##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547347_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2409253
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.399007908260362	31.0	31.0	33.0	30.0	34.0
2	31.797962688019897	31.0	31.0	34.0	30.0	34.0
3	31.70226954163801	31.0	31.0	34.0	30.0	34.0
4	35.58549724748708	37.0	35.0	37.0	33.0	37.0
5	35.54382042898774	37.0	35.0	37.0	33.0	37.0
6	35.58977699726845	37.0	35.0	37.0	33.0	37.0
7	35.97330666393276	37.0	35.0	37.0	35.0	37.0
8	35.96890488462606	37.0	35.0	37.0	35.0	37.0
9	37.678348849207616	39.0	37.0	39.0	35.0	39.0
10	37.09283790452891	39.0	37.0	39.0	33.0	39.0
11	36.86561643795815	39.0	37.0	39.0	32.0	39.0
12	36.617482680316265	39.0	35.0	39.0	32.0	39.0
13	36.528775931792964	39.0	35.0	39.0	32.0	39.0
14	37.615734835652376	40.0	36.0	41.0	32.0	41.0
15	37.72197191411612	40.0	36.0	41.0	32.0	41.0
16	37.75872770522648	40.0	36.0	41.0	32.0	41.0
17	37.672671570814686	40.0	36.0	41.0	32.0	41.0
18	37.41889207982723	39.0	36.0	41.0	32.0	41.0
19	37.21121858102906	38.0	36.0	41.0	32.0	41.0
20	36.88319034987193	38.0	35.0	41.0	32.0	41.0
21	36.78609967487848	38.0	35.0	40.0	31.0	41.0
22	36.73348295093957	38.0	35.0	40.0	31.0	41.0
23	36.64275607418565	38.0	35.0	40.0	31.0	41.0
24	36.49833454601904	38.0	35.0	40.0	31.0	41.0
25	36.404252687451255	38.0	35.0	40.0	31.0	41.0
26	36.28220655946055	38.0	35.0	40.0	31.0	41.0
27	36.20336593956716	38.0	35.0	40.0	30.0	41.0
28	36.188787146887435	38.0	35.0	40.0	30.0	41.0
29	36.12060169687451	38.0	35.0	40.0	30.0	41.0
30	35.984134501440906	38.0	35.0	40.0	30.0	41.0
31	35.7696759119943	38.0	35.0	40.0	29.0	41.0
32	35.47642194489329	38.0	34.0	40.0	27.0	41.0
33	35.01222287572123	38.0	34.0	40.0	24.0	41.0
34	34.49630611646016	38.0	34.0	40.0	21.0	41.0
35	34.15116220670888	38.0	33.0	40.0	18.0	41.0
36	33.92398058651374	38.0	33.0	40.0	18.0	41.0
37	33.79709810468224	38.0	33.0	40.0	18.0	41.0
38	33.672094628501036	37.0	33.0	40.0	17.0	41.0
39	33.61190418772956	37.0	33.0	40.0	16.0	41.0
40	33.449018430193924	37.0	33.0	40.0	15.0	41.0
41	33.33414963061165	37.0	33.0	40.0	15.0	41.0
42	33.25703112126456	37.0	33.0	40.0	15.0	41.0
43	33.20310486279357	37.0	32.0	40.0	15.0	41.0
44	33.071692761200254	37.0	32.0	40.0	15.0	41.0
45	33.01016829697836	37.0	32.0	40.0	15.0	41.0
46	32.951340934306195	36.0	32.0	40.0	15.0	41.0
47	32.8306122271094	36.0	31.0	40.0	13.0	41.0
48	32.74799159739554	36.0	31.0	40.0	13.0	41.0
49	32.62126559560163	36.0	31.0	40.0	13.0	41.0
50	32.56368218696833	36.0	31.0	40.0	12.0	41.0
51	32.15691834772023	35.0	31.0	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	8.0
12	7.0
13	13.0
14	49.0
15	91.0
16	262.0
17	637.0
18	1447.0
19	2983.0
20	5159.0
21	8052.0
22	12298.0
23	18591.0
24	28602.0
25	44611.0
26	64600.0
27	72876.0
28	70490.0
29	66291.0
30	68148.0
31	76764.0
32	89457.0
33	111868.0
34	161057.0
35	203990.0
36	202228.0
37	232127.0
38	333066.0
