##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547340_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2370573
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39200944244282	31.0	31.0	33.0	30.0	34.0
2	31.818320296400913	31.0	31.0	34.0	30.0	34.0
3	31.887496820388993	31.0	31.0	34.0	30.0	34.0
4	35.68040427356593	37.0	35.0	37.0	33.0	37.0
5	35.576181792334594	37.0	35.0	37.0	33.0	37.0
6	35.61770972672008	37.0	35.0	37.0	33.0	37.0
7	35.97833055552392	37.0	35.0	37.0	35.0	37.0
8	35.97015573871802	37.0	35.0	37.0	35.0	37.0
9	37.70160674233613	39.0	37.0	39.0	35.0	39.0
10	37.051272413884746	39.0	37.0	39.0	33.0	39.0
11	36.80844167211894	39.0	37.0	39.0	32.0	39.0
12	36.43775956277238	39.0	35.0	39.0	32.0	39.0
13	36.250002847412844	39.0	35.0	39.0	32.0	39.0
14	37.28271139509309	39.0	36.0	41.0	32.0	41.0
15	37.45283608646517	39.0	36.0	41.0	32.0	41.0
16	37.53112222234877	39.0	35.0	41.0	32.0	41.0
17	37.478809131800624	39.0	35.0	41.0	32.0	41.0
18	37.32130797068894	39.0	36.0	41.0	32.0	41.0
19	37.236934698910346	39.0	35.0	41.0	32.0	41.0
20	37.05692885222265	39.0	35.0	41.0	32.0	41.0
21	36.9569361500363	39.0	35.0	41.0	31.0	41.0
22	36.907821442326394	39.0	35.0	41.0	31.0	41.0
23	36.832321552637275	38.0	35.0	40.0	31.0	41.0
24	36.69869099158726	38.0	35.0	40.0	31.0	41.0
25	36.6182939736511	38.0	35.0	40.0	31.0	41.0
26	36.51564495166358	38.0	35.0	40.0	31.0	41.0
27	36.42187310831601	38.0	35.0	40.0	31.0	41.0
28	36.495229212515284	38.0	35.0	40.0	31.0	41.0
29	36.49966822367419	38.0	35.0	40.0	31.0	41.0
30	36.43169436250223	38.0	35.0	40.0	30.0	41.0
31	36.29052469592795	38.0	35.0	40.0	30.0	41.0
32	36.13995013020059	38.0	35.0	40.0	30.0	41.0
33	35.91453121249588	38.0	35.0	40.0	29.0	41.0
34	35.6568610205212	38.0	35.0	40.0	27.0	41.0
35	35.46826822038385	38.0	34.0	40.0	27.0	41.0
36	35.31047303753143	38.0	34.0	40.0	26.0	41.0
37	35.22180249247756	38.0	34.0	40.0	25.0	41.0
38	35.12330352197549	38.0	34.0	40.0	25.0	41.0
39	35.0755876321885	38.0	34.0	40.0	25.0	41.0
40	34.933601707266554	38.0	34.0	40.0	24.0	41.0
41	34.837027587844794	38.0	34.0	40.0	24.0	41.0
42	34.76445441671697	38.0	34.0	40.0	23.0	41.0
43	34.73759255673628	38.0	34.0	40.0	23.0	41.0
44	34.61917688255118	38.0	34.0	40.0	23.0	41.0
45	34.527769024619786	37.0	33.0	40.0	23.0	41.0
46	34.47450721829701	37.0	33.0	40.0	23.0	41.0
47	34.341947706314045	37.0	33.0	40.0	23.0	41.0
48	34.27677401202157	37.0	33.0	40.0	23.0	41.0
49	34.17813203811905	37.0	33.0	40.0	23.0	41.0
50	34.10504295796839	37.0	33.0	40.0	23.0	41.0
51	33.68338794038404	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	5.0
12	13.0
13	13.0
14	31.0
15	79.0
16	232.0
17	528.0
18	1084.0
19	2027.0
20	3619.0
21	5655.0
22	8598.0
23	13115.0
24	19235.0
25	28802.0
26	39155.0
27	45859.0
28	48209.0
29	51798.0
30	59690.0
31	71652.0
32	89486.0
33	115013.0
34	177639.0
35	229180.0
36	192008.0
37	239998.0
38	355443.0
