FastQCFastQC Report
Thu 26 May 2016
SRR1547339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547339_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3768503
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160003.0781453537386065No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT63120.16749356442067315No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT54970.14586693973707862No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC43070.11428941412544981No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGC700.045.045
CGTTTTT474300.044.2504731
TTAGGCG900.042.5000041
CGACGGT4900.042.244928
ACGGGAC16150.041.6563455
CGGTCTA4900.040.86734831
GGCGATC8300.040.120488
CTACGCG451.9303116E-840.0000041
AATGCGG6600.039.5454522
TAGGGCG10300.039.5388345
GTTTTTT538400.039.2872432
TACGGGA17650.039.135984
CGGGACC13050.038.7931066
TCACGAC5350.038.27102725
GACGGTC5300.038.20754629
TCGTAAG1650.038.1818161
GGGCGAT42800.038.1133167
CGTAAGG5200.038.076922
GCTCGAT659.094947E-1238.0769245
TAAGGGA55800.037.9435464