FastQCFastQC Report
Thu 26 May 2016
SRR1547347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547347_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2409253
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT682162.831417040883627No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT26220.10883041341029773No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA207.034827E-445.00000440
CGTTTTT324850.043.981841
ACGTTAG1000.042.751
CGGTCTA3150.041.42857431
ATACGCG556.184564E-1140.9090921
CGTTAGG2550.040.5882342
GACCGTA451.9297659E-840.0000049
TAGGGAT34650.039.4155855
ATATGCG1100.038.863641
CATACGA2500.038.69999718
GTTTTTT375150.038.582572
CGACGGT3350.038.2835828
ATAGGGC7350.038.2653054
GGGCGAT25000.038.167
ACGGGAT9100.038.0769235
ACGGTCT3550.038.02816830
TTAGGGA33550.037.6900144
AGTACGG2450.037.653062
GTAGGGA32750.037.6488574
GTACGTC301.1402934E-437.50000444
Produced by FastQC (version 0.11.5)