FastQCFastQC Report
Thu 26 May 2016
SRR1547340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547340_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2370573
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263771.112684570354931No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTA253.892064E-545.00000444
GCGTAAG700.045.01
CGGTCGA302.1664837E-644.99999619
CGATCAT302.1664837E-644.99999610
CGACGGT5950.044.243728
TAGACGG2600.043.269232
CGTTTTT136300.043.151141
CGGTCTA6050.043.14049531
ATGTCGC650.041.5384633
ATCGATC650.041.5384641
GGCGATA5600.040.982148
TTGCGTC900.040.00000416
TAGGGAC29900.039.6571925
ACGTAGG2900.039.5689662
CGTTAGG3250.039.461542
TACGGGT2400.039.3749964
ACGGGTA2350.039.2553185
ATAGGGA34000.039.1764684
TATAGCG1500.039.01
GACGGTC6800.038.71323829
Produced by FastQC (version 0.11.5)