39	533344.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.977839604225874	21.81692831761546	24.75202894839189	23.453203129766777
2	39.50153844365868	22.913450766689923	26.05479789793766	11.530212891713738
3	25.999054478711862	23.01559861085573	38.75122288941842	12.234124021013983
4	22.684085067031152	25.500269170568636	38.579385394560056	13.236260367840154
5	20.951099780720416	28.43669801386571	37.51125348811436	13.100948717299513
6	19.896125479557355	36.336491020245695	34.68626997662761	9.081113523569337
7	78.55961993198721	3.2434534687722705	16.524914568955605	1.6720120302849057
8	78.96136271284087	2.7926913445785893	16.47506509279017	1.7708808497903707
9	73.7413629867847	5.801175717120618	17.71063479011959	2.7468265059750885
10	32.31570117376631	33.45456039693631	24.12320333314932	10.10653509614806
11	26.025369689277134	22.69915197781221	35.05588661713818	16.219591715772484
12	24.988948856761827	20.46999630175826	37.714428497131685	16.826626344348227
13	22.536736490522166	22.19692161844356	37.86044886112002	17.405893029914253
14	18.00827891466774	25.74947504475454	39.022178243629874	17.22006779694785
15	18.015044497194772	26.16445844417336	40.219022244654255	15.601474813977612
16	22.409062061975227	25.23354749376674	37.823300417183255	14.53409002707478
17	21.897907774733497	25.428753227660188	35.51793854775733	17.15540044984898
18	21.617654932877535	25.866731306342672	36.4198778625574	16.095735898222397
19	22.135989869059	27.50939814124959	34.34774181042838	16.006870179263032
20	22.67304430045329	27.426177325502966	34.98457820743608	14.916200166607657
21	23.054282800519495	25.34038558839607	36.85951620689069	14.745815404193749
22	21.78658696284699	22.733519476783883	37.87306688006615	17.60682668030298
23	19.54958653159299	25.29759224124656	37.45999278614575	17.692828441014704
24	19.44731416750337	26.210489309341938	37.2927210218271	17.049475501327592
25	20.327379482354075	27.093377075799012	35.21759649152663	17.361646950320285
26	18.786320905276447	28.303586215312382	34.691707346634	18.218385532777173
27	17.97561318798814	27.51276017919247	36.84009109877626	17.671535534043127
28	17.28411254442767	27.50628514315433	37.38229235368805	17.82730995872995
29	18.80109726956862	26.9189661691819	36.66746497773376	17.61247158351572
30	19.555480474653347	26.230329483869063	36.79248298123941	17.421707060238173
31	20.580320954254287	25.67111050603652	36.32727654588373	17.42129199382547
32	21.52854017407055	26.21297970781815	35.32621937173057	16.932260746380727
33	21.023736402943154	26.475343187286683	34.35222452768556	18.148695882084613
34	19.091436225253222	27.288748836257547	35.14894450686582	18.470870431623414
35	19.211390418523916	29.319253727192617	33.34780531558952	18.121550538693942
36	21.167930474715607	29.463655332171424	31.2728883184954	18.095525874617568
37	20.45036366043749	30.55544602414109	30.79506386419359	18.19912645122783
38	19.994330192802497	30.482850908559623	30.985039761287002	18.53777913735087
