##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547339_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3768503
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.323527405975263	31.0	31.0	33.0	30.0	34.0
2	31.72301468248798	31.0	31.0	34.0	30.0	34.0
3	31.647251707110225	31.0	31.0	34.0	30.0	34.0
4	35.52308754961851	37.0	35.0	37.0	33.0	37.0
5	35.45867417380323	37.0	35.0	37.0	33.0	37.0
6	35.515953417046504	37.0	35.0	37.0	33.0	37.0
7	35.9505596784718	37.0	35.0	37.0	35.0	37.0
8	35.94886245280951	37.0	35.0	37.0	35.0	37.0
9	37.66454425006428	39.0	37.0	39.0	35.0	39.0
10	37.0184234960142	39.0	37.0	39.0	33.0	39.0
11	36.711473494912966	39.0	37.0	39.0	32.0	39.0
12	36.59047637748995	39.0	35.0	39.0	32.0	39.0
13	36.54420283067308	39.0	35.0	39.0	32.0	39.0
14	37.72434916464177	40.0	36.0	41.0	32.0	41.0
15	37.816591893385784	40.0	36.0	41.0	32.0	41.0
16	37.832814780829416	40.0	36.0	41.0	33.0	41.0
17	37.744629366090464	40.0	36.0	41.0	32.0	41.0
18	37.49700212524708	39.0	36.0	41.0	32.0	41.0
19	37.30740163932469	39.0	36.0	41.0	32.0	41.0
20	37.031645987810016	38.0	35.0	41.0	32.0	41.0
21	36.915528526844746	38.0	35.0	41.0	32.0	41.0
22	36.84991228612529	38.0	35.0	40.0	31.0	41.0
23	36.75178950368356	38.0	35.0	40.0	31.0	41.0
24	36.60542952997517	38.0	35.0	40.0	31.0	41.0
25	36.53103632927982	38.0	35.0	40.0	31.0	41.0
26	36.38361784507004	38.0	35.0	40.0	31.0	41.0
27	36.29567470159902	38.0	35.0	40.0	30.0	41.0
28	36.27538866228845	38.0	35.0	40.0	30.0	41.0
29	36.216775467606105	38.0	35.0	40.0	30.0	41.0
30	36.10867630992996	38.0	35.0	40.0	30.0	41.0
31	35.91374161039543	38.0	35.0	40.0	30.0	41.0
32	35.62919121996188	38.0	35.0	40.0	28.0	41.0
33	35.20630154732529	38.0	34.0	40.0	25.0	41.0
34	34.78019919315442	38.0	34.0	40.0	23.0	41.0
35	34.47226657375621	38.0	34.0	40.0	21.0	41.0
36	34.278262482476464	38.0	33.0	40.0	20.0	41.0
37	34.16743306294303	38.0	33.0	40.0	18.0	41.0
38	34.069355391252174	38.0	33.0	40.0	18.0	41.0
39	34.01318109604795	38.0	33.0	40.0	18.0	41.0
40	33.883008186539854	38.0	33.0	40.0	18.0	41.0
41	33.778460041029554	37.0	33.0	40.0	18.0	41.0
42	33.698780391046526	37.0	33.0	40.0	18.0	41.0
43	33.64628978668718	37.0	33.0	40.0	17.0	41.0
44	33.51220126400324	37.0	33.0	40.0	16.0	41.0
45	33.44217000756003	37.0	33.0	40.0	17.0	41.0
46	33.36046806915107	37.0	33.0	40.0	17.0	41.0
47	33.23874758756992	37.0	32.0	40.0	15.0	41.0
48	33.16526456261279	37.0	32.0	40.0	15.0	41.0
49	33.0420320748053	36.0	32.0	40.0	15.0	41.0
50	32.97910018912019	36.0	32.0	40.0	15.0	41.0
51	32.5955072876418	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	7.0
13	18.0
14	37.0
15	118.0
16	351.0
17	882.0
18	2043.0
19	4012.0
20	7259.0
21	11514.0
22	17354.0
23	26589.0
24	40826.0
25	63705.0
26	91766.0
27	104207.0
28	101762.0
29	98426.0
30	104374.0
31	118038.0
32	140344.0
33	172164.0
34	242232.0
35	331045.0
36	329447.0
37	370945.0
38	526302.0
39	862474.0
40	254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.07608220027953	22.63638903829982	26.501557780370614	22.785970981050035
