FastQCFastQC Report
Thu 26 May 2016
SRR1547345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547345_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1864164
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441932.370660521284608No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29590.1587306696191966No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT19960.10707212455556485No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCC207.034416E-445.00000425
CACGATT302.1662308E-645.00000438
CGCGAAA207.034416E-445.00000428
CTACGGT207.034416E-445.00000411
TCTAACG207.034416E-445.00000417
TTGCGTC207.034416E-445.00000416
TACTCGA406.8193913E-945.00000430
TTACGCA207.034416E-445.00000425
CCGCGAA207.034416E-445.00000427
GACGATA207.034416E-445.0000049
GCTCGAT207.034416E-445.00000428
AATCGAC207.034416E-445.00000427
AGTCGAC302.1662308E-645.00000418
AGCGTAA502.1827873E-1145.021
TCCGATC253.8917424E-545.021
AAGCGTA502.1827873E-1145.020
TCGATTG253.8917424E-545.01
CCGCTCG850.045.019
TGCGCGT351.2124656E-745.037
CGTAGAC253.8917424E-545.033