39	20.291642264220485	29.546253548298996	30.51422992935985	19.64787425812067
40	21.018921632555816	28.587782188089005	31.13230532451345	19.26099085484173
41	19.160461769685458	28.85794891611632	31.149696607205634	20.831892706992583
42	19.354131757851913	29.42609182182195	31.223246375536316	19.996530044789818
43	19.447438687427184	29.058384486809814	31.60485843537395	19.889318390389054
44	19.77658635269936	28.99064564825695	31.408449008883665	19.82431899016002
45	19.35164135937571	29.364661992742146	30.512486650426503	20.771209997455642
46	19.719307187746576	29.679697399982487	30.64223641103695	19.958759001233993
47	19.461799985306648	29.012602661488852	31.851864457572532	19.673732895631964
48	19.507042224291098	29.041698817019217	32.13435865805708	19.316900300632604
49	20.33775614267161	28.07824665985681	31.89378616525537	19.690211032216208
50	18.802466988730533	29.016296752561892	32.12829868843164	20.052937570275933
51	18.58297986969405	29.50221500191138	32.0791548251678	19.835650303226767
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2632.0
1	3652.5
2	4673.0
3	53119.0
4	101565.0
5	67750.0
6	33935.0
7	33833.0
8	33731.0
9	35279.5
10	36828.0
11	37580.0
12	38332.0
13	37654.5
14	36977.0
15	34068.0
16	31159.0
17	28568.5
18	25978.0
19	24472.5
20	22967.0
21	22677.0
22	22387.0
23	22998.0
24	23609.0
25	25453.0
26	31244.0
27	35191.0
28	38899.5
29	42608.0
30	47571.5
31	52535.0
32	57797.5
33	63060.0
34	69103.5
35	75147.0
36	83274.5
37	91402.0
38	97434.5
39	103467.0
40	111331.5
41	119196.0
42	127470.5
43	135745.0
44	146112.0
45	156479.0
46	158554.5
47	160630.0
48	156315.0
49	152000.0
50	143274.5
51	134549.0
52	126518.0
53	118487.0
54	114763.0
55	111039.0
56	106835.5
57	102632.0
58	96622.0
59	90612.0
60	80983.5
61	71355.0
62	62111.0
63	52867.0
64	45060.5
65	37254.0
66	30171.0
67	23088.0
68	19606.0
69	16124.0
70	12583.0
71	9042.0
72	7428.0
73	5814.0
74	4690.5
75	2607.5
76	1648.0
77	1240.0
78	832.0
79	636.5
80	441.0
81	316.0
82	191.0
83	144.5
84	98.0
85	77.5
86	57.0
87	35.0
88	13.0
89	9.5
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2409253.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.307635093811506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1994996446991	21.62751035909897
2	6.528205444529742	3.5653970419330343
3	2.7207304324366888	2.2289014151282722
4	1.7180342343626194	1.87661807802601
5	1.1840902949020424	1.6167352845654317
6	0.8336050123648856	1.3658268894019514
7	0.6441801667569151	1.231372584992794
8	0.48628552470453235	1.0623446128027225
9	0.42027938747335214	1.0329152535515622
>10	4.856326114518841	31.558288837132743
>50	1.2528395071852674	23.364497826921774
>100	0.15159328570248123	5.459292479068697
>500	0.002938859175343408	0.5608860475755905
>1k	0.001237414389618277	0.56806660545554
>5k	0.0	0.0
>10k+	1.5467679870228462E-4	2.8813466843449413
