##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547345_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1864164
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.236528009338233	31.0	31.0	33.0	30.0	34.0
2	31.628111582457336	31.0	31.0	34.0	30.0	34.0
3	31.620562353955982	31.0	31.0	34.0	30.0	34.0
4	35.46865243615905	37.0	35.0	37.0	33.0	37.0
5	35.37034563482612	37.0	35.0	37.0	33.0	37.0
6	35.41875929371021	37.0	35.0	37.0	33.0	37.0
7	35.876834334318225	37.0	35.0	37.0	35.0	37.0
8	35.862575395726985	37.0	35.0	37.0	35.0	37.0
9	37.55979302250231	39.0	37.0	39.0	35.0	39.0
10	36.85730922815804	39.0	37.0	39.0	32.0	39.0
11	36.57961960428374	39.0	35.0	39.0	32.0	39.0
12	36.228003008319014	38.0	35.0	39.0	32.0	39.0
13	36.05774974733983	38.0	35.0	39.0	31.0	39.0
14	37.166853345521105	39.0	35.0	41.0	31.0	41.0
15	37.31699195993485	39.0	35.0	41.0	32.0	41.0
16	37.391727873727845	39.0	35.0	41.0	32.0	41.0
17	37.316620211526455	39.0	35.0	41.0	32.0	41.0
18	37.128997234148926	39.0	35.0	40.0	32.0	41.0
19	37.00773268875485	38.0	35.0	40.0	32.0	41.0
20	36.75937202949955	38.0	35.0	40.0	31.0	41.0
21	36.63667359738736	38.0	35.0	40.0	31.0	41.0
22	36.55586150145588	38.0	35.0	40.0	31.0	41.0
23	36.45657731830461	38.0	35.0	40.0	31.0	41.0
24	36.30740160200497	38.0	35.0	40.0	31.0	41.0
25	36.20658911984139	38.0	35.0	40.0	30.0	41.0
26	36.01841093380196	38.0	35.0	40.0	30.0	41.0
27	35.90259172476242	37.0	35.0	40.0	30.0	41.0
28	35.892470834111165	37.0	35.0	40.0	30.0	41.0
29	35.86447758888166	37.0	35.0	40.0	30.0	41.0
30	35.740465967586545	37.0	35.0	40.0	30.0	41.0
31	35.56776549702708	37.0	34.0	40.0	29.0	41.0
32	35.344583952914014	37.0	34.0	40.0	27.0	41.0
33	35.029652434013315	37.0	34.0	40.0	25.0	41.0
34	34.68760205647143	37.0	34.0	40.0	24.0	41.0
35	34.44058730884193	37.0	33.0	40.0	23.0	41.0
36	34.245591053147685	37.0	33.0	40.0	22.0	41.0
37	34.1258773369725	37.0	33.0	40.0	21.0	41.0
38	34.05513087904283	37.0	33.0	40.0	21.0	41.0
39	34.02065108005519	37.0	33.0	40.0	21.0	41.0
40	33.85547087058864	36.0	33.0	40.0	21.0	41.0
41	33.790925583800565	36.0	33.0	40.0	20.0	41.0
42	33.7052898779292	36.0	33.0	40.0	20.0	41.0
43	33.632605822234524	36.0	33.0	40.0	19.0	41.0
44	33.47430537227412	36.0	33.0	40.0	18.0	41.0
45	33.37037674796853	36.0	33.0	40.0	19.0	41.0
46	33.273313399464854	35.0	32.0	40.0	19.0	41.0
47	33.119090916893576	35.0	32.0	40.0	18.0	41.0
48	33.059474381009395	35.0	32.0	40.0	18.0	41.0
49	32.94076433189355	35.0	32.0	40.0	18.0	41.0
50	32.837942369877325	35.0	32.0	40.0	18.0	41.0
51	32.42634821828981	35.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	2.0
12	10.0
13	14.0
14	27.0
15	74.0
16	207.0
17	541.0
18	1181.0
19	2336.0
20	3913.0
21	6509.0
22	9768.0
23	14316.0
24	20798.0
25	30643.0
26	41593.0
27	46748.0
28	47683.0
29	49554.0
30	55009.0
31	63558.0
32	76767.0
33	95498.0
34	142763.0
35	196437.0
36	171000.0
37	175831.0
38	237702.0
39	373540.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.80630244978446	22.52071169703953	28.15878860443609	18.514197248739915
