##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547348_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	699383
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35604954652887	31.0	31.0	33.0	30.0	34.0
2	31.760531783014457	31.0	31.0	34.0	30.0	34.0
3	31.81310240597784	31.0	31.0	34.0	30.0	34.0
4	35.60017186577312	37.0	35.0	37.0	33.0	37.0
5	35.48466862934901	37.0	35.0	37.0	33.0	37.0
6	35.52647261943742	37.0	35.0	37.0	33.0	37.0
7	35.95091959627271	37.0	35.0	37.0	35.0	37.0
8	35.9558696736981	37.0	35.0	37.0	35.0	37.0
9	37.73294460974888	39.0	38.0	39.0	35.0	39.0
10	36.976424934549456	39.0	37.0	39.0	32.0	39.0
11	36.698966088681026	39.0	35.0	39.0	32.0	39.0
12	36.08654628436779	38.0	35.0	39.0	32.0	39.0
13	35.74770762228993	38.0	35.0	39.0	31.0	39.0
14	36.74197828657545	39.0	35.0	41.0	31.0	41.0
15	37.0272540224741	39.0	35.0	41.0	32.0	41.0
16	37.17396762575012	39.0	35.0	41.0	32.0	41.0
17	37.12486720437872	39.0	35.0	41.0	32.0	41.0
18	37.01096537948449	38.0	35.0	40.0	32.0	41.0
19	36.90348778852217	38.0	35.0	40.0	32.0	41.0
20	36.66937429133965	38.0	35.0	40.0	32.0	41.0
21	36.51727451196269	38.0	35.0	40.0	31.0	41.0
22	36.40850721278613	38.0	35.0	40.0	31.0	41.0
23	36.3769994409358	38.0	35.0	40.0	31.0	41.0
24	36.25649894263944	37.0	35.0	40.0	31.0	41.0
25	36.13761844368536	37.0	35.0	40.0	31.0	41.0
26	35.9969801954008	37.0	35.0	40.0	30.0	41.0
27	35.949661344356386	37.0	35.0	40.0	30.0	41.0
28	36.02802470177285	37.0	35.0	40.0	30.0	41.0
29	36.06288828867731	37.0	35.0	40.0	30.0	41.0
30	36.01929271943985	37.0	35.0	40.0	30.0	41.0
31	35.828270060896536	37.0	35.0	40.0	30.0	41.0
32	35.65165152713177	37.0	34.0	40.0	30.0	41.0
33	35.42424680039406	37.0	34.0	40.0	29.0	41.0
34	35.22578758705888	37.0	34.0	40.0	27.0	41.0
35	35.02940305955392	37.0	34.0	40.0	26.0	41.0
36	34.823974846400326	37.0	34.0	40.0	25.0	41.0
37	34.70643552960252	37.0	34.0	40.0	24.0	41.0
38	34.65199325691359	37.0	34.0	40.0	24.0	41.0
39	34.65673886840258	37.0	34.0	40.0	24.0	41.0
40	34.48484449865095	37.0	34.0	40.0	23.0	41.0
41	34.43712386489234	36.0	34.0	40.0	23.0	41.0
42	34.35549763148375	36.0	33.0	40.0	23.0	41.0
43	34.2679947896932	36.0	33.0	40.0	23.0	41.0
44	34.12004581180841	36.0	33.0	40.0	23.0	41.0
45	33.988971707919696	36.0	33.0	40.0	23.0	41.0
46	33.93775370576637	36.0	33.0	40.0	23.0	41.0
47	33.82373463467084	35.0	33.0	40.0	23.0	41.0
48	33.77419811462389	35.0	33.0	40.0	23.0	41.0
49	33.68903161786889	36.0	33.0	40.0	22.0	41.0
50	33.613688065051626	35.0	33.0	40.0	22.0	41.0
51	33.143021777766975	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	9.0
14	8.0
15	29.0
16	57.0
17	132.0
18	310.0
19	624.0
20	1106.0
21	1769.0
22	2879.0
23	4229.0
24	6153.0
25	9182.0
26	12607.0
27	14589.0
28	15670.0
29	17004.0
30	19656.0
31	23545.0
32	28513.0
33	37457.0
34	61051.0
35	91670.0
36	52752.0
37	60840.0
38	88394.0
39	149083.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.13425118997745	24.149714820062826	29.896351498392153	16.819682491567566
2	31.562963354842772	25.246252768511674	30.8560545509399	12.334729325705657
3	27.302350786335953	25.230667602729834	34.84385522667837	12.623126384255837
4	24.500881491257296	27.537558104786648	34.64939811233616	13.3121622916199
5	21.87742624570514	31.04264759080504	34.10563310803951	12.974293055450303
6	19.22151382003852	40.50341515307064	30.39564873610025	9.879422290790597
7	86.6713946435644	3.1426271442113975	9.039539136639009	1.1464390755851943
8	87.81926355087269	2.457594765672028	8.303175799240188	1.4199658842150866
9	85.48949574124622	3.9250310630941843	9.076857744612036	1.5086154510475662
10	42.77756250866835	27.23672151024546	17.092494384335907	12.893221596750278
11	35.22576327991958	27.47607534069315	24.123691882702325	13.174469496684935