2	36.86471789991941	23.548581492438775	27.735894067219796	11.850806540422019
3	25.35614274421435	23.647612858474574	38.75119642998825	12.245047967322833
4	22.134863631526898	26.05029105721821	38.77911202405836	13.035733287196535
5	20.319341659008895	29.931222026359006	37.423507424566196	12.325928890065896
6	18.973024567049567	37.30038161041666	34.055591835803234	9.67100198673054
7	80.53898325143963	2.7705165685154025	15.247884902838077	1.442615277206891
8	81.23689963892824	2.2682746968756557	15.01078810339278	1.4840375608033216
9	76.65351467147565	5.0946755250028986	16.09121712255503	2.160592680966421
10	33.59999978771411	35.40220082085645	21.53040610555438	9.467393285875055
11	21.485268818944817	25.31411013869433	36.46811479253167	16.732506249829175
12	22.311618167744594	22.856343752413093	37.1447495199022	17.687288559940114
13	20.74792032804538	24.20255470142919	37.675782664893724	17.373742305631705
14	16.008823662870906	28.66085021028244	37.36205066043466	17.968275466411995
15	15.44597417064548	29.047900452779263	39.91298401513811	15.593141361437155
16	19.276434170279284	27.466290991409586	38.53755722099731	14.719717617313824
17	18.86648358778008	27.620092116153284	35.52622885002347	17.987195446043163
18	18.731045192215586	28.523129741438446	36.07320997223566	16.672615094110313
19	20.015056376497512	30.40947559282824	33.71397077300987	15.861497257664384
20	21.866985378544214	28.93461939661452	33.95995704395087	15.238438180890396
21	21.38997899165796	27.461620701907364	35.93522414603358	15.213176160401092
22	20.48789665286189	25.34749209434091	35.95379916109925	18.21081209169795
23	17.88099943133918	28.235190472184847	36.5042830004381	17.37952709603787
24	17.792768109777278	28.277063863290014	37.37961731753961	16.550550709393093
25	18.404576034568635	29.884811024430658	34.68464798886985	17.02596495213086
26	17.88115864575403	32.079767483268554	33.31508559234263	16.72398827863478
27	17.06667077086047	31.190634583546835	35.31120978277051	16.431484862822188
28	16.694162111586483	30.397295690092324	36.60472076047173	16.303821437849457
29	16.843717518600887	29.22319552352751	36.267929201595436	17.665157756276166
30	17.276966477139595	29.524959911137127	36.07997658486673	17.118097026856553
31	18.829572379271024	29.577792561131037	33.57861198465279	18.014023074945143
32	18.622328282609832	31.01106725933348	33.77924868309778	16.58735577495892
33	18.36042057018397	31.28695930453021	32.61180898622079	17.740811139065034
34	17.287952271764144	31.076822812665934	33.20124728572592	18.433977629843998
35	17.59889802396336	30.711531873531744	32.40920864332601	19.28036145917888
36	18.411342647199696	31.25047266779408	31.42388370129996	18.914300983706262
37	17.912948457252124	31.85835330368584	31.650498885101058	18.578199353960976
38	18.29936184208955	32.095609317545986	30.7094355504029	18.895593289961557
39	17.9915738424515	31.51920006432262	30.98729124004943	19.501934853176447