2	34.64684437635315	24.471022935750288	28.28790814542068	12.594224542475876
3	25.94921906012561	24.011621295122104	37.23374123735895	12.80541840739334
4	24.45653923152684	25.97491422428499	36.31767376690034	13.250872777287837
5	21.579968286052083	30.81499267231853	34.3803442186417	13.224694822987676
6	19.374475636263764	39.298151879341084	31.780251093787886	9.547121390607265
7	81.80771648846347	3.874229949725453	12.322950126705589	1.9951034351054948
8	81.90411358657286	3.787810514525546	12.164702247227176	2.1433736516744237
9	77.2404681133205	6.569218158917349	13.540225001662945	2.650088726099206
10	37.37879285298933	31.001939743498962	20.52555461858506	11.093712784926648
11	30.19267618085104	25.760287185033075	28.72563787306267	15.321398761053212
12	28.57334440532056	23.100381726071312	31.903845369827977	16.42242849878015
13	21.362712722700365	30.311496198832295	31.499106301806062	16.82668477666128
14	16.167622591145413	34.39632993663648	32.16170894835433	17.27433852386378
15	16.333434182829407	30.040221783062005	38.302692252398394	15.323651781710193
16	18.299838426232885	27.830384022006648	37.91721114665877	15.952566405101699
17	17.63331981520939	29.227042255938855	31.059499056949925	22.080138871901827
18	18.947796438510775	31.123656502324902	33.185224046811335	16.743323012352988
19	22.786621777912245	32.08719833662704	28.49856557684839	16.627614308612333
20	23.127632547350984	30.547205074231666	30.106042172255233	16.219120206162117
21	20.879171575033098	31.274662529691593	31.59443053293594	16.251735362339364
22	20.233252010016287	28.399754528035086	30.979034033486325	20.387959428462302
23	17.387794206947458	32.46651045723445	31.115341783233664	19.03035355258443
24	17.989297079012363	29.84361890906594	35.36614804276877	16.800935969152928
25	17.898586175894398	32.12995208576069	31.581180625738938	18.390281112605972
26	16.61516905164996	35.10286648599587	29.802849963844384	18.479114498509787
27	17.373042285979132	34.949231934529365	30.924907894369806	16.752817885121697
28	16.342392622108356	32.93014992243172	34.04582429442903	16.681633161030895
29	17.335170081602264	30.405157486143924	33.5319210112415	18.727751421012314
30	18.26459474595583	31.912535592362048	32.18606302878931	17.636806632892814
31	21.958207539679986	31.70933458644197	29.1807480457728	17.15170982810525
32	21.94962460384387	32.03425235118799	29.37134286468358	16.64478018028457
33	21.119976568585166	31.852025894717418	28.37459579736547	18.653401739331947
34	18.127428702624876	32.287127098259596	29.52336811568081	20.062076083434718
35	18.557862934806167	31.532150604775115	30.379462321984548	19.53052413843417
36	21.22179164494111	33.457571329561134	27.638662692767372	17.681974332730384
37	18.07818410826515	35.497413317712386	29.120774781617925	17.30362779240453
38	17.410592630262144	35.25655468081135	28.26897204323225	19.06388064569426
39	18.76299510128937	34.214264410212834	27.965350688029595	19.0573898004682