39	572228.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.312176423168577	23.145922947743014	29.507971279517655	16.03392934957076
2	33.13582834192408	24.889552019701565	29.25208377890071	12.722535859473638
3	27.557008368862718	24.86424168333985	34.28027738441297	13.298472563384465
4	25.420605060464286	27.36068452648368	33.239811640476795	13.978898772575238
5	22.220998889298073	31.00220917052544	32.85876452655118	13.918027413625314
6	21.44333880458438	39.62691720524953	29.115492330335325	9.814251659830767
7	86.47483119060244	3.3883369126367335	8.637616306268567	1.4992155904922566
8	87.18786554980589	2.8874875399323288	8.431294881026655	1.4933520292351259
9	82.39299106165473	5.2958082286434545	9.708412269944862	2.60278843975697
10	37.84768492680883	32.16779234387636	18.185350124210476	11.799172605104335
11	31.553552664271468	23.61496566441953	26.772894148376785	18.05858752293222
12	29.746774303090433	21.694628260762272	30.629936306538546	17.92866112960875
13	24.564904771968635	25.891377316792187	30.83878876541663	18.70492914582255
14	19.613443669526312	28.881962293504564	31.66749136179312	19.837102675176002
15	19.317312734094248	26.630270402978525	36.369856570542225	17.682560292385006
16	22.953311288030363	25.028801053584935	34.81875479050846	17.199132867876248
17	22.969720822771542	25.178975715997776	29.719734427077338	22.131569034153344
18	23.297995885382985	26.818748041085428	31.722710078955597	18.160545994575994
19	25.360324276029466	28.15686334063536	28.097637153548956	18.385175229786217
20	25.638400504856847	27.0820177231412	29.577532520618433	17.702049251383524
21	24.499899391412963	27.078853931095985	31.13044820809146	17.290798469399594
22	23.396410909936122	23.209156604753364	31.988890449692963	21.40554203561755
23	21.054023647447263	26.498572286109727	32.412121457554775	20.03528260888823
24	20.94206759294061	26.69008716458004	33.71741768762236	18.65042755485699
25	21.928327033168774	27.36325774401379	30.9558912549835	19.752523967833937
26	20.20756163172364	29.31493778086564	28.95911663551386	21.518383951896862
27	19.494569456414123	28.97253111378557	32.45143684670331	19.081462583096997
28	18.247993206705722	28.040393609477537	33.72269067436439	19.988922509452355
29	19.557381274485113	27.297746156730884	33.177632580814844	19.967239987969155
30	21.177833376149984	27.37873923308837	32.59528392502572	18.848143465735923
31	23.890046836777437	26.619555693918727	30.566449546164577	18.92394792313926
32	24.3572756460147	26.807442757510525	29.773687627421726	19.06159396905305
33	23.900381890791806	26.656382233325022	29.335945360045862	20.10729051583731
34	20.55148691898541	27.631294206084352	31.023849508114704	20.793369366815533
35	20.69284514756559	28.354115228681	30.238807241962174	20.714232381791238
36	24.291553139262113	28.250385033491902	28.33947741748514	19.118584409760846
37	21.16568441469636	29.60292722476802	29.649709163143257	19.58167919739236
