##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547344_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4449055
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.326123637491555	31.0	31.0	33.0	30.0	34.0
2	31.73666879820546	31.0	31.0	34.0	30.0	34.0
3	31.72573928620797	31.0	31.0	34.0	30.0	34.0
4	35.573741614792354	37.0	35.0	37.0	33.0	37.0
5	35.490439655162724	37.0	35.0	37.0	33.0	37.0
6	35.538724515655574	37.0	35.0	37.0	33.0	37.0
7	35.9393415006108	37.0	35.0	37.0	35.0	37.0
8	35.929390173868384	37.0	35.0	37.0	35.0	37.0
9	37.63248420170126	39.0	37.0	39.0	35.0	39.0
10	37.00508647341964	39.0	37.0	39.0	32.0	39.0
11	36.74691816576779	39.0	37.0	39.0	32.0	39.0
12	36.56823617599692	39.0	35.0	39.0	32.0	39.0
13	36.49572077665931	39.0	35.0	39.0	32.0	39.0
14	37.619013251128614	40.0	36.0	41.0	32.0	41.0
15	37.7116929325441	40.0	36.0	41.0	32.0	41.0
16	37.730949606152315	40.0	36.0	41.0	32.0	41.0
17	37.63648392748573	40.0	36.0	41.0	32.0	41.0
18	37.4272060021735	39.0	36.0	41.0	32.0	41.0
19	37.282758023894964	39.0	36.0	41.0	32.0	41.0
20	37.028981210616635	39.0	35.0	41.0	32.0	41.0
21	36.91751866407586	39.0	35.0	41.0	31.0	41.0
22	36.85983045837824	38.0	35.0	41.0	31.0	41.0
23	36.7616687139179	38.0	35.0	40.0	31.0	41.0
24	36.62799246132044	38.0	35.0	40.0	31.0	41.0
25	36.54412229113823	38.0	35.0	40.0	31.0	41.0
26	36.423829554815576	38.0	35.0	40.0	30.0	41.0
27	36.34836431556814	38.0	35.0	40.0	30.0	41.0
28	36.371014743580375	38.0	35.0	40.0	30.0	41.0
29	36.34516700737572	38.0	35.0	40.0	30.0	41.0
30	36.24216648254517	38.0	35.0	40.0	30.0	41.0
31	36.08266924099612	38.0	35.0	40.0	30.0	41.0
32	35.85742792570557	38.0	35.0	40.0	29.0	41.0
33	35.50370674221829	38.0	35.0	40.0	26.0	41.0
34	35.11055583713845	38.0	34.0	40.0	24.0	41.0
35	34.84484008401784	38.0	34.0	40.0	23.0	41.0
36	34.67500087097147	38.0	34.0	40.0	22.0	41.0
37	34.57757276545244	38.0	34.0	40.0	22.0	41.0
38	34.47010477505897	38.0	33.0	40.0	21.0	41.0
39	34.415506439007835	38.0	33.0	40.0	21.0	41.0
40	34.27416743555654	38.0	33.0	40.0	20.0	41.0
41	34.15973369625684	38.0	33.0	40.0	19.0	41.0
42	34.075860379338984	38.0	33.0	40.0	18.0	41.0
43	34.03989139266653	38.0	33.0	40.0	18.0	41.0
44	33.904501517738034	38.0	33.0	40.0	18.0	41.0
45	33.8246110690922	37.0	33.0	40.0	18.0	41.0
46	33.7663843220639	37.0	33.0	40.0	19.0	41.0
47	33.64124763573388	37.0	33.0	40.0	18.0	41.0
48	33.578454076202696	37.0	33.0	40.0	18.0	41.0
49	33.46986404978136	37.0	32.0	40.0	18.0	41.0
50	33.41085736184426	37.0	32.0	40.0	18.0	41.0
51	32.99093874991431	36.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	1.0
11	11.0
12	16.0
13	20.0
14	53.0
15	193.0
16	471.0
17	1126.0
18	2467.0
19	4698.0
20	8239.0
21	12993.0
22	19689.0
23	29350.0
24	43415.0
25	66244.0
26	96037.0
27	115005.0
28	116051.0
29	115519.0
30	122604.0
31	139902.0
32	165864.0
33	203741.0
34	285971.0
35	362164.0
36	366392.0
37	448167.0
38	657990.0
39	1064343.0
