGAATTTATATGTGATGAAATCCATAATGTCTGGAACTTTGCT8260.11810410032843234No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTA253.889233E-545.00000411
GGTACCG253.889233E-545.0000041
GATCGGA253.889233E-545.00000410
TTAGCCG253.889233E-545.0000041
ATAATCG253.889233E-545.00000444
CGGCAAC502.1827873E-1145.00000438
TGAACCG253.889233E-545.00000444
ATTCGTG253.889233E-545.00000436
ATTCGCA253.889233E-545.00000426
TTCCGGC253.889233E-545.00000414
CGATTGA253.889233E-545.00000410
ATATGCG253.889233E-545.0000041
CGATTAC253.889233E-545.00000410
CGACAAC253.889233E-545.00000430
TGTGCGC253.889233E-545.00000429
TAGCCGT253.889233E-545.00000444
ATCGTTA253.889233E-545.00000440
ATCGTAG253.889233E-545.0000041
CGCTATC253.889233E-545.00000419
CTCGGCT253.889233E-545.00000445