40	19.292063718670253	30.478070469892156	31.35075121341286	18.87911459802473
41	17.31374500696961	31.256761637180595	31.584637188825376	19.844856167024414
42	18.33911237433007	32.02892501346025	30.15932321136536	19.47263940084431
43	18.933751678053593	31.82152170238421	30.285049527624096	18.959677091938097
44	18.468367943451288	32.470055085533964	30.37487299333449	18.68670397768026
45	18.05817853932981	32.24131704286822	30.220833046968522	19.479671370833458
46	19.103341565603106	32.2363548602721	29.906358041906827	18.753945532217966
47	18.540571680585103	31.163939633323896	31.278574012014847	19.01691467407615
48	18.561932947910616	30.998250498938173	31.324613513641893	19.115203039509325
49	18.01906486474868	30.890860376122824	31.076079812063305	20.013994947065186
50	17.37607745038282	31.262148391549644	32.25392151737706	19.10785264069048
51	16.576529194749217	31.69011673866254	31.48603039456251	20.247323672025736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4191.0
1	4979.5
2	5768.0
3	82624.5
4	159481.0
5	104085.0
6	48689.0
7	48601.0
8	48513.0
9	52288.5
10	56064.0
11	59251.5
12	62439.0
13	61999.5
14	61560.0
15	58668.0
16	55776.0
17	51968.5
18	48161.0
19	44864.5
20	41568.0
21	40565.0
22	39562.0
23	40150.5
24	40739.0
25	43001.0
26	52783.5
27	60304.0
28	68719.0
29	77134.0
30	86145.5
31	95157.0
32	107412.0
33	119667.0
34	130437.5
35	141208.0
36	150987.0
37	160766.0
38	171789.0
39	182812.0
40	197846.5
41	212881.0
42	222645.0
43	232409.0
44	243262.5
45	254116.0
46	268142.0
47	282168.0
48	275482.5
49	268797.0
50	251915.0
51	235033.0
52	206826.0
53	178619.0
54	160870.5
55	143122.0
56	128340.5
57	113559.0
58	105359.0
59	97159.0
60	87586.5
61	78014.0
62	65416.5
63	52819.0
64	41562.0
65	30305.0
66	22790.5
67	15276.0
68	11945.0
69	8614.0
70	6764.0
71	4914.0
72	3887.0
73	2860.0
74	2131.5
75	1039.5
76	676.0
77	589.0
78	502.0
79	352.0
80	202.0
81	175.0
82	148.0
83	90.0
84	32.0
85	26.0
86	20.0
87	17.5
88	15.0
89	12.0
90	9.0
91	7.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3768503.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.94534017917553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.09820553758594	18.940334391598412
2	7.096603205372752	3.3986115573855606
3	2.826032927565399	2.0301095942431435
4	1.644071525549509	1.5747140783271627
5	1.162410663508027	1.3917159382800424
6	0.8844452644875705	1.2707005636809452
7	0.7172930316807633	1.2023077956223605
8	0.5575332614021968	1.068025888438464
9	0.4731450589641432	1.0196657451893225
>10	3.8176321035153977	19.794091597476186
>50	0.972354368169324	16.718744424676295
>100	0.7439649116381922	26.5822493632283
>500	0.0037172971161766656	0.5766355571824139
>1k	0.0022529073431372415	0.984660913787213
>5k	2.2529073431372412E-4	0.31852789291640043
>10k+	1.1264536715686206E-4	3.128904697967859