40	317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.032377662222654	22.23926204553551	27.2984262950222	20.429933997219635
2	36.15911693606845	23.649539059418238	27.865153386505675	12.326190618007644
3	26.488906071064527	24.30172250062092	36.79050944526422	12.418861983050334
4	24.43635783329269	25.577454088564878	36.46922773487853	13.516960343263907
5	21.41423291013485	29.767377566696744	35.019189468325294	13.799200054843109
6	20.13276976796196	38.27592601125407	31.870273574950186	9.721030645833778
7	83.14790444262883	2.854358959374519	12.570871791874904	1.4268648061217495
8	83.94054467746521	2.294801929848024	12.343542617477194	1.4211107752095669
9	78.95274389729954	5.340077836754097	13.496641421605263	2.2105368443411018
10	32.80721411625615	38.62301095401158	19.16941912383641	9.40035580589586
11	24.343281887951488	23.769969128275555	34.21922183474918	17.667527149023783
12	24.87818199595195	21.80186578947664	35.37526508438309	17.94468713018832
13	22.95525679048697	22.596259205606582	35.70809980995964	18.740384193946806
14	18.486240336430996	26.291313548607516	35.843386966445685	19.379059148515807
15	18.48459953855369	27.112386787756055	37.130581662847504	17.272432010842753
16	22.890411559308664	26.213162120944784	35.025820089884256	15.870606229862297
17	22.355488974624947	25.417487533869554	33.38969736269837	18.837326128807128
18	21.601845785228548	26.63484717541141	34.73800616085888	17.025300878501167
19	22.865574824316624	27.244572161953496	32.70773231618849	17.18212069754139
20	23.730185398921794	27.393952198837727	32.447766997710744	16.428095404529728
21	24.37805331694034	24.924011953100152	34.887678394625375	15.810256335334133
22	23.117178816625103	22.270189961688494	35.96885181235116	18.64377940933524
23	20.677155036294224	25.181707126569574	35.47436478083548	18.66677305630072
24	20.074937261957874	25.878956317689937	36.10791505162332	17.938191368728866
25	20.999380767376444	27.12106278749083	33.3575332289666	18.52202321616613
26	20.147064938509413	28.80195906771213	32.20362526424151	18.847350729536945
27	19.050764712955896	27.611009528989864	35.02211143714789	18.316114320906348
28	18.252842457555595	27.333939454558326	35.89186917221747	18.521348915668607
29	19.410908608682067	26.551413727184762	35.154161951245825	18.883515712887345
30	20.469335622958134	25.400764881531025	35.20527392895795	18.924625566552898
31	21.929668210440195	26.01916586780788	33.172482695763485	18.87868322598844
32	21.835940441284723	26.626036315577128	32.875857906903825	18.662165336234324
33	21.415019594048623	26.2183542347757	32.954638681697574	19.411987489478104
34	20.107348639205405	27.18183973900075	33.10496273927834	19.6058488825155
35	19.840168305404184	28.03166065602695	32.15383941084118	19.974331627727686
36	21.81670040042211	28.255595851253805	31.150862374144623	18.776841374179458
37	20.856878595566926	29.273969415977103	30.531607273904232	19.33754471455174
38	20.634606674900624	29.52813575017616	30.332104233370906	19.505153341552305