38	22.176832352346878	30.018944786766745	27.66854258443001	20.135680276456366
39	21.03402848172151	28.643285821613595	28.549721944863123	21.77296375180178
40	23.081761245066065	27.46703012309682	28.269367785763187	21.181840846073925
41	20.09898028873188	26.383072784512436	30.126513716304032	23.391433210451652
42	20.701155374671018	27.69840878133683	28.749547050438863	22.85088879355329
43	20.64256194599365	27.526931252486214	29.254783548112627	22.57572325340751
44	20.58848219396745	27.834494023174987	29.424784640675483	22.152239142182083
45	20.31483527400337	29.51510035759287	27.967668576331544	22.202395792072213
46	21.590223123270196	29.232383900432513	27.8385858608868	21.338807115410493
47	20.64509297962982	28.088525432458734	30.207970815494818	21.05841077241663
48	21.054361118598752	27.6610760352033	30.726917078697852	20.557645767500095
49	21.324802062623675	27.283192713322897	30.390669260132462	21.00133596392096
50	20.612358277935336	28.397775558904957	29.90867608801754	21.08119007514217
51	19.132758198123405	29.70281868560892	28.990585820390258	22.173837295877412
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2973.0
1	3709.5
2	4446.0
3	22975.0
4	41504.0
5	28307.0
6	15110.0
7	15063.5
8	15017.0
9	16040.0
10	17063.0
11	17462.5
12	17862.0
13	17487.0
14	17112.0
15	16008.0
16	14904.0
17	14025.0
18	13146.0
19	13026.5
20	12907.0
21	13242.5
22	13578.0
23	14639.5
24	15701.0
25	18228.5
26	21705.5
27	22655.0
28	27737.5
29	32820.0
30	40806.0
31	48792.0
32	53547.0
33	58302.0
34	68174.0
35	78046.0
36	85068.0
37	92090.0
38	99235.5
39	106381.0
40	121028.0
41	135675.0
42	145571.0
43	155467.0
44	161101.5
45	166736.0
46	174808.0
47	182880.0
48	184127.0
49	185374.0
50	176600.5
51	167827.0
52	159989.0
53	152151.0
54	136512.5
55	120874.0
56	115618.5
57	110363.0
58	104645.5
59	98928.0
60	87991.0
61	77054.0
62	65621.5
63	54189.0
64	46873.0
65	39557.0
66	31464.0
67	23371.0
68	19355.0
69	15339.0
70	12594.0
71	9849.0
72	7810.0
73	5771.0
74	4880.0
75	2963.0
76	1937.0
77	1464.5
78	992.0
79	787.0
80	582.0
81	407.0
82	232.0
83	182.5
84	133.0
85	102.0
86	71.0
87	47.0
88	23.0
89	22.0
90	21.0
91	18.0
92	15.0
93	10.5
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2370573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.208189268259215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.79626180968008	17.135424126275186
2	7.163834773988379	3.0386392754656497
3	2.4348110175607545	1.5491379867861386
4	1.3051128220779868	1.1071631898824743
5	0.8713919547181577	0.9240322751250526
6	0.6155515393797563	0.7832840130920511
7	0.4871351589655157	0.7231878255394937
8	0.3768501011176108	0.639384661621193
9	0.3217828395933172	0.6141988228835671
>10	3.3108191766640838	16.398993657550303
>50	1.1968323661417166	18.44169895579775
>100	1.112601300483511	36.21602642902236
>500	0.005211246581661705	0.6866336115864632
>1k	0.0016034604866651402	0.6209585710428438
>5k	0.0	0.0
