FastQCFastQC Report
Thu 26 May 2016
SRR1547342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547342_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2690586
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469831.7461995267945347No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT46710.17360530382600667No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35180.13075218558336363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAT302.166591E-645.00000411
CGGCGAA253.892206E-545.038
CGTATAG207.034977E-445.01
CGACGGC551.8189894E-1245.028
ATCGTCC207.034977E-445.033
CCCCGTA207.034977E-445.041
CGCTAGC207.034977E-445.030
TCGCTAA253.892206E-545.041
ACTACCG351.2127384E-745.042
GACGGCG253.892206E-545.09
CGTCGTT253.892206E-545.045
CGTCGCA253.892206E-545.037
CCGCGAT351.2127384E-745.010
CGGTACC207.034977E-445.033
AATCGGC253.892206E-545.010
CGACGGT7300.044.3835628
CGTTTTT168350.043.449661
CGAACGG1450.043.4482732
GACGGTC7500.043.229
GCGCGAC2250.043.09