39	20.593856448167084	28.533947096630634	30.15094216637016	20.72125428883212
40	21.495148969837416	27.714042645011133	31.130138872187462	19.660669512963988
41	19.770175913761463	27.800173295227864	31.552318413685605	20.87733237732507
42	20.444184214400586	28.517876268106374	30.243950681661612	20.793988835831428
43	20.47495479377081	28.02302960965868	30.47251607363811	21.029499522932397
44	20.505140979376517	28.522349128073266	30.495981730951854	20.476528161598363
45	20.725187708401	28.1497531498262	30.02230810812633	21.10275103364647
46	20.66247776213151	28.358786304057826	30.48350717174771	20.495228762062958
47	20.66973773082149	28.266294752481148	30.887615459912272	20.176352056785092
48	20.091075520531888	28.582856359384184	31.312110099785233	20.01395802029869
49	20.64433907874818	27.93599989211192	31.274394225290543	20.145266803849356
50	19.783864213861147	27.98335826372117	31.863620476707975	20.369157045709706
51	19.552736479994064	28.366967816761086	31.6532387214813	20.427056981763542
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4406.0
1	5815.0
2	7224.0
3	74469.0
4	141714.0
5	95638.5
6	49563.0
7	48799.5
8	48036.0
9	48866.5
10	49697.0
11	49427.5
12	49158.0
13	47377.5
14	45597.0
15	42314.0
16	39031.0
17	36987.0
18	34943.0
19	32987.5
20	31032.0
21	31115.0
22	31198.0
23	33902.5
24	36607.0
25	40646.0
26	50955.5
27	57226.0
28	64512.5
29	71799.0
30	85823.0
31	99847.0
32	113414.5
33	126982.0
34	141614.0
35	156246.0
36	167610.0
37	178974.0
38	191355.5
39	203737.0
40	221484.5
41	239232.0
42	254159.0
43	269086.0
44	275329.0
45	281572.0
46	295055.0
47	308538.0
48	310102.0
49	311666.0
50	299111.5
51	286557.0
52	269305.5
53	252054.0
54	236411.5
55	220769.0
56	207619.5
57	194470.0
58	179978.5
59	165487.0
60	150178.5
61	134870.0
62	117049.5
63	99229.0
64	83866.5
65	68504.0
66	55805.5
67	43107.0
68	34990.0
69	26873.0
70	21833.0
71	16793.0
72	13512.5
73	10232.0
74	8614.0
75	4920.0
76	2844.0
77	2103.0
78	1362.0
79	995.5
80	629.0
81	461.0
82	293.0
83	190.0
84	87.0
85	63.5
86	40.0
87	34.0
88	28.0
89	21.5
90	15.0
91	11.5
92	8.0
93	8.0
94	8.0
95	5.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4449055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.8660221911002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.29341006752709	17.77563508526756
2	6.105010287074763	2.6698458082814356
3	2.120477307187081	1.3909921156403133
4	1.1999273666303318	1.0495055370578905
5	0.8378919617208017	0.9160682114365767
6	0.6510098247093248	0.8540997164231009
7	0.5048315483541582	0.7727060487355649
8	0.3991344693475794	0.6981986531189741
9	0.32530316390084113	0.6401777580621808
>10	4.179912550810963	22.410356186778785
>50	1.6366234352197757	25.590586811315237
>100	0.7406244114141316	21.788025357101994
>500	0.003860954032440979	0.6318087660615264
>1k	0.0018783019617226646	0.7272303716819265
>5k	0.0	0.0
>10k+	1.0435010898459248E-4	2.0847635730369687