12	34.57390299735624	24.314860384081395	27.60476019577256	13.5064764227898
13	21.098167956613185	34.93893903626482	28.246039723584932	15.716853283537061
14	15.013233092597334	37.94473128457512	31.469452360151735	15.572583262675815
15	14.215386991105017	28.573614171348172	42.75182553765247	14.459173299894335
16	15.473781890609295	24.866346479682804	43.54566811032009	16.114203519387804
17	16.562169798236447	25.185770886624354	30.98002096133306	27.27203835380614
18	19.55752427496808	28.24203619476024	33.78477886937487	18.41566066089682
19	23.17785819786869	29.173285596018207	29.69631804032983	17.95253816578327
20	26.648202773015644	28.1635384331618	29.22876306687466	15.959495726947894
21	22.50169077601257	28.143663772210648	32.15734440213731	17.197301049639467
22	21.330515611617667	25.31731540514997	31.27985667366808	22.07231230956429
23	17.954396947023305	28.91076849165622	31.76471261097282	21.370121950347666
24	18.925252687011266	26.471189605695304	38.69467802334343	15.908879683949994
25	17.537458016566028	28.796238970635546	35.5160191197098	18.150283893088623
26	16.425191919163034	35.56234566753839	28.809250439315797	19.203211973982782
27	16.1510931778439	35.63483813589979	32.55054812599105	15.663520560265262
28	14.946316967956042	31.400105521581168	38.4684786447483	15.185098865714494
29	14.80662240860873	28.74033255026216	37.70266077385352	18.750384267275585
30	17.719761561261855	31.833916466371072	33.9043128014264	16.542009170940673
31	25.399816695573097	29.96827203406431	29.003421587313387	15.628489683049201
32	24.916962522680706	29.816566888242924	29.56491650497653	15.701554084099842
33	22.455364228183985	30.641436809301915	28.60449853656723	18.29870042594687
34	19.376221612478428	29.542325163751475	30.227214559118536	20.854238664651557
35	18.41909225703227	28.760922127074867	31.541372895823887	21.278612720068978
36	25.500619832051967	28.372150881562746	29.622395740245334	16.504833546139956
37	19.546228604355555	32.55154900819722	30.89837756994379	17.003844817503428
38	18.943554533067005	32.36181033854126	27.904166958590643	20.790468169801095
39	18.78041073346078	31.306451543717824	30.198903891001066	19.714233831820334
40	21.639645230152862	29.186154081526144	28.532863967239695	20.641336721081295
41	17.722335258363444	27.07786720580855	30.741668013091537	24.458129522736467
42	20.45517263073309	27.795928697151634	28.346985843236112	23.40191282887917
43	20.099430498024688	29.06418943554533	28.62280038262297	22.213579683807012
44	19.356346951527275	31.4813199634535	29.063903469200707	20.098429615818514
45	17.07447850462479	36.11011420065972	26.17535742218498	20.640049872530504
46	21.919749264709036	32.61260282277379	27.2071239935772	18.26052391893998
47	20.504215858835572	29.932097291469766	29.00556633489805	20.558120514796613
48	21.006086793645256	29.09807644738291	31.545376424648587	18.350460334323255
49	20.67179213678342	26.9363138652212	31.456869840988418	20.93502415700696
50	18.837032069695717	31.39796077399651	30.505888761951606	19.259118394356168
51	18.155574270464108	31.7631397960774	29.220898992397586	20.860386941060906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1271.0
1	1345.5
2	1420.0
3	8573.5
4	15727.0
5	10095.0
6	4463.0
7	4715.5
8	4968.0
9	5344.5
10	5721.0
11	5979.0
12	6237.0
13	6156.0
14	6075.0
15	5608.0
16	5141.0
17	5113.0
18	5085.0
19	4768.0
20	4451.0
21	4535.5
22	4620.0
23	4983.0
24	5346.0
25	6128.0
26	8182.0
27	9454.0
28	10193.0
29	10932.0
30	14391.0
31	17850.0
32	19181.5
33	20513.0
34	23418.5
35	26324.0
36	27026.5
37	27729.0
38	30742.5
39	33756.0
40	42762.5
41	51769.0
42	58928.0
43	66087.0
44	63855.5
45	61624.0
46	60058.5
47	58493.0
48	58184.5
49	57876.0
50	52218.0
51	46560.0
52	41841.0
53	37122.0
54	32453.0
55	27784.0
56	24157.0
57	20530.0
58	19022.5
59	17515.0
