FastQCFastQC Report
Thu 26 May 2016
SRR1547343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547343_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences710881
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102601.4432795362374293No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11100.1561442773122365No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG253.889299E-545.01
TCTGCGA207.031469E-445.035
GCCCCCG253.889299E-545.031
CGGGTCA453.8380676E-1045.06
TTGTGCG253.889299E-545.01
TGGAACG207.031469E-445.01
TGAGTCG207.031469E-445.01
GTTACGC207.031469E-445.013
TCGTCTA207.031469E-445.020
ACTCGTC207.031469E-445.018
ACTCGCG253.889299E-545.01
CGAGACT406.8084773E-945.016
ACCGTAA207.031469E-445.040
CCGCGGA207.031469E-445.014
TTGCACG351.2110468E-745.01
GTAAGCG253.889299E-545.01
GATTCGC207.031469E-445.011
AATACGG207.031469E-445.02
CACCCGC207.031469E-445.018
GCGTAAG207.031469E-445.01