FastQCFastQC Report
Thu 26 May 2016
SRR1547346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547346_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2187115
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT512782.34454978361906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAG207.034686E-445.028
CGTTTTT265850.044.0436331
CGACGGT1750.042.4285728
TCGCGAG650.041.5384641
GCGCGAC1500.040.59
TACGGGA12200.039.8360634
CTATGCG1300.039.8076931
CGACGCT356.250879E-638.57142639
CGGTACG356.250879E-638.5714261
GTTTTTT306900.038.548392
GCGTAAG1350.038.3333361
ACGGGAT14050.038.2740175
ATAGGGA39300.038.129774
AGGGCGA12200.037.9918026
AGGGATC34700.037.9322746
TAGGGAT47050.037.6354945
CGAGGGA20050.037.5935174
TAAGGGA36350.037.5722164
TAGGGAC30150.037.537315
CGCGGAT301.1402591E-437.4999964
Produced by FastQC (version 0.11.5)