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	91368	2.0536495952511262	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4953366501425584E-5	0.0	0.0	0.020656071907405055	0.0
2	4.4953366501425584E-5	0.0	0.0	0.06783463005065121	0.0
3	4.4953366501425584E-5	0.0	0.0	0.10930411064821631	0.0
4	4.4953366501425584E-5	0.0	0.0	0.17682406713335752	0.0
5	4.4953366501425584E-5	0.0	0.0	0.3398699274340281	0.0
6	4.4953366501425584E-5	0.0	0.0	0.643057907802893	0.0
7	4.4953366501425584E-5	0.0	0.0	0.8446288031952853	0.0
8	4.4953366501425584E-5	0.0	0.0	1.1263965044262207	0.0
9	4.4953366501425584E-5	0.0	0.0	1.290566198889427	0.0
10	4.4953366501425584E-5	0.0	0.0	1.4863381100031354	0.0
11	4.4953366501425584E-5	0.0	0.0	1.6673203635378748	0.0
12	6.743004975213837E-5	0.0	0.0	1.800899292096861	0.0
13	6.743004975213837E-5	0.0	0.0	1.8758140773714866	0.0
14	6.743004975213837E-5	0.0	0.0	1.926588904834847	0.0
15	6.743004975213837E-5	0.0	0.0	1.9669345512698764	0.0
16	2.2476683250712793E-4	0.0	0.0	2.0282059898113194	0.0
17	2.2476683250712793E-4	0.0	0.0	2.103188205135697	0.0
18	2.2476683250712793E-4	0.0	0.0	2.197972378403953	0.0
19	2.2476683250712793E-4	0.0	0.0	2.263267143247274	0.0
20	2.2476683250712793E-4	0.0	0.0	2.335799400097324	0.0
21	2.2476683250712793E-4	0.0	0.0	2.4193227550569727	0.0
22	2.2476683250712793E-4	0.0	0.0	2.517208710613827	0.0
23	2.2476683250712793E-4	0.0	0.0	2.6260857642802797	0.0
24	2.2476683250712793E-4	0.0	0.0	2.7140595025235696	0.0
25	2.472435157578407E-4	0.0	0.0	2.7964590233206827	0.0
26	2.472435157578407E-4	0.0	0.0	2.875959051978454	0.0
27	2.472435157578407E-4	0.0	0.0	2.962651619276453	0.0
28	2.472435157578407E-4	0.0	0.0	3.0547385905546234	0.0
29	2.472435157578407E-4	0.0	0.0	3.165121581998874	0.0
30	2.472435157578407E-4	0.0	0.0	3.2906089045876032	0.0
31	2.472435157578407E-4	0.0	0.0	3.3984295541412726	0.0
32	2.472435157578407E-4	0.0	0.0	3.5069020275092124	0.0
33	2.472435157578407E-4	0.0	0.0	3.617374925686466	0.0
34	2.472435157578407E-4	0.0	0.0	3.733826621608409	0.0
35	2.472435157578407E-4	0.0	0.0	3.8612019855901982	2.2476683250712792E-5
36	2.697201990085535E-4	0.0	0.0	3.9814522409815116	2.2476683250712792E-5
37	2.697201990085535E-4	0.0	0.0	4.1057033459914525	2.2476683250712792E-5
38	2.697201990085535E-4	0.0	0.0	4.258477362046547	2.2476683250712792E-5
39	2.697201990085535E-4	0.0	0.0	4.485739106394504	2.2476683250712792E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	44325	0.0	43.5533	1
CGGTCTA	580	0.0	40.732758	31
ATTCGCG	80	0.0	39.375	1
CACGACG	620	0.0	38.830647	26
TACGGGA	1880	0.0	38.776596	4
TAGGGAT	8370	0.0	38.360214	5
ACGGGAT	2635	0.0	38.16888	5
ACGGTCT	615	0.0	38.04878	30
GTAGGGA	7445	0.0	38.018803	4
GTTTTTT	51995	0.0	37.903164	2
TAGGGAC	5805	0.0	37.82946	5
ATAGGGA	6905	0.0	37.603184	4
TAAGGGA	6745	0.0	37.327652	4
AGGGATC	5855	0.0	37.314262	6
CGTTAGG	665	0.0	37.21805	2
CGACGGT	640	0.0	36.914062	28
AGGGCGA	2685	0.0	36.78771	6
TCACGAC	660	0.0	36.477272	25
TTAGGGA	7085	0.0	36.42555	4
GGGCGAT	5050	0.0	36.40099	7
>>END_MODULE