FastQCFastQC Report
Thu 26 May 2016
SRR1547344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547344_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4449055
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT913682.0536495952511262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT443250.043.55331
CGGTCTA5800.040.73275831
ATTCGCG800.039.3751
CACGACG6200.038.83064726
TACGGGA18800.038.7765964
TAGGGAT83700.038.3602145
ACGGGAT26350.038.168885
ACGGTCT6150.038.0487830
GTAGGGA74450.038.0188034
GTTTTTT519950.037.9031642
TAGGGAC58050.037.829465
ATAGGGA69050.037.6031844
TAAGGGA67450.037.3276524
AGGGATC58550.037.3142626
CGTTAGG6650.037.218052
CGACGGT6400.036.91406228
AGGGCGA26850.036.787716
TCACGAC6600.036.47727225
TTAGGGA70850.036.425554
GGGCGAT50500.036.400997
Produced by FastQC (version 0.11.5)