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68216	2.831417040883627	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT	2622	0.10883041341029773	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.301328254027286E-5	0.0	0.0	0.024696451555731174	0.0
2	8.301328254027286E-5	0.0	0.0	0.07201402260368671	0.0
3	8.301328254027286E-5	0.0	0.0	0.12771593518820978	0.0
4	8.301328254027286E-5	0.0	0.0	0.209317991925298	0.0
5	8.301328254027286E-5	0.0	0.0	0.42714484531097396	0.0
6	8.301328254027286E-5	0.0	0.0	0.8916041611238006	0.0
7	8.301328254027286E-5	0.0	0.0	1.2178048548658027	0.0
8	1.245199238104093E-4	0.0	0.0	1.6171817571670555	0.0
9	1.245199238104093E-4	0.0	0.0	1.9111317906421617	0.0
10	1.245199238104093E-4	0.0	0.0	2.2299027955968094	0.0
11	1.245199238104093E-4	0.0	0.0	2.4835913870398834	0.0
12	1.245199238104093E-4	0.0	0.0	2.6592059862538306	0.0
13	1.245199238104093E-4	0.0	0.0	2.7656705211117303	0.0
14	1.245199238104093E-4	0.0	0.0	2.844948205937691	0.0
15	1.245199238104093E-4	0.0	0.0	2.89641644111266	0.0
16	1.245199238104093E-4	0.0	0.0	2.961083788211533	0.0
17	1.245199238104093E-4	0.0	0.0	3.0323506912723572	0.0
18	1.245199238104093E-4	0.0	0.0	3.1268198068031876	0.0
19	1.245199238104093E-4	0.0	0.0	3.1934379660417567	0.0
20	1.245199238104093E-4	0.0	0.0	3.272300584455016	0.0
21	1.245199238104093E-4	0.0	0.0	3.3640717683032872	0.0
22	1.245199238104093E-4	0.0	0.0	3.46227648154843	0.0
23	1.245199238104093E-4	0.0	0.0	3.572103054349211	0.0
24	1.245199238104093E-4	0.0	0.0	3.6629610920895397	0.0
25	1.245199238104093E-4	0.0	0.0	3.7477591602044287	0.0
26	1.245199238104093E-4	0.0	0.0	3.831561068928834	0.0
27	1.245199238104093E-4	0.0	0.0	3.9273999036215788	0.0
28	1.245199238104093E-4	0.0	0.0	4.025272563736561	0.0
29	1.245199238104093E-4	0.0	0.0	4.143524984715179	0.0
30	1.245199238104093E-4	0.0	0.0	4.285104138087615	0.0
31	1.245199238104093E-4	0.0	0.0	4.406469557161493	0.0
32	1.245199238104093E-4	0.0	0.0	4.530242361429041	0.0
33	1.245199238104093E-4	0.0	0.0	4.655758444629933	0.0
34	1.245199238104093E-4	0.0	0.0	4.781233021189555	0.0
35	1.245199238104093E-4	0.0	0.0	4.926713798841384	0.0
36	1.245199238104093E-4	0.0	0.0	5.053724121128001	0.0
37	1.245199238104093E-4	0.0	0.0	5.19650696709727	0.0
38	1.6602656508054572E-4	0.0	0.0	5.382664253193832	0.0
39	2.0753320635068215E-4	0.0	0.0	5.70450674960247	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGA	20	7.034827E-4	45.000004	40
CGTTTTT	32485	0.0	43.98184	1
ACGTTAG	100	0.0	42.75	1
CGGTCTA	315	0.0	41.428574	31
ATACGCG	55	6.184564E-11	40.909092	1
CGTTAGG	255	0.0	40.588234	2
GACCGTA	45	1.9297659E-8	40.000004	9
TAGGGAT	3465	0.0	39.415585	5
ATATGCG	110	0.0	38.86364	1
CATACGA	250	0.0	38.699997	18
GTTTTTT	37515	0.0	38.58257	2
CGACGGT	335	0.0	38.28358	28
ATAGGGC	735	0.0	38.265305	4
GGGCGAT	2500	0.0	38.16	7
ACGGGAT	910	0.0	38.076923	5
ACGGTCT	355	0.0	38.028168	30
TTAGGGA	3355	0.0	37.690014	4
AGTACGG	245	0.0	37.65306	2
GTAGGGA	3275	0.0	37.648857	4
GTACGTC	30	1.1402934E-4	37.500004	44
>>END_MODULE