60	15272.0
61	13029.0
62	10463.0
63	7897.0
64	6166.5
65	4436.0
66	3279.0
67	2122.0
68	1583.5
69	1045.0
70	937.0
71	829.0
72	552.0
73	275.0
74	215.0
75	128.5
76	102.0
77	83.5
78	65.0
79	48.5
80	32.0
81	28.5
82	25.0
83	16.0
84	7.0
85	7.0
86	7.0
87	3.5
88	0.0
89	1.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	699383.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.08704693352616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.62292601557084	18.844323237732176
2	7.3504830532612075	3.394018944694604
3	2.448905209427138	1.6961396851740318
4	1.2418303734972236	1.146807844656349
5	0.7911308248586912	0.9132437242035937
6	0.5355504708853507	0.7418567313961277
7	0.3661389007508594	0.5917146190077337
8	0.3075594123551637	0.5680510870313114
9	0.24385526467529242	0.5066908146491329
>10	2.8983492323041955	16.000433629797133
>50	1.207293864163307	20.463867636709562
>100	0.9816256495951916	32.66921933617543
>500	0.002486702088904855	0.41766217200867467
>1k	0.0012433510444524276	0.32135587736337545
>5k	0.0	0.0
>10k+	6.216755222262138E-4	1.724614659400768
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12016	1.7180857984823765	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1236	0.17672720097571717	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1003	0.14341212182738214	No Hit
AACGCGGGGGCACTGGCAAATTTTTGTTTATGTTAAGTTGTGACAGAATGT	919	0.13140153535330426	No Hit
TGCATAGGGGAATTTATATGTGATGAAATCCATAATGTCTGGAACTTTGCT	826	0.11810410032843234	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.057765201613422114	0.0
2	0.0	0.0	0.0	0.21747740508419564	0.0
3	0.0	0.0	0.0	0.3097015512244364	0.0
4	0.0	0.0	0.0	0.49686652377881646	0.0
5	0.0	0.0	0.0	0.858613949724257	0.0
6	0.0	0.0	0.0	1.2192175102912137	0.0
7	0.0	0.0	0.0	1.3936569805099637	0.0
8	0.0	0.0	0.0	1.7802834784374226	0.0
9	0.0	0.0	0.0	1.8845182110517413	0.0
10	0.0	0.0	0.0	2.1314501496318896	0.0
11	0.0	0.0	0.0	2.5087827413591692	0.0
12	0.0	0.0	0.0	2.755428713594697	0.0
13	0.0	0.0	0.0	2.871817015855404	0.0
14	0.0	0.0	0.0	2.9135681021700557	0.0
15	0.0	0.0	0.0	2.975908765297412	0.0
16	0.0	0.0	0.0	3.1431990769006397	0.0
17	0.0	0.0	0.0	3.3352254773135748	0.0
18	0.0	0.0	0.0	3.576295105828995	0.0
19	0.0	0.0	0.0	3.7074106748376785	0.0
20	0.0	0.0	0.0	3.859544770175998	0.0
21	0.0	0.0	0.0	4.068586168093877	0.0
22	0.0	0.0	0.0	4.287493404901177	0.0
23	0.0	0.0	0.0	4.527419168038113	0.0
24	0.0	0.0	0.0	4.699856873844517	0.0
25	0.0	0.0	0.0	4.840695298570311	0.0
26	0.0	0.0	0.0	4.974813514197514	0.0
27	0.0	0.0	0.0	5.10035273948609	0.0
28	0.0	0.0	0.0	5.23990431566109	0.0
29	0.0	0.0	0.0	5.409625341193595	0.0
30	0.0	0.0	0.0	5.606370186292775	0.0
31	0.0	0.0	0.0	5.791962343951741	0.0
32	0.0	0.0	0.0	5.957822823831863	0.0
33	0.0	0.0	0.0	6.138410570459962	0.0
34	0.0	0.0	0.0	6.329722055011346	0.0
35	0.0	0.0	0.0	6.548772274990957	0.0
36	0.0	0.0	0.0	6.741084641748513	0.0
37	0.0	0.0	0.0	6.951126921872564	0.0
38	0.0	0.0	0.0	7.143725254974742	0.0
39	0.0	0.0	0.0	7.373642196049947	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTA	25	3.889233E-5	45.000004	11
GGTACCG	25	3.889233E-5	45.000004	1
GATCGGA	25	3.889233E-5	45.000004	10
TTAGCCG	25	3.889233E-5	45.000004	1
ATAATCG	25	3.889233E-5	45.000004	44
CGGCAAC	50	2.1827873E-11	45.000004	38
TGAACCG	25	3.889233E-5	45.000004	44
ATTCGTG	25	3.889233E-5	45.000004	36
ATTCGCA	25	3.889233E-5	45.000004	26
TTCCGGC	25	3.889233E-5	45.000004	14
CGATTGA	25	3.889233E-5	45.000004	10
ATATGCG	25	3.889233E-5	45.000004	1
CGATTAC	25	3.889233E-5	45.000004	10
CGACAAC	25	3.889233E-5	45.000004	30
TGTGCGC	25	3.889233E-5	45.000004	29
TAGCCGT	25	3.889233E-5	45.000004	44
ATCGTTA	25	3.889233E-5	45.000004	40
ATCGTAG	25	3.889233E-5	45.000004	1
CGCTATC	25	3.889233E-5	45.000004	19
CTCGGCT	25	3.889233E-5	45.000004	45
>>END_MODULE