>10k+	2.0043256083314252E-4	1.1212365983294832
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26377	1.112684570354931	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.21838939361918E-5	0.0	0.0	0.0241713712254379	0.0
2	4.21838939361918E-5	0.0	0.0	0.09335295728079245	0.0
3	4.21838939361918E-5	0.0	0.0	0.1387428271561348	0.0
4	4.21838939361918E-5	0.0	0.0	0.21682521483202585	0.0
5	4.21838939361918E-5	0.0	0.0	0.40758078321148516	0.0
6	4.21838939361918E-5	0.0	0.0	0.6446120832389469	0.0
7	4.21838939361918E-5	0.0	0.0	0.7779553719712492	0.0
8	4.21838939361918E-5	0.0	0.0	1.0153663270441367	0.0
9	4.21838939361918E-5	0.0	0.0	1.12166973976334	0.0
10	4.21838939361918E-5	0.0	0.0	1.3199340412634413	0.0
11	4.21838939361918E-5	0.0	0.0	1.573248324350273	0.0
12	4.21838939361918E-5	0.0	0.0	1.7711329708049488	0.0
13	4.21838939361918E-5	0.0	0.0	1.8566397238136096	0.0
14	4.21838939361918E-5	0.0	0.0	1.9006375251890577	0.0
15	4.21838939361918E-5	0.0	0.0	1.9548860127910004	0.0
16	4.21838939361918E-5	0.0	0.0	2.0679388485399945	0.0
17	4.21838939361918E-5	0.0	0.0	2.1992994942572954	0.0
18	4.21838939361918E-5	0.0	0.0	2.366094610880998	0.0
19	4.21838939361918E-5	0.0	0.0	2.4672094046460495	0.0
20	4.21838939361918E-5	0.0	0.0	2.5803887920768522	0.0
21	4.21838939361918E-5	0.0	0.0	2.733178855913739	0.0
22	4.21838939361918E-5	0.0	0.0	2.900733282628293	0.0
23	4.21838939361918E-5	0.0	0.0	3.081575635932747	0.0
24	4.21838939361918E-5	0.0	0.0	3.2128097299682397	0.0
25	4.21838939361918E-5	0.0	0.0	3.335564861322558	0.0
26	4.21838939361918E-5	0.0	0.0	3.4553249362074063	0.0
27	4.21838939361918E-5	0.0	0.0	3.585462249000558	0.0
28	4.21838939361918E-5	0.0	0.0	3.721547490838713	0.0
29	4.21838939361918E-5	0.0	0.0	3.878176289023793	0.0
30	4.21838939361918E-5	0.0	0.0	4.037378304738981	0.0
31	8.43677878723836E-5	0.0	0.0	4.184304807318737	0.0
32	8.43677878723836E-5	0.0	0.0	4.322921082793063	0.0
33	8.43677878723836E-5	0.0	0.0	4.498448265461557	0.0
34	8.43677878723836E-5	0.0	0.0	4.655245799222382	0.0
35	8.43677878723836E-5	0.0	0.0	4.836172520314709	0.0
36	8.43677878723836E-5	0.0	0.0	4.991029594954469	0.0
37	8.43677878723836E-5	0.0	0.0	5.160355745214343	0.0
38	8.43677878723836E-5	0.0	0.0	5.358451311138699	0.0
39	8.43677878723836E-5	0.0	0.0	5.696428669355468	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGTA	25	3.892064E-5	45.000004	44
GCGTAAG	70	0.0	45.0	1
CGGTCGA	30	2.1664837E-6	44.999996	19
CGATCAT	30	2.1664837E-6	44.999996	10
CGACGGT	595	0.0	44.2437	28
TAGACGG	260	0.0	43.26923	2
CGTTTTT	13630	0.0	43.15114	1
CGGTCTA	605	0.0	43.140495	31
ATGTCGC	65	0.0	41.53846	33
ATCGATC	65	0.0	41.53846	41
GGCGATA	560	0.0	40.98214	8
TTGCGTC	90	0.0	40.000004	16
TAGGGAC	2990	0.0	39.657192	5
ACGTAGG	290	0.0	39.568966	2
CGTTAGG	325	0.0	39.46154	2
TACGGGT	240	0.0	39.374996	4
ACGGGTA	235	0.0	39.255318	5
ATAGGGA	3400	0.0	39.176468	4
TATAGCG	150	0.0	39.0	1
GACGGTC	680	0.0	38.713238	29
>>END_MODULE