FastQCFastQC Report
Thu 26 May 2016
SRR1547341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547341_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1069560
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171611.6044915666255284No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18620.17409028011518757No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16680.15595198025356222No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT207.0330664E-445.018
TATCACG351.211829E-745.01
AACCGTA207.0330664E-445.027
CTATGCG351.211829E-745.01
TCGTTGT207.0330664E-445.040
TCGTTGC207.0330664E-445.043
GTCGCAT253.8906233E-545.021
TCCGCCG406.8139343E-945.016
CTCCGTC207.0330664E-445.016
GGTCGCT502.1827873E-1145.08
CGAGTCT207.0330664E-445.034
TAGACCG253.8906233E-545.025
ACGCACG207.0330664E-445.032
GTTTACG253.8906233E-545.01
ACACCGC207.0330664E-445.026
CGTGATA253.8906233E-545.010
CGGTTCC253.8906233E-545.039
CGCATGA253.8906233E-545.018
CGTTGCT207.0330664E-445.044
GGCCGTA351.211829E-745.09