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116000	3.0781453537386065	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT	6312	0.16749356442067315	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT	5497	0.14586693973707862	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC	4307	0.11428941412544981	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.3071471616182875E-5	0.0	0.0	0.06395112329750036	0.0
2	5.3071471616182875E-5	0.0	0.0	0.17492357044693874	0.0
3	5.3071471616182875E-5	0.0	0.0	0.30654082005507227	0.0
4	5.3071471616182875E-5	0.0	0.0	0.45070947270043304	0.0
5	5.3071471616182875E-5	0.0	0.0	0.830276637699373	0.0
6	5.3071471616182875E-5	0.0	0.0	1.5377458900788987	0.0
7	5.3071471616182875E-5	0.0	0.0	2.023960177290558	0.0
8	5.3071471616182875E-5	0.0	0.0	2.569216476675221	0.0
9	5.3071471616182875E-5	0.0	0.0	2.887008448712924	0.0
10	5.3071471616182875E-5	0.0	0.0	3.2009527390584536	0.0
11	5.3071471616182875E-5	0.0	0.0	3.525882824028533	0.0
12	5.3071471616182875E-5	0.0	0.0	3.78675564275788	0.0
13	5.3071471616182875E-5	0.0	0.0	3.9475622017549146	0.0
14	5.3071471616182875E-5	0.0	0.0	4.045266781000307	0.0
15	5.3071471616182875E-5	0.0	0.0	4.12864206290933	0.0
16	5.3071471616182875E-5	0.0	0.0	4.25099834072044	0.0
17	5.3071471616182875E-5	0.0	0.0	4.398775853435701	0.0
18	5.3071471616182875E-5	0.0	0.0	4.575875354218903	0.0
19	5.3071471616182875E-5	0.0	0.0	4.70425524405845	0.0
20	7.96072074242743E-5	0.0	0.0	4.824329448590063	0.0
21	7.96072074242743E-5	0.0	0.0	4.973433748095729	0.0
22	7.96072074242743E-5	0.0	0.0	5.138539096293674	0.0
23	7.96072074242743E-5	0.0	0.0	5.32463421151582	0.0
24	7.96072074242743E-5	0.0	0.0	5.471827938043303	0.0
25	7.96072074242743E-5	0.0	0.0	5.605037331799922	0.0
26	7.96072074242743E-5	0.0	0.0	5.72837543183593	0.0
27	7.96072074242743E-5	0.0	0.0	5.862885076647146	0.0
28	7.96072074242743E-5	0.0	0.0	6.008035551517406	0.0
29	7.96072074242743E-5	0.0	0.0	6.1696647183244915	0.0
30	7.96072074242743E-5	0.0	0.0	6.343181894773601	0.0
31	1.3267867904045717E-4	0.0	0.0	6.502184023735685	0.0
32	1.3267867904045717E-4	0.0	0.0	6.667793550913983	0.0
33	1.3267867904045717E-4	0.0	0.0	6.825123928520158	0.0
34	1.3267867904045717E-4	0.0	0.0	6.993015528977952	0.0
35	1.3267867904045717E-4	0.0	0.0	7.168124849575547	0.0
36	1.592144148485486E-4	0.0	0.0	7.334132412790968	0.0
37	1.592144148485486E-4	0.0	0.0	7.5127975219868475	0.0
38	1.592144148485486E-4	0.0	0.0	7.6877476281695944	0.0
39	1.592144148485486E-4	0.0	0.0	7.882466857529369	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTCGC	70	0.0	45.0	45
CGTTTTT	47430	0.0	44.250473	1
TTAGGCG	90	0.0	42.500004	1
CGACGGT	490	0.0	42.2449	28
ACGGGAC	1615	0.0	41.656345	5
CGGTCTA	490	0.0	40.867348	31
GGCGATC	830	0.0	40.12048	8
CTACGCG	45	1.9303116E-8	40.000004	1
AATGCGG	660	0.0	39.545452	2
TAGGGCG	1030	0.0	39.538834	5
GTTTTTT	53840	0.0	39.287243	2
TACGGGA	1765	0.0	39.13598	4
CGGGACC	1305	0.0	38.793106	6
TCACGAC	535	0.0	38.271027	25
GACGGTC	530	0.0	38.207546	29
TCGTAAG	165	0.0	38.181816	1
GGGCGAT	4280	0.0	38.113316	7
CGTAAGG	520	0.0	38.07692	2
GCTCGAT	65	9.094947E-12	38.07692	45
TAAGGGA	5580	0.0	37.943546	4
>>END_MODULE