40	19.910587265927244	32.2143867170485	28.984252458474685	18.89077355854957
41	16.890788578687282	31.86329099800232	29.31614385858755	21.929776564722847
42	18.137460008883338	33.170257552447104	27.953280934510055	20.739001504159503
43	19.21542310655071	32.28787810514525	27.558304956001727	20.938393832302307
44	18.611184423688044	33.76156818820662	27.62965061013945	19.99759677796589
45	17.920472662276495	35.64852663177703	25.756639437302724	20.674361268643747
46	19.854154462804775	34.696142614061856	27.015541551065247	18.43416137206812
47	18.84887810299952	32.74234455766767	28.66362616164672	19.745151177686083
48	18.933849167777083	31.746026637141366	30.06929647820685	19.250827716874696
49	18.903272458860915	31.27224857898769	29.53972933711841	20.28474962503299
50	17.457959707407717	33.01474548376645	29.75569746009471	19.77159734873112
51	17.2356080259033	32.77554979068365	28.365154567945737	21.623687615467308
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3556.0
1	3968.5
2	4381.0
3	31268.0
4	58155.0
5	37922.5
6	17690.0
7	18881.0
8	20072.0
9	23736.0
10	27400.0
11	30506.5
12	33613.0
13	33483.5
14	33354.0
15	30396.0
16	27438.0
17	24777.0
18	22116.0
19	20306.5
20	18497.0
21	17471.5
22	16446.0
23	16562.0
24	16678.0
25	18826.0
26	22049.0
27	23124.0
28	28824.5
29	34525.0
30	36870.0
31	39215.0
32	44653.5
33	50092.0
34	56209.5
35	62327.0
36	66940.5
37	71554.0
38	76287.0
39	81020.0
40	93260.0
41	105500.0
42	115800.5
43	126101.0
44	133728.5
45	141356.0
46	141428.5
47	141501.0
48	144026.0
49	146551.0
50	132760.0
51	118969.0
52	105007.0
53	91045.0
54	83024.5
55	75004.0
56	67525.5
57	60047.0
58	56363.0
59	52679.0
60	48049.5
61	43420.0
62	37957.5
63	32495.0
64	25732.0
65	18969.0
66	15021.5
67	11074.0
68	8320.5
69	5567.0
70	5389.5
71	5212.0
72	4181.5
73	3151.0
74	2265.0
75	1137.5
76	896.0
77	688.0
78	480.0
79	396.5
80	313.0
81	208.0
82	103.0
83	73.5
84	44.0
85	39.0
86	34.0
87	25.5
88	17.0
89	20.0
90	23.0
91	14.5
92	6.0
93	3.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1864164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.724007259877883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.11262646511753	20.350937775366905
2	7.836982702214379	4.031971998546002
3	2.9848595735479764	2.3034764801899246
4	1.8063714435956324	1.858684485163606
5	1.179622088383497	1.5172303582744693
6	0.8900508648306789	1.3737404945138982
7	0.6737738580254251	1.2132514530753375
8	0.5122873239550798	1.054246627245111
9	0.4053319321602456	0.9384085408995441
>10	3.1043674327081314	16.841752274406662
>50	0.73231939716177	13.592824130903661
>100	0.7538495298487112	30.59366872562686
>500	0.004408476596429326	0.8108363403965804
>1k	0.00293898439761955	1.1324707888416041
>5k	0.0	0.0
>10k+	2.09927456972825E-4	2.3864995265498554
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44193	2.370660521284608	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2959	0.1587306696191966	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1996	0.10707212455556485	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.072866979514678E-4	0.0	0.0	0.07837293285354723	0.0
2	1.072866979514678E-4	0.0	0.0	0.23452872172190858	0.0
3	1.072866979514678E-4	0.0	0.0	0.35184672593183863	0.0
4	1.072866979514678E-4	0.0	0.0	0.5399739507897374	0.0
5	1.072866979514678E-4	0.0	0.0	1.0112844148905353	0.0
6	1.072866979514678E-4	0.0	0.0	1.622979523260829	0.0
7	1.072866979514678E-4	0.0	0.0	2.0408075684328204	0.0
8	1.072866979514678E-4	0.0	0.0	2.7449301670883033	0.0
9	1.6093004692720167E-4	0.0	0.0	3.0400758731527913	0.0
10	1.6093004692720167E-4	0.0	0.0	3.4712074688707646	0.0
11	1.6093004692720167E-4	0.0	0.0	3.996214925296272	0.0
12	1.6093004692720167E-4	0.0	0.0	4.366085816483958	0.0
13	1.6093004692720167E-4	0.0	0.0	4.5358133726431795	0.0
14	1.6093004692720167E-4	0.0	0.0	4.613864445402872	0.0
15	1.6093004692720167E-4	0.0	0.0	4.709188676532752	0.0
16	1.6093004692720167E-4	0.0	0.0	4.910887668681511	0.0
17	1.6093004692720167E-4	0.0	0.0	5.14879592138889	0.0
18	1.6093004692720167E-4	0.0	0.0	5.427794979411683	0.0
19	1.6093004692720167E-4	0.0	0.0	5.586847509124734	0.0
20	1.6093004692720167E-4	0.0	0.0	5.748635849635547	0.0
21	1.6093004692720167E-4	0.0	0.0	5.9706120276971335	0.0
22	1.6093004692720167E-4	0.0	0.0	6.2091103572432464	0.0
23	1.6093004692720167E-4	0.0	0.0	6.481403996644072	0.0
24	1.6093004692720167E-4	0.0	0.0	6.670282228387631	0.0
25	1.6093004692720167E-4	0.0	0.0	6.846232413028038	0.0
26	1.6093004692720167E-4	0.0	0.0	7.005016725996211	0.0
27	1.6093004692720167E-4	0.0	0.0	7.175066142249287	0.0
28	1.6093004692720167E-4	0.0	0.0	7.351177256936621	0.0
29	1.6093004692720167E-4	0.0	0.0	7.553305395877187	0.0
30	1.6093004692720167E-4	0.0	0.0	7.767717861733195	0.0
31	1.6093004692720167E-4	0.0	0.0	7.978643509905781	0.0
32	1.6093004692720167E-4	0.0	0.0	8.17116948937969	0.0
33	1.6093004692720167E-4	0.0	0.0	8.358009273862171	0.0
34	1.6093004692720167E-4	0.0	0.0	8.541952317499963	0.0
35	1.6093004692720167E-4	0.0	0.0	8.761729118253545	0.0
36	1.6093004692720167E-4	0.0	0.0	8.956132614941604	0.0
37	1.6093004692720167E-4	0.0	0.0	9.17446104527284	0.0
38	1.6093004692720167E-4	0.0	0.0	9.384635686559767	0.0
39	1.6093004692720167E-4	0.0	0.0	9.621578358985582	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCCC	20	7.034416E-4	45.000004	25
CACGATT	30	2.1662308E-6	45.000004	38
CGCGAAA	20	7.034416E-4	45.000004	28
CTACGGT	20	7.034416E-4	45.000004	11
TCTAACG	20	7.034416E-4	45.000004	17
TTGCGTC	20	7.034416E-4	45.000004	16
TACTCGA	40	6.8193913E-9	45.000004	30
TTACGCA	20	7.034416E-4	45.000004	25
CCGCGAA	20	7.034416E-4	45.000004	27
GACGATA	20	7.034416E-4	45.000004	9
GCTCGAT	20	7.034416E-4	45.000004	28
AATCGAC	20	7.034416E-4	45.000004	27
AGTCGAC	30	2.1662308E-6	45.000004	18
AGCGTAA	50	2.1827873E-11	45.0	21
TCCGATC	25	3.8917424E-5	45.0	21
AAGCGTA	50	2.1827873E-11	45.0	20
TCGATTG	25	3.8917424E-5	45.0	1
CCGCTCG	85	0.0	45.0	19
TGCGCGT	35	1.2124656E-7	45.0	37
CGTAGAC	25	3.8917424E-5	45.0	33
>>END_MODULE